Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"auxotrophs"”.
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Genetic and phenotypic analyses of auxotrophs requiring aromatic amino acids and vitamins in Streptomyces coelicolor
Five mutants of Streptomyces coelicolor having auxotrophic requirement for aromatic amino acids were mapped relative to closely linked genetic markers. Based on four-point reciprocal crosses mutants were found to map in at lea.st two locations. One mutant mapped between proA and hisC while three …
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Molecular-genetic characterisation of arginine auxotrophy in the opportunistic human pathogen, Pseudomonas aeruginosa
… including the emergence of amino acid auxotrophs. Arginine auxotrophs arise at high frequency in CF, and arginine is known to play an essential physiological role in Pa, so I chose to investigate the possible molecular basis of this further. I found that a transposon (Tn) mutant …
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Cloning and characterization of MET2 in Pichia pastoris
… and have also constructed strains with various auxotrophs including me/2.</p>
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Fatty acid metabolism of marine microalgae
… sp. and Crypthecodinium cohnii. PUFA-requiring auxotrophs of both of these algae were created and used as tools for studying PUFA biosynthetic pathways. Additionally, equilibrium radio-labeling techniques were applied to algal cultures fed 14C-fatty acids. Both organisms were found to possess …
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Omics-driven and constraint-based modelling of microbial community metabolism
… a high frequency of predicted amino acid auxotrophs across microbial communities in a large dataset, with a higher percentage of total auxotroph relative abundance in host-associated compared to free-living samples, as well as evidence for higher drug tolerance in auxotrophs. In the second …
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Impaired virulence factor production in a dihydroorotate dehydrogenase mutant (pyrD) of Pseudomonas aeruginosa.
… research consolidates the idea that pyrimidine auxotrophs cause decreased pathogenicity in P. aeruginosa. Such a finding may open the search for chemotherapy targets in cystic fibrosis and burn victims where P. aeruginosa is an infecting agent.
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Characterization of the Broad-Spectrum Racemase, BsrF, in Vibrio fischeri
… on BsrF. I also found, using amino acid auxotrophs, that BsrF is required for growth of an L-Met auxotroph on D-Met, an L-Lys auxotroph on D-Lys, and an L-Leu auxotroph on D-Leu. Additionally, BsrF is sufficient to confer D-Lys utilization in an Escherichia coli L-Lys auxotroph. Through …
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Exploring the Biophysical Properties of Domains Within Multi-Domain Proteins
… 7- zatryptophan (7AW), using E.coli Trp auxotrophs. Biophysical analysis shows that incorporation of 7AW does not significantly perturb the structure or function of the isolated domain. Ligation of 7AW labeled SH3 domain to the c-Crk-I Src homology 2 (SH2) domain, via EPL, generated the …
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Alternative Processing of SREBP in Site 2 Protease and Scap Mutants During Larval Development in Drosophila Melanogaster
… despite these organisms being cholesterol auxotrophs. dSREBP is essential for activating genes involved in the uptake and synthesis of fatty acids which are required for rapid growth during larval development. I have demonstrated that processing of SREBP in Drosophila does not require the …
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Alternative Processing of SREBP in Site 2 Protease and Scap Mutants During Larval Development in Drosophila Melanogaster
… despite these organisms being cholesterol auxotrophs. dSREBP is essential for activating genes involved in the uptake and synthesis of fatty acids which are required for rapid growth during larval development. I have demonstrated that processing of SREBP in Drosophila does not require the …
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EPR and solid-state NMR studies on the mechanism of cytochrome bo3 ubiquinol oxidase from escherichia coli
… bo3 samples were prepared from C43(DE3) auxotrophs for solid-state NMR (SSNMR) experiments. The initial SSNMR spectra proved that clean isotope labeling at the selected amino acid types was achieved through the use of auxotroph strains. This selective labeling approach dramatically …
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Metabolic Coordination of Stem Cell Fate Controls Tumor Initiation and Tissue Repair
… initiation, I find oncogenic EpdSCs are serine auxotrophs, whose growth and self-renewal require abundant exogenous serine. When extracellular serine is limited in vitro and in vivo, EpdSCs activate de novo serine synthesis, which in turn produces the metabolite α-ketoglutarate (αKG). αKG …
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A METE mutant of Chlamydomonas reinhardtii provides new perspectives on the evolution of vitamin B12 auxotrophy
… experiment illustrates that newly-evolved B12 auxotrophs could survive in the presence of B12producers, but it was also demonstrated that M. loti could not produce sufficient B12 to favour the evolution of B12 dependence in C. reinhardtii. In summary, this work has provided an insight into the …
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Comparative whole genome transcriptional responses of Ruminococcus albus strain 7 and 8: a case for specialization and niche differentiation
… synthetic pathway are likely autotrophs and not auxotrophs. Notably, the potential long non-coding RNA (lncRNA) loci was identified in the genomes of R. albus strains. The putative lncRNA loci consisted of four sequence components; lncRNA, DUF1292 gene, putative 6S RNA, and alcohol dehydrogenase. …