Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 65 for “"avidity"”.
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Modulating Avidity of H8F4-Car By Regulation of Expression Via Codon Modification
… and strategy for modulating the affinity/avidity of CAR therapies by introducing rarer, or less frequently used, codons that encode the same residue. Data from on-going studies in which rare synonymous codons were introduced show that expression of h8F4-CAR can be altered. While MFI values …
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Differentiation into Effector Cells with Distinct Memory Fates: Contributions of Viral Dose and Functional Avidity
… presented here examines two factors; functional avidity and viral dose and how they contribute to effector cell differentiation. The data presented here show that at the peak of the effector response generated following poxvirus or LCMV infection, SLEC were of higher functional avidity than their …
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Characterisation of high and low avidity peptide specific CD8+ T cells using immunologic, transcriptomic and proteomic tools
… immunotherapy is the generation of high avidity cytotoxic T cells which can recognise and respond to very low concentration of antigens. This sensitivity of T cells is usually determined by peptide titration ELISpot assays. Even though these assays are generally useful, they are laborious …
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Visualizing the dynamics of HIV-specific cytotoxic T-cells in extracellular matrix
… targets. Using this approach, we found that high-avidity CTLs engaged, arrested, and killed the first target encountered with near-perfect efficiency. These CTLs remained in contact with dead targets for hours, accumulating TCR signals and upregulating antiviral cytokine and chemokine secretion …
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Hapten Binding by Lymphocyte Surface Immunoglobulin and the Subsequent Implications for Affinity Maturation
… from multi-determinant-smIg interaction (avidity) may provide a degree of enhancement in antigen binding as to obscure the selective advantage of intrinsic smIg affinity. Second, in the young New Zealand Black animal model, characterized by B lymphocyte hyperactivity (possibly due to T(,s) …
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Quantitative analysis of the T cell receptor signaling network in response to altered peptide ligands
… in different cell fates, based in part on the avidity of the ligand. To address the question of ligand affinity discrimination in T cells, several T cell lines, both mouse and human, were screened for their ability to exhibit multiple cell fates in response to stimulation through the T cell …
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DEVELOPMENT OF A SUBUNIT LAWSONIA INTRACELLULARIS VACCINE
… Antigens group. Further, when measuring antibody avidity of the Montanide 61 + Antigens sera, GroEL and YopN antibodies had better antibody avidity than FliC antibodies. Thus, antibody isotyping and avidity can be utilized to further drive informed vaccine development choices. The outcome of this …
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DEVELOPMENT OF BISPECIFIC T CELL ENGAGERS TARGETING CARBONIC ANHYDRASE 9 FOR THE IMMUNE THERAPY OF CLEAR CELL RENAL CELL CARCINOMA
… by 3-fold through a novel application of avidity sensors, while reducing potency by 33-fold against some RCC cells and dPBTE efficacy by nearly 2-fold in mouse models. However, providing 2:1 CA9 bivalency with a novel Persistent Multivalent T Cell Engager (PMTE) format restores avidity, …
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Human herpesvirus 6 in multiple sclerosis and encephalitis
… We developed an immunofluorescence IgG-avidity assay for the detection of primary HHV-6A and HHV-6B infection. For HHV-6B antibodies, no differences were observed between patients with MS and OND. For HHV-6A both seroprevalence and mean titers were significantly higher in MS compared to …
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Synthesis and properties of novel cage-functionalized crown ethers and cryptands.
… experiments the cryptand exhibited high avidity towards Rb+ and Cs+, when compared with the corresponding model compound. A computational study of a series of cage-functionalized cryptands and their alkali metal-complexes was performed. The X-ray crystal structure of a K+-complexed …
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The Use of High-Affinity T Cell Receptors to Study Specificity in the 2C T Cell System
… TCRs with enhanced properties, such as increased avidity and signal amplification, to detect antigen. The results show that tetrameric and multi-fluorophore labeled forms of the high-affinity 2C TCR called m67 were capable of detecting low levels of specific peptide-MHC on the surface of both …
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Investigating cross-clade immune responses in HIV-1 subtype C-infected individuals from South Africa: implications for HIV vaccine design
… relevant peptide concentrations by functional avidity studies using peptide dilution IFN-γ ELISpot assays. Additionally, the cytokine profile, cytotoxic potential and proliferative capacity of cross-reactive peptide variants was characterised using multiparameter flow cytometry. Results The …
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Using biomarker data to monitor the HIV epidemic
… biomarkers of antibody response, such as LAg Avidity, provide reliable estimates of the probability of recency. The proposed methods are applied to a panel of individuals for whom information on various biomarkers is given along with an estimated date of detectable infection. At population …
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Identification of the factors impacting immunosorbent performance
… to yield a highly pure product. The customized avidity of the parent antibody for a given antigen or hapten can make immunoaffinity chromatography an indispensable tool in the field of bioseparations. However, the use of immunoaffinity chromatography at a large scale is frequently precluded by …
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The problem of evil in early modern English literature
… Hutchinson, and John Milton) shed light on the avidity of interest and diversity of approach early modern writers brought to questions of evil. Bringing philological evidence from the literary archive to bear on existing historical-theoretical theses regarding the relationship between early …
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Design, Synthesis, And Testing Of Multivalent Compounds Targeted To Melanocortin Receptors
… multivalent construct should show greater avidity towards melanoma cells than healthy cells due to the synergistic effects arising from multivalency. Both oligomeric and shorter linear constructs bearing the minimum active sequence of melanocyte stimulating hormone, His-DPhe-Arg-Trp-NH₂ …
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Recognition of hepatitis B epitopes by T cell receptors and T cell receptor-like antibodies
… of the TCR, I developed a sensitive bead-based avidity assay. Using this assay, a comparison between the TCR tetramer and the Env183-191 specific monoclonal antibody showed that although the tetrameric form of the soluble TCR had similar functional avidity for the Env183-191 pMHC as the …
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Über den Beitrag von Valpha- und Jalpha-Segmenten des T-Zellrezeptors von CD8 T-Zellen der Ratte bei RT1f-spezifischer Alloreaktion und positiver Selektion im Thymus
… (TCR)/pMHC-Komplexes und dem "differential-avidity model" der TCR-vermittelten Antigenerkennung bei thymischer Selektion und Aktivierung reifer T-Zellen. Sie dokumentieren die Bedeutung der Erkennung polymorpher MHC-Strukturen durch keimbahnkodierte TCR-Segmente und sprechen gegen eine …
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CYTOTOXIC EVALUATION OF SOME MANNICH BASES OF CONJUGATED STYRYL KETONES
… with antineoplastic activity and increased avidity for thiols compared to their precursors. Several analogs of Mannich bases were designed on the basis of some of the established approaches in rational drug designing.
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Integrin Modulating Factor 1 (IMF-1): A lipid that modulates leukocyte integrins
… factor (IMF-1), that may serve to control CR3 avidity. Addition of IMF-1 to cells or to purified CR3 causes enhanced binding of ligand. IMF-1 cannot be extracted from resting PMN but can be extracted from cells within one minute of stimulation with a variety of agonists (PMA, TNF, formylated …
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