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Showing 1 to 20 of 53 for “"avidin"”.
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Metabolic biotinylation of the adenoviral capsid: Avidin-based applications and studies of ligand-targeted gene delivery
… to the Ad capsid, based on the extremely strong avidin-biotin interaction, is described herein. The genetic insertion of a biotin acceptor peptide (BAP) into the fiber, protein IX, or hexon components of the Ad capsid has resulted in vectors that are metabolically biotinylated upon production in …
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Cuticular Proteins of Hyalophora Cecropia: Characterization With Electrophoresis, Antibodies, and Lectins (Insect, Hydrophogicity, Metamorphosis, Glycoproteins, Avidin-Biotin)
… and 2D gels with biotinylated lectins and the avidin-biotin-complex method. More cuticular proteins from flexible cuticular regions than from rigid cuticles were glycosylated, but in both cases these were minor proteins as detected by Coomassie Blue. These proteins contained primarily …
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Chicken Liver Pyruvate Holocarboxylase: Studies on Its Synthesis From Theapoprotein and Biotin and Its Protection by Sulphate Ions Against Avidin Inhibition
Made available in DSpace on 2015-05-12T21:45:25Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7021012.PDF: 2670037 bytes, checksum: 380d5a20657f22fd632c692e2f5d4566 (MD5) Previous issue date: 1970
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Cartilage stress relaxation induced by intra-tissue transport of cationic nanoparticles : implications for post-traumatic osteoarthritis drug delivery
Avidin has previously been proposed as a drug carrier molecule for intra-articular injection of osteoarthritis (OA) therapeutics. Its nanoscale size and net positive charge make it a viable candidate for transport into and through articular cartilage, one of the most affected tissues in OA. Avidin …
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Charge-based transport and drug delivery into cartilage for localized treatment of degenerative joint diseases
… Based on this understanding we selected Avidin, a globular protein, as a drug carrier owing to its optimal size and high positive charge (66,000 Da, pI 10.5). Avidin resulted in a six-fold upward Donnan partitioning factor at the synovial fluid-cartilage interface, had a 400-fold higher …
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Genetic engineering for pest resistance in potato (Solanum tuberosum L.)
… to transform two potato cultivars with an avidin gene anticipated to confer insecticidal activity against certain lepidopteran insects. A successful gene transfer into plants requires a high regeneration response of transformed cells of the target explant used for plant transformation. …
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ΡΑΔΙΟΔΕΣΜΕΥΤΙΚΗ ΑΝΑΛΥΣΗ ΓΙΑ ΤΟΝ ΠΡΟΣΔΙΟΡΙΣΜΟ ΒΙΟΤΙΝΗΣ ΣΕ ΒΙΟΛΟΓΙΚΑ ΥΓΡΑ
… 125I-PHENYL)ETHYL] BIOTINAMIDES (RADIOTRACER),AVIDIN (BINDING PROTEIN), AND A DOUBLE AVIDIN ANTIBODY (SEPARATION REAGENT). THE ASSAY IS SIMPLE AND FAST (IT ALLOWS HANDLING OF >100 SAMPLES IN <4 H), REPRODUCIBLE (INTA- AND INTER ASSAY CV 41% AND 70%, RESPECTIVELY), SENSITIVE (8 NG/L), SPECIFIC …
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New inverse hydogel opals as protein responsive sensors
… a test system, biotin as a recognition unit for avidin was coupled to the IHO via polymer-analogous Steglich esterification. The amount of accessible biotin was quantified with a colorimetric binding assay. When avidin was added to the biotinylated IHO, the wavelength of the opalescent reflection …
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Development of Ultra-Sensitive Fluorescence Photoamplification Assays for the Detection of Molecular Recognition Events
… events. Proof of concept utilized biotin-avidin recognition, where avidin is coupled to the photochemical sensitizer.</p>
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Development of Protein Based PCBP2 siRNA Nanocomplex for Liver Fibrosis Therapy
… and Objectives. In chapter 2, we reviewed the avidin-biotin technology and its potential applications in nanotechnology, therapy and diagnosis. We also discussed the challenges and biological barriers for siRNA delivery. In Chapter 3, we rigorously investigated the intracellular barrier which …
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Microfluidic Devices for Bioanalytical Applications
… in fabrication and optimization of biotin-avidin protein bioassay. In order to optimize the performance of protein bioassay, various substrates and protein blocking reagents have been tested. Silicon dioxide substrate with proper thickness provide a great option for build biotin-avidin …
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Macromolecule Loading on PLGA Particles through Surface Adsorption
… <p>The specific binding between avidin and biotin (or desthiobiotin) was employed to enable surface adsorption. The pair has the strongest known non-covalent affinity, an interaction that is relatively insensitive to temperature and pH. PLGA-biotin particles were prepared by …
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Development of luminescent probes for ultrasensitive detection of biopolymers, their complexes, and living cells
… lanthanide probes to a carrier molecule, avidin. The resulting avidin conjugates were used for imaging of living cells employing time-gated fluorescent microscopy. Six thiol-reactive derivatives of the lanthanide ion probes with bromoacetamido- and maleimido- cross-linking groups were …
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Development of Bioassays Based on Nucleic Acid Amplification and Next-Generation Sequencing
… to self-assembled, ultra-detectable DNA-avidin nanoparticles stabilized with poly(ethylene glycol) to link DNA amplification to target protein concentration. Electrostatic neutralization and steric cloaking of the PCR-amplifiable DNA labels by avidin and PEG coating reduces non-specific …
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Entwicklung neuer stimuli-sensitiver Hydrogelfilme als Plattform für die Biosensorik
… Der Einfluss des biotinbindenen Proteins Avidin auf das thermoresponsive Verhalten des Copolymers in Lösung wurde untersucht. Die spezifische Bindung von Avidin an das biotinylierte Copolymer verschob die Übergangstemperatur deutlich zu höheren Temperaturen. Kontrollversuche zeigten, dass …
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Engineering, overexpression, purification and analytical applications of recombinant bioluminescent reporters.
… of aequorin from the crude cell extract using an avidin resin and (b) the direct complexation of aequorin with streptavidin for utilization as a reporter molecule. Streptavidin-biotinylated aequorin complexes were used for the quantification of biotinylated hybrids in a hybridization assay, with …
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Steered molecular dynamics: a tool to investigate molecular interactions
… of 40 to 600 ps, the unbinding of biotin from avidin by means of external harmonic forces with a variety of loading rates. The applied forces reduced the overall binding energy sufficiently to yield unbinding within the measurement time. The simulations revealed a variety of unbinding pathways, …
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Ανοσοϊστοχημική μελέτη της έκφρασης των οιστρογονικών υποδοχέων στους όγκους των ωοθηκών με μονοκλωνικά αντισώματα (με την μέθοδο αβιντίνης-βιοτίνης)
… 6 non-neoplasmic cysts of the ovaries, using the avidin-biotin-peroxidase complex (ABC) method and monoclonal anti-ER antibodies. The estrogen receptors (ER) were localized in 35% of cystadenomy in 67% of tumors of borderline malignancy and in 66% of carcinomas; also in 67% of sex cord-stromal …
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Epidermal growth factor receptor in equine gastric stratified squamous mucosa: effect of progressive ulceration on receptor density
… locations at post-mortem examination and an avidin-biotin immunoperoxidase technique was developed to stain the formalin-fixed tissue for EGFr. A computerized image analysis system was used to measure EGFr area and mean intensity values at four sites within the epithelium from the basal cell …
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Influence of protein and lipid domains on the structure, fluidity and phase behavior of lipid bilayer membranes
… membranes that bind the proteins streptavidin and avidin. Fluorescence microscopy studies demonstrate that proteins crystallized in a single layer on lipid bilayer surfaces can change the lipid curvature and stabilize lipid vesicles against osmotic collapse.
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