Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"autolysis"”.
-
Postmortem energy metabolisms' role in calpain activation, inhibition, and subsequent function
… calpastatin abundance, and calpain-1 autolysis in the aforementioned system. In LTL, calcium addition significantly enhanced calpain-1 autolysis and desmin degradation in 1-day samples suggesting that calcium availability is rate-limiting to muscle proteolysis in 24 hr aged beef …
-
Genetic studies on Clostridium acetobutylicum
… at pH 4.6 and 37°C. Optimum conditions for autolysis and autoplast formation in C. acetobutylicum were defined. Autolysis-deficient mutants which produced less autolysin than the parent strain were isolated. Growth of the P262 strain and the lyt-1 mutant was inhibited by the same …
-
Optimized GeLC-MS/MS for Bottom-Up Proteomics
… such as weak thermostability and fast autolysis at basic pH. Glycosylated trypsin derivates maintained their cleavage specifity and showed better thermostability, autolysis resistance and less autolytic background than unmodified BT. In line with the “accelerated digestion protocol” …
-
Overcoming long-standing challenges in recombinant protein expression
… across scales and allows for autoinduction and autolysis. More importantly, we are able to decouple growth from protein production and show that this platform is easily adaptable to other applications such as (i) reduction of essential housekeeping proteases to enable production of …
-
Influence of copper on resistance of Lumbricus terrestris to bacterial challenge
… that Cu++ exposure caused coelomocyte death, autolysis and release of agglutinins into the coelom, possibly explaining resistance to bacterial challenge.
-
A Study of the Morphological Development of Some Filamentous Fungi in Tower Fermenter Culture, with Particular Reference to Aspergillus Niger
… of colony development from inoculation to autolysis and included the growth of giant colonies and re-growth. Rheological work involved the study of the ascent of air slugs in a long tube containing mould broth. For comparison, some fermentations involving two dimorphic fungi, Mucor …
-
Structural Studies of the Receiver Domain of LytR from Staphylococcus Aureus and Interaction Studies of TraW and TrbC from the F Plasmid of Escherichia Coli
… such as sporulation, chemotaxis, as well as autolysis that leads to biofilm formation. In addition, microbes utilize T4SSs to deliver DNA, protein substrates, toxin, and virulence factors, from a donor to a recipient cell. These systems are complex, versatile and have a significant impact to …
-
The Role of the Ubiquitin-Proteasome Pathway During Xylem Differentiation in <I>Zinnia elegans</I> Mesophyll Cells and <I>Arabidopsis thaliana</I>
… 48 h with LLL resulted in partial uncoupling of autolysis from differentiation. Results of protease activity gel analysis suggest that incomplete autolysis was due to the ability of LLL to inhibit TE cysteine proteases. A characterization of phytohormone-stimulated growth of non-differentiating …
-
Mode of Action of Penicillin in Pneumococci: From Inhibition of Cellular Targets to Bacterial Death
… suggesting that mechanisms other than bacterial autolysis may exist for the cidal effect of this antibiotic. We have investigated the existence of such autolysis-independent lethality in pneumococci. The results of these experiments are presented in four Chapters to be summarized next. Chapter I …
-
Yeast flocculation: understanding cell surface structure-function relationships in industrial yeast strains
… ageing can cause off flavours due to yeast autolysis. Despite this commercial interest, limited information is available about the onset of flocculation and the various factors that may be responsible in the process. In particular, what are the signals that trigger flocculation? Adhesion …
-
Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana
… synthesis, programmed cell death, and cellular autolysis. The end result of tracheary element differentiation is a cellular corpse lacking a protoplast and consisting of a thickened cell wall composed mostly of lignin and cellulose. Little is known about the genetic mechanisms regulating the …
-
Some aspects of the ultrastructure and enzyme cytochemistry of normal and virus-transformed cells
… modification through a process of sublethal autolysis by lysosomal enzymes. Examination of GERL morphology and enzyme activity gives an insight into the functioning of the system and provides evidence of possible mechanisms of cellular autophagy.
-
Toxic Effects of an Anionic Detergent on the Gills of Trout
… cause death in isolated cells. These are autolysis, i.e. lysis by the action of the cells' own enzymes, induced by an initial lesion in the cell membrane whose precise nature is not known; and rapid lysis by the direct action of the detergent on the cell constituents. The occurrence of …
-
Analysis of the streptococcal cpsa protein in dna-binding and regulation of capsule and cell wall maintenance
… with altered antimicrobial resistance and autolysis activity in S. iniae. Taken together, CpsA contributes to the complex regulatory scheme controlling capsule and cell wall, the two major constituents of bacterial cell surface macromolecular structure, and does so in a way that influences …
-
Hypertrophic scar therapy : pressure-induced remodelling and its determinents
… ischaemia in scars, implying remodelling by autolysis, was investigated with vital microscopy using a hamster cheek pouch model. Pressure magnitudes which induced scar remodelling did not disturb the microcirculation sufficiently to cause permanent damage, therefore this hypothesis was …
-
The development and validation of an ABL835 FLEX Blood Gas Analyser method for the quantitation of glucose, lactate, and electrolytes in post-mortem vitreous humour
… it less prone to the effects of putrefaction and autolysis after death. As such, VH has frequently been described as the specimen of choice in biochemical forensic investigations to identify potential ante-mortem conditions and to estimate the post-mortem interval. The lack of a validated method …
-
Function and Regulation of Xylem Cysteine Protease 1 and Xylem Cysteine Protease 2 in Arabidopsis
… terminated by programmed cell death and cellular autolysis. The final two processes are probably dependent on the activity of hydrolytic enzymes such as XCP1 and XCP2 known to be expressed in tracheary elements during these final two processes. Thus, the transcriptional regulation of XCP1 and the …
-
Retention of Nutrients and Phytochemicals in Fresh and Processed Broccoli
… and 73.6%, 32.2%, 76.2%, 77.9%, respectively.) Autolysis reduced the formation of CHB and SF (80.0% and 18.9%, respectively) and increased SFN and IN (38.4% and 31.1%, respectively). The initial activity of myrosinase decreased significantly (p $<$ 0.01) during two weeks of storage from 19.2 to …
-
Nervenzupfpräparation (nerve fiber teasing) in der Diagnostik peripherer Neuropathien beim Tier
… of phenomena seen with pathology, artefacts or autolysis. This categorization substantially increases objective diagnosis when evaluating teased fibers. Additionally, clinical signs were correlated with their neuropathological findings. In order to improve the separation of nerve fibers, the …
-
The structure of the nitrilase from Rhodococcus Rhodochrous J1: homology modeling and three-dimensional reconstruction
… lost – either by specific proteolysis or autolysis - an active helix is formed.
Page 1 of 2