Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 195 for “"bacterial cells"”.
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Establishing how bacterial cells position the division site
… both temporally and spatially to ensure that cells divide precisely at the centre between segregated chromosomes. Failure to do so can lead to cell death. The earliest event in bacterial cell division is the polymerization of the highly conserved tubulin-like protein, FtsZ, to form a …
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Phenotypic Switching of Bacterial Cells in Extreme Environments
… conditions lead to stresses on mesophilic cells, and therefore, application of these environments may reveal the bottleneck cellular processes that are prone to fail. The effect of pressure, temperature, and salinity on various cellular processes including metabolisms, growth, cell …
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A model for sigma factor competition in bacterial cells
… response genes. In exponentially growing E. coli cells, the majority of the transcriptional activity is carried out by the housekeeping sigma factor, while stress responses are often under the control of alternative sigma factors. Different sigma factors compete for binding to a limited pool of …
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Comparison of induced proteins synthesized in hydrogen peroxide and near-ultraviolet -treated bacterial cells
I examined whether bacterial protein synthesis regulation in response to near-ultraviolet radiation (NUV) and hydrogen peroxide (H2 O2 ) occurs via common or separate biochemical pathways. My approach was to compare the proteins that were induced by the two agents, as well as the proteins that …
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Evaluating strategies for integrating bacterial cells into a biosensor designed to detect electrophilic toxins
… plants, the construction of a whole-cell bacterial biosensor is explored to harness the natural stress response of the bacterial cells. The stress response selected in this work is the glutathione-gated potassium efflux (GGKE) system, which responds to electrophilic stress by effluxing …
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Removal of colour from solutions of azo dyes using bacterial cells (Shewanella strain J18 143)
… the graft copolymer was controlled at 5.5%. The bacterial cells were immobilised by three methods. The first method, "growing-in", was carried out by growing the bacterial starter culture with the presence of graft cellulosic copolymer. The cells were therefore bound to the substrate. The …
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Use Of Cryoprotectants, Prebiotics And Microencapsulation of Bacterial Cells in Improving the Viability of Probiotic Organisms in Freeze-Dried Yoghurt
The main aim of this study was to improve the viability of probiotic organisms within freeze-dried yoghurt during processing and storage. Initially, probiotic organisms including Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus and Bifidobacterium spp. were incorporated in …
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Use of cryoprotectants, microencapsulation of bacterial cells and acid and low temperature adaptations in improving viability of probiotic bacteria in fermented frozen dairy desserts
The main aim of this study was to improve the viability of probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium spp.) in fermented frozen dairy desserts. In order to improve the viability of probiotic bacteria, initial studies were undertaken to determine the existing levels of …
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Role of disinfectants and pipe materials on bacterial adhesion onto biofilms
… The fact that biofilms can protect attached bacterial cells from disinfectants raises rudimentary questions regarding interactions of bacterial cells with biofilm surfaces. Consequently, the main objectives of this study were to: 1) investigate the mechanisms that govern E. coli S17, E. coli …
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Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining
… host-pathogen interaction and distribution of bacterial cells in vivo. Most of these processes however have only been monitored in murine models or mammalian tissue culture systems. In order to determine whether BLI could be used to monitor bacterial-host interactions in an invertebrate system, …
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Gene Inhibition Using Genetic Suppressor Elements in Escherichia Coli JM109DE3 Cells
… study was to determine if gene inhibition in bacterial cells could be achieved by isolating GSEs from a genomic library. Additionally, if it was possible to use GSEs to inhibit gene function in E. coli JM109DE3 cells, could this method be used to locate and determine the function of unknown …
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Bacterial transport and horizontal gene transfer in subsurface: multiple-scale experimental studies
Horizontal gene transfer rapidly changes bacterial genetic repertoire and contributes to bacterial evolution. Natural transformation, one mechanism of horizontal gene transfer, is defined as the process by which bacterial cells successfully take up and incorporate extracellular DNA. The …
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Measurement of Efficiency of Cre-mediated Recombination between lox loxSites Using a New Vector System
… were transformed into Cre-expressing BS1365 bacterial cells and grown at 37℃ overnight respectively. Plasmid DNA was isolated from the culture of each BS1365 colony. The purified DNAs were plated out on LB agar containing ampicillin, X-gal, and IPTG. Recombination efficiency was measured by …
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Bacterial extracellular polymers and flocculation of activated sludges
… produced by bacteria play an important role in bacterial aggregation or bacterial flocculation in secondary waste treatment. The mechanisms responsible for this floc formation are thought to be polymer induced adsorption and interparticle bridging among bacterial cells or between bacterial cells …
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Evaluation of Surface Acetylated Bacterial Cellulose for Antibacterial Wound Dressing Applications
… arise due to infection by pathogenic bacteria. Bacterial hydrolytic enzymes degrade the host tissue while biofilms can shield the bacterial cells from the host's immune response. Wound dressings with bacteriostatic or bactericidal properties are a promising solution. This study investigated the …
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Catalase, Superoxide Dismutase, and Staphylococcus Aureus Virulence
… These oxygen-free radicals are known to kill bacterial cells. The aim of this study was to investigate the relationship between catalase and superoxide dismutase (the enzymes that degrade hydrogen peroxide and superoxide radicals, respectively) and virulence of Staphylococcus aureus.
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Sensitive Detection of Foodborne E. coli O157:H7 by Dendrimer-aptamer Modified Microchannels - with RCA Signal Intensifications
Foodborne pathogenic bacterial infection is one of the main causes for food poisoning cases. Therefore there is an urgent need for rapid, sensitive and cost-effective detection methods in order to avoid or to respond to outbreaks of such diseases in a timely manner. To this end, a dendrimer-aptamer …
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Understanding and exploiting bacteriophage-host interactions for the control and detection of listeria monocytogenes
… of infection, the growth stage of the host bacterial cells and storage temperature for the successful application of phages as biocontrol agents. Chapter 3 of this thesis also aimed to exploit phages (phage lysins) as biocontrol agents. The key findings showed that polyhydroxyalkanoate …
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Comparative pathogenesis of Yersinia enterocolitica biotypes
… that Y. enterocolitica pathogenic biotypes bacterial cells were growing inside the Galleria larvae while the non-pathogenic biotype bacterial cells were dying. The heat-killed Y. enterocolitica strains were completely avirulent to the Galleria larvae.
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Aeromonas bakteriofago KLEA5 uodegėlės baltymo gp45 tyrimai /
… problem due to the growing prevalence of bacterial resistance. In addition, bacteria can form biofilms which act as a virulence factor and prevent drugs from reaching the cell surface, further complicating the issue. The use of bacteriophage-encoded depolymerases offers a promising avenue …
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