Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"auxin-inducible degron (AID)"”.
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The Cell-Essentiality of KAT7 in Acute Myeloid Leukemia
… to sustain the leukemic programme. Using the auxin-inducible degron (AID) system to induce rapid KAT7 protein degradation, I showed that KAT7 is required for the recruitment of the MLL-fusion associated adaptor proteins such as BRD4 and AF4 to gene promoters, which are critical for the …
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Identification of EWS-FLI1 Regions Necessary for Ewing Sarcoma Proliferation
… cells. This report shows the adaptation of two inducible degron systems, Small Molecule Assisted Shut-Off (SMASh) and Auxin-inducible degron (AID), into the endogenous locus of EF in a series of Ewing sarcoma cell lines to define the phenotype of EF depletion. Across multiple cell line and …
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MYC-DEPENDENT TRANSCRIPTIONAL CONTROL IN HUMAN LYMPHOMA
… we inserted an in-frame cassette encoding an Auxin-inducible degron (AID) into the oncogenic MYC allele of three human B-cell lymphoma cell lines, thus directing expression of a conditionally degradable MYC-AID fusion protein. Treatment of the cells with Auxin caused an immediate MYC-AID drop, …
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Analysis of the functions of phosphoprotein phosphatase 1 catalytic subunits during cell division
… I investigated the validity of using the auxin response factor (ARF)-auxin inducible degron (AiD) system for targeted proteolysis in hTERT-RPE-1 and found that this system resulted in leaky degradation of the target protein. I next tested the AiD2 system to study PP1 phosphatases and …
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Signalling and transcriptional regulation of human primordial germ cell specification
… versus PGC specification. To achieve inducible loss of function, I deleted endogenous PRDM14 in the background of doxycycline-repressible PRDM14 transgene expression. This approach, however, did not allow fast and homogenous control over protein levels. To circumvent this, I used …