Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"bacterial toxins"”.
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Effects of heat stress and fescue toxicosis on the immune system and other physiological parameters
… fungi also grow on these grasses which produce toxins, resulting in reduced feed intake (FI), body weight (BW) and a compromised thermoregulatory system when consumed by animals . Our first study showed that sensitizing rats helped them adapt to the toxin by maintaining a lower core body …
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Biochemical and Structures Studies of tRNA Modificaton and Repair Enzymes
… repaired full length tRNAs cleaved by bacterial toxins (colicin E5 and D). Before the broken RNAs were ligated, a methyl group was added to the 2' OH group that participated in the original RNA cut. Due to this methylation, RNAs repaired by bacterial Pnkp/Hen1 could not be cleaved again …
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REGULATED UPTAKE OF BIOPOLYMERS Role of cell surface proteoglycans Implications for drug and gene delivery
… of microbes and pathological factors such as bacterial toxins and viral proteins. The HIV-1 TAT protein was shown to enter cells and to target their nuclei, thus acting as a paracrine transcription factor, a finding that initiated the field of so called cell penetrating peptides (CPPs). Due to …
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SURFACE PLASMON RESONANCE IMAGING BIOSENSORS FOR THE DETECTION OF PATHOGENS AND TOXINS IN FOOD
The detection of pathogens and toxins in food represents an essential requirement for food quality control. Standard methods for pathogen detection rely on laborious and time-consuming growth of pathogens in different culture media followed by biochemical or serological identification. Such methods …
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DEVELOPMENT OF SURFACE BASED PLATFORMS FOR BIOMOLECULAR RECOGNITION AND PROTEASE ASSAYS
… were engineered in order to develop assays for bacterial toxin activity. Bacterial toxins are comprised in part of proteases, which selectively cleave peptides bonds in proteins. In this thesis we present, protease assays from our main focus of studies, the Clostridium botulinum Neurotoxin type …
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Comparative analysis of cytotoxic necrotizing factor (CNF) toxins: Compatibility of cargo with delivery vehicle and identification of amino acid residues that modulate pH-dependent cytosolic cargo delivery
AB toxins are modular proteins that deliver their catalytic cargo into the cytosol of host cells. These toxins are increasingly being exploited for targeted delivery in biomedical and research applications due to their potency and cell specificity. This thesis explores the suitability of the …
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Structural Analysis of Cycle Inhibiting Factor from Pathogenic Escherichia Coli
<p>Bacterial pathogens have evolved a sophisticated arsenal of virulence factors to modulate host cell biology. Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) use a type III protein secretion system (T3SS) to inject microbial proteins into host cells. These translocated …
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Application of NMR spectroscopy to interrogate the structures of bacterial protein toxin membrane localization domains and amphotericin B
… localization domains (MLD) of two different bacterial toxins. The MLDs studied were from the C-terminal domain of the AB toxin Pasteurella multocida toxin (PMT), which is expressed by Pasteurella multocida, and the Ras/Rap1-specific endopeptidase domain of the multifunctional autoprocessing …
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Effect of Autoregulated TxeR on the Expression of <I>Clostridium difficile</I> Toxins
… tissue damage caused by two of the largest known bacterial toxins, A and B. Molecular studies of the C. difficile toxins have identified a 19.6 kb toxigenic element that contains both toxin genes flanked by three small open reading frames (ORFs). The focus of this study is to elucidate the …
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Computational Approaches To Anti-Toxin Therapies And Biomarker Identification
… work describes the fundamental study of two bacterial toxins with computational methods, the rational design of a potent inhibitor using molecular dynamics, as well as the development of two bioinformatic methods for mining genomic data. </p> <p>Clostridium difficile is an opportunistic …
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Analysis of Vibrio parahaemolyticus Virulence Systems
… cysteine protease domain of several large bacterial toxins. Mutations of conserved catalytic residues and of residues in the putative IP6-binding pocket abolished toxicity in yeast. Furthermore, VPA1380 was not toxic in yeast cells deficient for the production of IP6. Therefore, our …
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Protein Sensors for Membrane Cholesterol
… recently discovered class of cholesterol-sensing bacterial toxins share the same specificity and sensitivity for cholesterol as mammalian cholesterol sensors. Using two members of this large family, perfringolysin O and anthrolysin O, I showed that sigmoidal responses of cholesterol sensors can …
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Aspects of sepsis/SIRS - An experimental study on fluid therapy, vitamin C and plasma volume in increased permeability
… of the capillary endothelium due to e.g. bacterial toxins, cytokines, pro-inflammatory hormones and free oxygen radicals. This will result in leakage of plasma fluid to the interstitum with subsequent intravascular hypovolemia and potentially harmful tissue oedema. Restoration of plasma …
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Detection of staphylococcal enterotoxins in patients with rheumatoid arthritis or closed fracture
… develop protocols to digest staphylococcal enterotoxins B and C (SEB/SEC), toxic shock syndrome toxin 1 (TSST-1) and alpha haemolysin (AH), members of the pyrogenic toxin superantigen family (PTSAg). To develop a novel mass spectrometry method to analyse and compare urine samples from patients …
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Cellular interactions of the cytolethal distending toxins
… system, pathogenic effector proteins, called toxins, have exploited existing pathways as a mean to access intracellular targets and modulate host cellular functions. Among known targets in the human host, nucleus has been an infrequent, covert, and a coveted target, and very few external …
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Characterization of vacuolating cytotoxin A of helicobacter pylori in cell binding and cellular activity
The sensitivity or resistance of host cells to bacterial toxins is often dependent on the presence or absence of cell surface toxin receptors required for the binding and subsequent downstream action of the toxin. Our laboratory has identified plasma membrane sphingomyelin (SM) to be important for …
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Identification and Characterization of the Helicobacter Pylori Vacuolating Cytotoxin Receptor
… in the intoxication pathway of essentially all bacterial toxins, and provides the basis for development of novel drugs to block the action of toxins. Here, we identify VacA as the first bacterial virulence factor that exploits the important and abundant plasma membrane sphingolipid, …
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Reversibly neutralized perfringolysin O for intracellular delivery of macromolecules/
… of an intracellular delivery system based on a bacterial pore-forming protein, and demonstrate its efficacy in vitro using protein and siRNA payloads. Perfringolysin O (PFO) is a member of the cholesterol-dependent cytolysin (CDC) family of bacterial toxins whose pores, reaching up to 30nm in …
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Characterization of the Type III Effector VOPA from Vibrio Parahaemolyticus
Vibrio parahaemolyticus is a marine bacterium and causative agent of gastroenteritis associated with the consumption of contaminated seafood. It is endemic to Southeast Asia and is the leading cause of gastroenteritis in Japan. Sequencing of the Vibrio parahaemolyticus genome revealed the presence …
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Untersuchungen zur Substratspezifität der ADP-Ribosyltransferasen Clostridium botulinum C2-Toxin und Clostridium perfringens Iota-Toxin
Clostridium perfringens Iota-Toxin und Clostridium botulinum C2-Toxin gehören zur Familie der binären Aktin-ADP-ribosylierenden Toxine. Die Toxine bestehen aus einer Enzymkomponente (ADP-Ribosyltransferase) und einer separaten Bindungs- und Translokationskomponente, die die Zellaufnahme vermittelt. …
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