Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"bacterial toxin"”.
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Selective molecular transport in nanopore systems
… the mechanism of selective probe capture in bacterial toxin protein α-hemolysin (aHL) using dielectrophoresis. Third, I introduce the first simulation of molecular artificial water channel pillar[5]arene (PAP) and uncover the mechanics of its water transport and self-aggregation properties. …
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Variability of Cholesterol Accessibility in Human Red Blood Cells Measured Using a Bacterial Cholesterol Binding Toxin
… anthrolysin O, a cholesterol-binding bacterial toxin, to measure accessible cholesterol in human red blood cells (RBCs). Accessible cholesterol levels were stable within individuals, but varied >10 fold among individuals. Significant variation was observed among ethnic groups …
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Construction of inducible expression systems for controlled transfer of Agrobacterium tumefaciens T-DNA in plant transient expression systems /
… virulence factor virE2 and expression of toxin pemK and antitoxin pemI from bacterial toxin-antitoxin (TA) module pemIK in Agrobacterium that provides for strictly regulated T-DNA transfer from agrobacteria to model plants Nicotiana benthamiana. The study provided detailed information …
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High-Throughput Screening Development Using Photonic-Crystal Optical Biosensor
… that are DNA sequence-dependent (the bacterial toxin-antitoxin system MazEF), and those that are DNA sequence-independent (the human apoptosis inducing factor (AIF)). The PC technology was further utilized in a screen for inhibitors of the AIF-DNA interaction, and through this screen …
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Investigation of the YefM-YoeBSa toxin-antitoxin systems as novel antibacterial targets
… continued discovery and development of new antibacterial compounds. Bacterial toxin-antitoxin (TA) systems consist of a toxin capable of arresting cell growth and an antitoxin that binds to and inhibits the toxin under normal circumstances. Cellular stress causes a shift in the toxin/antitoxin …
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The genetic landscape of protein-protein interaction specificity
… and degeneracy of protein interfaces. Using bacterial toxin-antitoxin systems as a model, we use a variant of deep mutational scanning to dissect the positive and negative contributions of antitoxin residues that dictate toxin specificity. By screening a combinatorially complete library of …
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DEVELOPMENT OF SURFACE BASED PLATFORMS FOR BIOMOLECULAR RECOGNITION AND PROTEASE ASSAYS
… were engineered in order to develop assays for bacterial toxin activity. Bacterial toxins are comprised in part of proteases, which selectively cleave peptides bonds in proteins. In this thesis we present, protease assays from our main focus of studies, the Clostridium botulinum Neurotoxin type …
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Comparative analysis of cytotoxic necrotizing factor (CNF) toxins: Compatibility of cargo with delivery vehicle and identification of amino acid residues that modulate pH-dependent cytosolic cargo delivery
AB toxins are modular proteins that deliver their catalytic cargo into the cytosol of host cells. These toxins are increasingly being exploited for targeted delivery in biomedical and research applications due to their potency and cell specificity. This thesis explores the suitability of the …
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Acinetobacter baumannii toksino-antitoksino sistemos CheTA antitoksino veikimo mechanizmo paieška /
… of Activity of Acinetobacter baumannii Toxin-antitoxin System Antitoxin Acinetobacter baumannii is a gram negative opportunistic pathogen which causes variety of nosocomial infections. Due to the ability to persist in clinical environment and rapidly acquire antibiotic resistant …
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Application of NMR spectroscopy to interrogate the structures of bacterial protein toxin membrane localization domains and amphotericin B
… localization domains (MLD) of two different bacterial toxins. The MLDs studied were from the C-terminal domain of the AB toxin Pasteurella multocida toxin (PMT), which is expressed by Pasteurella multocida, and the Ras/Rap1-specific endopeptidase domain of the multifunctional autoprocessing …
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Cellular interactions of the cytolethal distending toxins
… system, pathogenic effector proteins, called toxins, have exploited existing pathways as a mean to access intracellular targets and modulate host cellular functions. Among known targets in the human host, nucleus has been an infrequent, covert, and a coveted target, and very few external …
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The Origin of Multiple Sclerosis Revisited: The Case for a Soluble Toxin
… of this thesis, I will propose that a soluble bacterial toxin, Clostridium perfringens epsilon toxin (ETX), may be the long sought environmental trigger for MS. ETX is a neurotoxin with a unique tropism for the brain tissues that are specifically damaged during acute MS exacerbations, the …
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Structural and functional characterization of a Xanthomonas Type III effector
Rice bacterial leaf streak disease caused by Xanthomonas oryzae pv. oryzicola (Xoc) is one of the most important rice bacterial diseases. Xanthomonas type III effector gene avrRxo1 is conserved in diverse Xoc strains and its homologues have been identified from several other gram-negative bacteria …
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Effect of Temperature and Chemical Additives on the Efficacy of the Herbicides Glufosinate and Glyphosate in Weed Management of Liberty-Link and Roundup-Ready Soybeans
… is a synthetic analog of a naturally occurring bacterial toxin, it was tested for possible bactericidal activity on the soybean pathogen Pseudomonas syringae. Greenhouse and field-studies showed that the 5 weeds responded differently to glufosinate and glyphosate. Common milkweed was the most …
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The role of microbial-mediated genotoxicity during pathogenesis
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01
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Understanding the molecular mechanism on cell surface receptor interaction of helicobacter pylori vacuolation cytotoxin
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-12-01
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Immunodulation of inflammation in a murine pnemococcal sepsis model
… at different time points (6h, 12h and 24h) after bacterial challenge for bacteriological examinations, serum cytokine measurements, and biochemical assays for liver function. Spleens were harvested for flow cytometric analysis of splenic lymphocytes or NK activation. Innate stimulation with LPS …