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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"bacterial two-hybrid"”.
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Shall we dance? : investigating protein interactions implicated in Escherichia coli postreplicative mismatch repair using a bacterial two hybrid system
… patch (VSP) repair. This study made use of the bacterial two hybrid system in an attempt to further clarify the critical role that each of the above-mentioned proteins plays relative to one another. Our initial goal was to determine specific interactions involved in the MMR pathway. Of the six …
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A two-color bacterial two-hybrid system for selecting sequence-specific DNA-binding proteins via fluorescence activated cell sorting
… system (starting with a pre-existing bacterial two-hybrid selection system) which allows the selection of proteins based on their preference of one DNA site over another. I use this system to attempt to attenuate the affinity of a zinc finger protein without destroying its specificity. …
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A PLATFORM FOR THE DISCOVERY AND CHARACTERIZATION OF PROTEINS THAT ASSOCIATE WITH PSEUDOMONAS AERUGINOSA RNA POLYMERASE
… aeruginosa</em> is an opportunistic bacterial pathogen notable for its ability to colonize the lungs of cystic fibrosis patients. Once the bacterium infects and colonizes humans, it is extremely difficult to eradicate. This leads to long-term infections that significantly damage the …
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Characterization of the interaction between FtsZ-ring stabilizer ZapC and the conserved protease ClpXP in E. coli cytokinesis
… that is spatiotemporally regulated during the bacterial cell cycle. Central to the process of division is the assembly of a cytokinetic ring-like structure, termed the Z-ring, formed by polymers of the essential tubulin-homolog FtsZ. Polymerization of FtsZ is regulated by a number of proteins …
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Circadian modulation of the estrogen receptor alpha transcription
… was confirmed to be an interactor of Per2 via bacterial two-hybrid assays, in accordance with previous studies [2]. BRCA1 is a transcriptional activator of ERα promoter in the presence of octamer transcription factor-1 (OCT-1) [3]. Our results indicate that the DNA binding domain of OCT-1, POU, …
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Identification and Analysis of Germination-Active Proteins in Bacillus Spores
… of individual protein domains using bacterial two-hybrid analysis suggested that both N- and C-terminal domains of YpeB contribute to the formation of a multimer. In addition, the uncharacterized YpeB N-terminal domain was demonstrated to have strong self-association and may mediate …
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Investigating proteins that influence membrane-associated germination processes in Bacillus subtilis spores
… that the four lipoproteins appear to interact. Bacterial two-hybrid analysis on individual protein domains further suggests the lipoproteins seem to interact through their predicted ring-building motif within their otherwise uncharacterized domains. Additionally, the absence of the spore lytic …
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Elucidating the roles of the diguanylate cyclases CelR and avmA in attachment and cellulose synthesis with the response regulator divK in Agrobacterium tumefaciens
… study, we attempt to characterize the effects of two diguanylate cyclases (DGC), CelR and AvmA, and other components involved in regulating cellulose production and attachment. CelR positively influences cellulose synthesis through the production of c-di-GMP. This effect occurs when the DGC is …
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The essentiality of DivIVAEf oligomerization for proper cell division in enterococcus faecalis and interaction with a novel cell division protein
… coiled-coil for its proper function in bacterial cell division has not been reported. This study examined the biological significance of DivIVA oligomerization and the N-terminal coiled-coil in bacterial cell division. This research provides evidence that the N-terminal coiled-coil and …
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Evaluating protein-protein interactions of CpsA, a virulence factor in Group B Streptococcus
Streptococcus agalactiae, Group B Streptococcus (GBS), is an important human pathogen, causing life-threatening sepsis and meningitis in newborns. The virulence of GBS in part can be attributed to its ability to produce a polysaccharide capsule. CpsA has been recognized as an integral part of …