Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"autophagic flux"”.
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Doxorubicin Inhibits Cardiomyocyte Autophagic Flux by Suppressing Lysosomal Acidification
… I showed that doxorubicin blocks cardiomyocyte autophagic flux in vivo and in cardiomyocytes in culture. This block was accompanied by robust accumulation of undegraded autolysosomes. Moreover, I went on to localize the site of block as a defect in lysosome acidification. To test the functional …
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Autophagy gridlock in tauopathy
… and to study the effects of human tau on the autophagic machinery. We showed that caloric restriction and rapamycin feeding suppressed the human tau-induced toxic phenotype, with a decrease in the soluble levels of human tau. Genetic upregulation of the autophagy/lysosomal system also …
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Scalable methods for the discovery of autophagy and disease genes
… two high-throughput autophagy assays: (1) an autophagic flux assay, SRAI-LC3B; and (2) an assay for levels of the ATG12-ATG5 conjugate, an important early autophagy marker. SRAI-LC3B can be used to assess autophagic flux via flow cytometry or microscopy and responds robustly to established …
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Differential Autophagic Responses to Nano-Sized Materials
… of cellular clearance or blockage of the autophagic flux and, eventually, cell death. Due to its important role in maintaining cellular homeostasis and survival, defects in the lysosome-autophagy system may have deleterious effects on cells, possibly leading to pathologic conditions. The …
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Resistance to HER2-targeted therapies in HER2-positive breast cancer
… overexpression in SKBR3 cells revealed increased autophagic flux and resistance to HER2-targeted therapies. Inhibition of autophagy reversed resistance, rendering them susceptible to sapatinib- and lapatinib-induced cell death. Further, increased autophagic flux was seen in all progressive tumours …
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The unfolded protein response regulates hepatocellular injury during the pathogenesis of nonalcoholic steatohepatitis
… induced hepatotoxicity by inhibiting autophagic flux. Saturated FFA treatment activated the UPR, including the transcription factors CHOP (GADD153/DDIT3) and NF-κB, leading to increased expression and secretion of cytokines such as TNFα and IL-8 that contributed to hepatic cell death …
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The physiological role of P2X4 receptors in lysosome function
… suggesting that P2X4Rs mediate lysosomal Ca2+ efflux downstream of P2X7R stimulation. Next, I showed that the expression of P2X4R was sufficient to enhance the cytosolic Ca2+ response to the activation of endogenous histamine H1 receptors and to promote lysosome fusion. Similar results were …
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Understanding Neuronal Autophagic Lysosomal Pathways Through Genetic and Pharmacological Perturbations
… JIP3 is critical for preventing accumulation of autophagic vacuoles during retrograde axonal transport and that loss of JIP3 is associated with decreased mitochondrial and synaptic proteins in axons. Further, our results suggest that JIP3 has functions in the endolysosomal system in the soma. …
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Directed Evolution of Macrocyclic Peptides For Inhibition of Autophagy
… of the adapter protein p62 suggesting decreased autophagic flux through successful disruption of LC3 PPIs in cell culture. In mouse models of metastatic ovarian cancer, treatment with LC3-binding SUPR peptides and carboplatin substantially reduced tumor growth after four weeks of treatment. These …
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Μελέτη του ρόλου των εξωσωματίων των μεσεγχυματικών στρωματικών κυττάρων στην οξεία ηπατική ανεπάρκεια
… στην αποκατάσταση της αυτοφαγικής ροής (autophagic flux), η οποία εμφανίζεται ανεσταλμένη στα πρότυπα μοντέλα ΟΗΑ. Η παρατηρούμενη μείωση των επιπέδων της p62 σε συνδυασμό με τη ρύθμιση του λόγου LC3-II/I υποδηλώνει ότι η MFGE-8 δεν προκαλεί απλώς την έναρξη της αυτοφαγίας, αλλά …
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Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
… drug-treated OS cell lines showed increase autophagic flux (LC3-I/LC3-II protein conversion) that correlated with reduced p62/SQSTM1 expression, analysed by Western Blot. RT-PCR results confirmed this pattern. Combination of chloroquine (CQ) with chemotherapy had a significant effect on OS …
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Inhibition of the autophagy pathway modulates osteosarcoma chemoresistance
… chemotherapy was confirmed by an increased autophagic flux and autophagic clearance of p62. Autophagy-related microRNA (miRNA) expression was found to be decreased in chemotherapy-treated HOS-143B cells. To investigate the role of the key autophagy gene ATG7, CRISPR/Cas9 technology was …
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INHIBITING THE NUCLEAR RECEPTOR ESRRA RESCUES CORE PATHOLOGIES IN CELLULAR AND ANIMAL MODELS OF BATTEN DISEASE
… enhances cellular clearance by boosting autophagic flux by mechanism independent of the nuclear translocation of the master gene of lysosomal biogenesis and autophagy TFEB. To validate these findings in a living organism, we treated CLN7 knockout mice with XCT-790 via dietary …
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Myc influences glutamine metabolism to induce autophagy in tumorigenesis
… produce macromolecules, by modulating glycolytic flux, glutaminolysis, lipidogenesis, and autophagy. The process by which hyperproliferative cells undergo metabolic reprogramming to sustain growth can be recapitulated in the epithelial cells from Drosophila imaginal discs, where different levels …
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Repression Of Autophagy During Erythroid Development By Gfi1b
… as a vital strategy in preventing excessive autophagic flux and cell death during erythroid development. These results provide key insights in understanding the balanced regulation of autophagy during mammalian erythropoiesis.</p>
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Characterisation of Novel Autophagy and Necroptosis Antagonists Encoded by Human Cytomegalovirus
… lysosomal acidification, a process essential for autophagic cargo degradation. By overlapping quantitative proteomic data from HCMV-infected cells with a database of vacuolar acidification proteins, Dmx-like protein-1 (DMXL1) was identified as the only factor that regulates vacuolar acidification …
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On connections between metazoan cellular metabolism and cell size
… pathway to RAB11 was found to regulate basal autophagic flux, thus providing a novel connection from lipid biosynthesis to other growth regulating processes.<br/><br/>In conclusion, this thesis provides evidence for cell size dependent metabolism, where mitochondrial functions do not increase …
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The role of autophagy in protecting periodontium integrity, and lipopolysaccharide-induced osteoclast differentiation
… to tensile strain undergo an altered state of autophagic flux which contributes to osteogenesis. In vivo and in vitro models of tooth movement were used to analyse osteogenic and autophagy marker expression. Initially tensile strain was found to increase autophagy but decrease expression of …
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RNA binding proteins in neurotoxic protein clearance
… for proteolytic degradation. Dysfunction in the autophagic machinery or in the lysosomes is a hallmark of numerous neurodegenerative diseases, including Parkinson’s disease (PD). Deficient clearance of damaged cellular components, misfolded proteins, and aggregates leads to the accumulation of …
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The regulation of CD8+ T cells by nutrient availability
… used that allowed for quantitative analysis of autophagic flux at the single cell level in T lymphocytes. Using this fluorescent reporter naïve T cells were shown to have high levels of basal autophagy, and this was required to maintain cell survival and expression of the surface molecule …
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