Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"bacterial transcription"”.
-
A Structural Perspective on Bacterial Transcription
<p>Transcription is a highly conserved process that lies at the very center of biology. As in the other two domains of the tree of life, bacterial transcription is a multistep process. In the first step, RNA polymerase (RNAP) contacts with the promoter DNA are established and then must be broken …
-
Conditional Protein Alleles and Regulation of Bacterial Transcription: A Thesis in Two Acts
… for the study of biological processes. The bacterial RNA polymerase σ factors are required for promoter specific transcription initiation. The σ factors responsible for driving most gene expression during log phase growth, known as the group 1 σ family are autoregulated by a poorly …
-
Deconstructing Ihf-Mediated Inhibition of the Complex acs Promoter
… starvation. As befits a survival gene, its transcription is subject to complex regulation. Previously, the Wolfe lab reported that CRP activates acs transcription by binding tandem DNA sites located upstream of the major acsP2 promoter and that the nucleoid protein IHF binds three specific …
-
Prion biology in the context of bacteria
… that prion-like mechanisms are operative in the bacterial domain of life. In what follows, we demonstrate that Escherichia coli cells can propagate a model yeast prion in a chaperone-dependent manner, establishing that the bacterial cytoplasmic milieu in general and a molecular machine in …
-
Structural and Functional Studies of the Unconventional Proteobacterial Transcription Activator Crl in Complex with the Transcription Machinery
… with the core RNA polymerase to initiate the transcription of genes needed to survive different environmental changes. Crl is a small protein that activates the transcription of σ<sup>S</sup>-dependent genes. In contrast to most transcription activators, Crl does not bind DNA to help recruit …
-
Structure-Function Analysis of the EsaR N-terminal Domain
… family is a class of quorum-sensing regulated bacterial transcription factors that alter gene expression as a function of ligand detection. This coincides with a high population density and/or a low rate of signal ligand diffusion. The majority of LuxR proteins are activated only in the …
-
Energy Landscapes for Protein Folding
… transition of the C-terminal domain of RfaH, a bacterial transcription factor. PEL techniques are employed to construct the free energy landscape (FEL) for the refolding process and to discover mechanistic details of the transition at an atomistic level. The final part of the thesis focuses on …
-
Mechanistic and Engineering Studies of LOV Photoreceptors in Yeast
… (HTH) DNA-binding activity of the bacterial transcription factor EL222 and the Regulator of G-protein Signaling (RGS) in the fungal membrane associating protein BcLOV4 are both induced by blue-light sensing LOV domains. While previous work has provided the field with a structural …
-
Cryo-EM Studies of Bacterial RNA Polymerase
… a single RNA polymerase (RNAP) performs all transcription. The overall structure of bacterial RNAP resembles a crab claw with pincers comprising the β' and β subunits and a large cleft where the active site sits. Structural information about this essential enzyme has mainly been provided by …
-
Dynamics of Cell Fate Decision Making Between Sporulation and Competence in Bacillus Subtilis
… The master regulator of sporulation is the transcription factor Spo0A. Single cell measurements of Spo0A dynamics along with activities of stage-specific sporulation reporters Spo0F, SpoIIE and SpoIIR revealed the reversible and noisy progression of sporulation up until the final …