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Showing 1 to 20 of 21 for “"bacterial artificial chromosome (BAC)"”.

  1. Klonierung und Mutagenese des Bovinen Herpesvirus Typ 1 als ein infektiöses künstliches bakterielles Chromosom (bacterial artificial chromosome, BAC)

    Trapp, SaschaLudwig-Maximilians-Universität

    … Schönböken genome was cloned as an infectious bacterial artificial chromosome (BAC), by inserting mini F plasmid sequences into the glycoprotein E (gE) open reading frame (ORF). DNA of the resulting BAC clone pBHV-1DgE was transfected into permissive bovine kidney cells and infectious BHV-1DgE …

    lmu-germany Repository record

  2. Haemobartonella Felis (Mycoplasma Haemofelis) Molecular Diagnostics and Genomic Studies

    Berent, Linda MarieUniversity of Illinois at Urbana-Champaign2015

    … kb using pulsed field gel electrophoresis. A bacterial artificial chromosome (BAC) was used to create a large insert library of the H. felis genomic DNA. Using hybridization experiments and fingerprinting analysis, these BACs were ordered into a genomic encyclopedia with a restriction map. The …

    uiuc Repository record

  3. Sequence characterization of the HSA12/22/12 evolutionary breakpoint region on SSC5q21

    Pierce, NatalieUniversity of Illinois at Urbana-Champaign2012

    … map constructed by Meyers and colleagues. Bacterial artificial chromosome (BAC) fingerprint analysis and radiation hybrid mapping of BAC-end sequences was used to construct a minimum tiling path (MTP) encompassing each breakpoint region. The MTP clones were sequenced. The MTP sequences were …

    uiuc Repository record

  4. Construction of a Simian Immunodeficiency Virus Vaccine from Cynomolgus Macaque Cytomegalovirus

    Russell, Justen Norman Hoffman2018

    … macaque cytomegalovirus was first cloned as a bacterial artificial chromosome (BAC) to facilitate the preservation of the virus without further accumulation of attenuations from in vitro passaging. After sequencing the BAC, and comparison of the growth kinetics of the viruses derived from the …

    toronto-retro Repository record

  5. Characterization and Development of a Novel Cytomegalovirus for Evaluation as an HIV Vaccine Viral Vector in a Non-Human Primate Model

    Marsh, Angie2014

    … virus for vaccine purposes, I cloned CyCMV as a Bacterial Artificial Chromosome (BAC). The cloning of CyCMV-BAC enabled the virus to be transformed into a bacterial system for downstream insertion of vaccine antigens, as well as future viral manipulations, including attenuations. This newly …

    toronto-retro Repository record

  6. Characterization of malus germplasm and QTL mapping of fruit quality traits

    Potts, Sarah M.University of Illinois at Urbana-Champaign2010

    … A total of 502 SSRs derived from three sources, bacterial artificial chromosome (BAC) end sequencing, expressed sequence tag (EST) sequences, and previously published markers, were used to genotype progeny. Linkage groups were mapped, and despite genotype by environment interactions and sometimes …

    uiuc Repository record

  7. Molecular genetics and functions of Kaposi's sarcoma-associated herpesvirus glycoproteins in viral entry and virus-induced cell fusion

    Luna, Rafael E.Biological Sciences2022

    … full-length KSHV genome has been cloned into a bacterial artificial chromosome (BAC) and successfully recovered in 293 cells. The herpesviral glycoproteins are structural components of the KSHV particle and are thought to facilitate virus entry, egress and virus-induced cell fusion. …

    lsu-thes Repository record

  8. QTL Mapping and Molecular Dissection of Meat Quality Traits in Swine

    Meyers, Stacey N.University of Illinois at Urbana-Champaign2015

    … map was used, together with porcine linkage and bacterial artificial chromosome (BAC) physical maps, to fine-map a QTL affecting pork tenderness that was detected, on the q arm of porcine chromosome 2 (SSC2q), within the Illinois Meat Quality Pedigree (IMQP). Nine novel microsatellite markers …

    uiuc Repository record

  9. Two Odorant-Binding Protein Genes in Mosquitoes: Comparative Genomics, Expression, and Function

    Sengul, Meryem SenayVirginia Tech2008

    … in Anopheles quadriannulatus by screening bacterial artificial chromosome (BAC) libraries of these species. Comparative analyses revealed several interesting features including segments of conserved non-coding sequences (CNSs) that contain potential regulatory elements relevant to olfactory …

    vt Repository record

  10. Tetracycline-dependent gene expression in cholinergic neurons of transgenic mice : a tool to study Neuregulin-1 function in vivo

    Fischer, Tobias MarcUniversität Heidelberg

    … all required regulatory elements were present, a bacterial artificial chromosome (BAC), roughly 200kb in size, containing the ChAT gene and flanking sequences was used. Homologous recombination in bacteria was used to insert the tTA2S cDNA into the ChAT gene. The resulting ChAT-tTA2S BAC construct …

    heid-diss Repository record

  11. Genetic Regulation of Müllerian Duct Regression

    Moses, Malcolm, <p>https://orcid.org/0000-0003-1851-3632</p>2023

    … specific transcriptional enhancers, we utilized bacterial artificial chromosome (BAC) transgenic mice. <em>Osx</em> and <em>Amhr2</em> are two genes expressed in the MD mesenchyme which are essential for proper regression. Both genes are located on the same chromosome within regions covered by …

    uthsc Repository record

  12. Functional genomic analysis of cell cycle progression in human tissue culture cells

    Kittler, RalfTechnische Universität Dresden

    … including its regulatory sequences carried on a bacterial artificial chromosome (BAC). This technology allows physiological expression of the transgene, which cannot be achieved with conventional cDNA expression constructs. The use of the orthologous gene from a closely related species confers …

    qucosa-diss

  13. Chemical genetic manipulation of interferon regulatory factor 1 (IRF-1) using synthetic biology

    Al Samman, Khaldoon Mohammed A, Alsamman, KhaldoonThe University of Edinburgh2012

    … using recombineering technology, of universal bacterial artificial chromosome (BAC) targeting vector. This targeting vector, carry a cre-conditioned STOP cassette that can be targeted at a desired specific area. The resulted targeting vector can aid the generation of mice models with a …

    edinburgh Repository record

  14. Molecular analysis of the sex-determining region of the Y chromosome in the platyfish Xiphophorus maculatus

    Zhou, QingchunUniversität Würzburg

    … Xiphophorus maculatus has three types of sex chromosomes (X, Y and W; females are XX, WX or WY; males are XY or YY). Its gonosomes are at an early stage of differentiation. The sex-determining locus on the sex chromosomes is flanked by two receptor tyrosine kinase genes, the Xmrk oncogene and …

    wurz-thes Repository record

  15. A molecular investigation of the novel gene underlying autosomal dominant retinitis pigmentosa in a South African family

    Bardien-Kruger, SorayaDivision of Human Genetics1999

    … African adRP families to a 10 cM interval on chromosome 17q22. This novel locus, designated RP17, is the eighth identified for adRP. Haplotype construction in the two kindreds, in conjunction with multipoint analyses subsequently fine mapped RP17 to a 1 cM region between microsatellite markers …

    cape-town Repository record

  16. Synaptic Protein Profiling in the Mammalian Brain

    Heller, Elizabeth

    … In order to achieve this, we have used the BAC (bacterial artificial chromosome) transgenic approach to target particular neurons for expression of a given neurotransmitter receptor fused to an affinity tag. Immunohistochemistry of fixed brain tissue confirmed the correct localization of …

    rockefeller Repository record

  17. Analysis of the antifreeze glycoprotein containing genomic locus in the Antarctic notothenioid fish dissostichus mawsoni

    Nicodemus Johnson, Jessie D.University of Illinois at Urbana-Champaign2010

    … The AFGP/TLP locus was isolated by screening a bacterial artificial chromosome (BAC) library for AFGP/TLP positive clones. Seven BAC clones representing two haplotypes encompassed the AFGP/TLP locus. Assembly of the AFGP/TLP locus was complicated by its highly repetitive nature. Thus, an …

    uiuc Repository record

  18. Creating sense from non-sense DNA: de novo genesis and evolutionary history of antifreeze glycoprotein gene in northern cod fishes (gadidae)

    Zhuang, XuanUniversity of Illinois at Urbana-Champaign2014

    … origins of the gadid AFGP. A large-insert DNA bacterial artificial chromosome (BAC) library for each species was constructed and screened to isolate the AFGP-containing clones. Nine BAC clones of B. saida were selected by fingerprint analyses and sequenced on Roche 454 GS FLX+. Sequence …

    uiuc Repository record

  19. BAC transgene arrays as a model system for studying large-scale chromatin structure

    Bian, QianUniversity of Illinois at Urbana-Champaign2011

    … regions consisting of tandem repeats of BAC transgenes, which contain 150-200 kb of defined genomic regions, and used them as a model system to study the mechanisms and functional significance of large-scale chromatin organization. The BAC transgene arrays recapitulated several important …

    uiuc Repository record

  20. Changes in large-scale chromatin structure and dynamics of BAC transgenes during transcriptional activation

    Hu, YanUniversity of Illinois at Urbana-Champaign2010

    Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2010-05-14T20:52:51Z Item is restricted until 2012-05-14T20:52:43Z

    uiuc Repository record

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