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Showing 1 to 20 of 359 for “"bacteriophage"”.
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Restriction Analysis of Bacteriophage
<p>A restriction map of DNA from bacteriophage P3-C<sub>1</sub> for the endonucleases BamH I, Bgl II, EcoR I, Hind III, Sac I and Sca I is described in this study. The phage DNA was determined to be a linear double stranded molecule with a unit genomic length of 31 kb. After electrophoresis in 0.5% …
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Immunoassays with chemically modified bacteriophage
The immunospecific inactivation of bacteriophage is one of the most sensitive methods available for the detection of very low concentrations of antibody. By chemically modifying the phage coat-protein, this sensitivity can be extended to antibodies against a wide variety of haptens and proteins. …
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CBK Bacteriophage Genome Annotation and Organization
<p>Caulobacter bacteriophages have been isolated from local ponds, streams and the aquaria of fish stores. About three-fourths of these phages belonged to the B3 morphotype with phage CbK being the best studied. As no B3 phage genome sequences are currently available in the Genbank, we decided to …
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Bacteriophage Sp10 Infection of Bacillus Subtilis
Initial phenotypic characterization, methods of phage propagation, and complementation studies were performed with phage SP10 and SP10c, a clear plaque mutant derived from SP10. A propagation scheme was developed to generate high titer lysates of Bacillus subtilis W23. Phage production was analyzed …
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Specific Bacteriophage Coat Protein-Rna Interactions
The coat proteins of the group I bacteriophages translationally repress their replicase genes by binding specifically to a region around the initiation codon called the translational operator. A large number of operator variants have been synthesized by using RNA ligase and transcription of …
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Physical Chemical Studies of Bacteriophage-Lambda
Made available in DSpace on 2014-12-09T17:35:22Z (GMT). No. of bitstreams: 1 6604169.pdf: 5326544 bytes, checksum: b87fcff377168fecbd3f9db64bd586f2 (MD5) Previous issue date: 1965
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Purification and Properties of Bacteriophage f2 Replicase
… during infection of E. coli with the RNA bacteriophage f2. Studies on the RNA replicase of the distantly-related bacteriophage Q3 have established the feasibility of using a simplified assay for replicase activity based on the ability of the enzyme to polymerize GTP in the presence of …
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Transfection and Transformation With Bacteriophage-T4 Dna
Made available in DSpace on 2014-12-11T18:16:50Z (GMT). No. of bitstreams: 1 7212082.pdf: 2102650 bytes, checksum: 1c6e5387798bc6a2aa7e570cca6bac07 (MD5) Previous issue date: 1971
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The characterisation of a novel Xanthomonas Bacteriophage
… pv. campestris was found to be associated with bacteriophage-like particles. The optimal growth conditions for production of the bacteriophage-like particles by the chocolate spot pathogen were determined and a purification protocol developed that yielded particles in a quantity and quality …
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The Genomic Sequence and Annotation of Bacteriophage HK239
Bacteriophages are viruses that infect bacteria and they are the most numerous biological entities on Earth. Temperate phage can adopt two different lifestyles. In the lytic lifestyle, a phage injects its genome into the host and a controlled developmental program ensues. The phage DNA is …
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Targeting Breast Cancer With Bacteriophage Associated Silicon Particles
… particles (S1MP) with targeting entities (bacteriophage) and gold nanoparticles (AuNP) while maintaining their payload carrying potential. Retention of payload carrying potential after the formation of BASP was demonstrated by loading different types of nanoparticles and macromolecules into …
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Characterisation of bacteriophage dynamics associated with foodborne pathogens
Bacteriophages (phages) are viruses that infect bacteria and are found in high abundance in the gut. As antimicrobial resistance rises and interest in microbiome-targeted therapies grows, phages have emerged as potential tools for precisely modulating microbial communities and treating infections. …
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Interfacial behavior of RNA-free MS2 bacteriophage particles
… was investigated in this study. The RNA of bacteriophage MS2 was removed and the empty capsid characterized and compared to intact MS2. Transmission electron microscopy was used to ensure that the MS2 capsid was intact and RNA removed. Electrophoretic mobility, aggregation kinetics and …
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Engineering M13 bacteriophage platforms for cancer therapy applications
Two novel schemes for engineering M13 bacteriophage for application in the diagnosis, imaging and treatment of human tumors are proposed. Firstly, by exploiting the uniquely malleable biology of the M13 filamentous phage, we have engineered filamentous phages of shorter lengths by constructing our …
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Organization and dynamics of the bacteriophage Mu transpososome
… of a linear mobile genetic element, such as the bacteriophage Mu, requires a set of spatially and temporally coordinated DNA phosphoryl transfer reactions to move the element from an existing DNA location to a new DNA site. The Mu transpososome, where the DNA cleavage and joining reactions that …
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Bacteriophage Felix O1: Genetic Characterization and Bioremedial Application
Bacteriophage Felix O1 was studied for applicability as a Salmonella intervention. Felix O1's potential as a Salmonella therapeutic was explored, as was its utility as a food application. Felix O1 is specific for and infects most serovars within the genus Salmonella. The entire 86.155-kb sequence …
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