Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"assembly factors"”.
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Chemical Biology, Biochemical and Structural Studies of MDN1, an AAA Protein Required for Ribosome Biogenesis
… requiring a plethora of more than 200 unique assembly factors. Energy-harnessing enzymes, such as ATPases and GTPases, are needed to remodel the precursors of ribosomes at fast time scales. Mdn1 is an essential dynein-like AAA protein (ATPases Associated with various Activities) that releases …
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Structural Studies of the Nucleolar Stages of Ribosome Biogenesis in Yeast
… cell therefore requires the timely and accurate assembly of functional ribosomal subunits, a complex process termed ribosome biogenesis. In addition to the ribosomal RNAs and ribosomal proteins that make up the mature subunits, eukaryotic cells require over 200 trans-acting factors to assemble …
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Making Ribosomes: Biochemical and Structural Studies of Early Ribosome Biogenesis in Yeast
… process involving more than 200 trans-acting factors. Ribosome assembly begins in the nucleolus where RNA polymerase I transcribes a long polycistronic RNA, the 35S preribosomal RNA which contains the sequences for three of the four ribosomal RNAs, as well as spacer sequences which are …
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Mechanisms and Evolution of Mitoribosomal Small Subunit Biogenesis
… between mRNA codon and tRNA anti-codon. The assembly of the ribosome is a complex process which is tightly controlled to generate functional subunits which exhibit high translational accuracy and processivity. During ribosome biogenesis, ribosomal proteins and a multitude of additional …
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Identification and characterisation of new factors and mechanisms regulating human cytochrome c oxidase biogenesis
Assembly of the mitochondrial complex IV (CIV) or cytochrome c oxidase (COX) is an intricate and highly regulated process in which the three-core mitochondrial DNA (mtDNA) encoded subunits assemble in a coordinated way with the remaining eleven supernumerary nuclear DNA (nDNA) encoded subunits. …
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The Evolution of Ribosome Assembly: Making Ribosomes in the Mitochondria
… a comprehensive understanding of their assembly pathways remains elusive. This thesis presents the first structural characterization of assembly intermediates of the mitochondrial small subunit (mtSSU) in eukaryotes. To gain mechanistic insight into the process of SSU assembly in the …
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eL15 and eL22 proteins and their functional environments during ribosome assembly in Saccharomyces cerevisiae
… small nucleolar RNAs (snoRNAs) and more than 300 assembly protein factors, which are not present in the mature subunits and therefore are also known as trans-acting factors. The synthesis of ribosomes is a sequential process highly regulated and organized that starts in the nucleolus and orderly …
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Protein Factors Regulating Mitochondrial Respiratory Supercomplexes
… called supercomplexes (ETC SCs). Protein factors regulating ETC SCs remain largely unknown despite their fundamental implications to mitochondrial respiratory function. Recent knock-out studies have delineated external ETC proteins HIGD1A and HIGD2A as assembly factors of ETC complexes III …
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Molecular Physiology of the Chlamydomonas Pyrenoid
… of Rubisco aimed to identify key pyrenoid assembly factors. The present study combined and developed a wide range of molecular, physiological and computational techniques, to show that Rubisco aggregation forming the pyrenoid is achieved through a complex network of protein interactions in …
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Effect of experimental complex III deficiency on respiratory chain assembly and function
The assembly of respiratory chain complexes in the mitochondrial inner membrane requires specific factors. Once assembled to form a mature functional complex, complex III (CIII) is a dimer consisting of two monomers, each with eleven subunits. To date, seven assembly factors for CIII are known, of …
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Characterizing Factors That Influence Intracellular Thiol-Disulfide Equilibrium
… part of this thesis focuses on understanding the factors that influence the mitochondrial GSH:GSSG balance by using genetically engineered in vivo fluorescence redox sensors, rxYFP and roGFP2 (yellow and green fluorescence proteins). This project focuses on how specific oxidoreductases and their …
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Mechanism of Human Papillomavirus E6-Mediated Repression of P53-Target Gene *Transcription
… templates reconstituted with purified chromatin assembly factors (Acf1 and ISWI), core histones (H2A, H2B, H3, and H4), and a histone chaperone (NAP-1) in order to define the mechanism used by high-risk and low-risk HPV E6 to inhibit p53-dependent transcription. We demonstrate that E6 does not …
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Mass Spectrometry-Based Characterization Of Chloroplast Protein Complexes And Clp Protease Function
… the essential contribution of ClpP3 in Clp core assembly and function, as well as the crucial role of the Clp core complex in chloroplast viability. Large-scale, label-free quantification was used to characterize large (>800 kDa) soluble, chloroplast-localized protein and nucleoprotein assemblies …
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CHARACTERISATION OF A NOVEL SPINDLE DOMAIN IN MAMMALIAN MEIOSIS
… structural integrity. PCM1 degradation and disassembly of aMTOCs disrupted spindle assembly and reduced the total amount of nucleated microtubules throughout meiosis. In the absence of the main microtubule nucleating aMTOCs, oocytes relied on the Ran GTPase activity to form a small bipolar …
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The Molecular Anatomy of Mitochondrial Disease: Identification and Characterisation of Novel Nuclear-Encoded Mitochondrial Disease Genes
… putative cytochrome c oxidase (Complex IV, COX) assembly factor, and homozygous recessive sequence variants in TMCO6, which encodes an uncharacterised protein, were identified in patients presenting with classical clinical and biochemical hallmarks of mitochondrial disease. The aim of this work …
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The role of RNA modifications in mitochondrial translation
… of mitochondrial translation and/or ribosome assembly. The mitochondrial methyltransferase MRM2 modifies the human 16S mt-rRNA by introducing a 2’-O-methyl moiety in the ribose of residue U1369 (Um1369). This nucleotide is located in the aminoacyl site (A-loop of 16S mt-rRNA) of the …
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Investigating the role of R2TP-like co-chaperone complexes during axonemal dynein assembly
… proteins including chaperones, dynein axonemal assembly factors (DNAAFs), microtubule inner proteins (MIPs), the outer arm docking complex (ODA-DC) and the nexin-dynein regulatory complex (N-DRC). Chaperones work with co-chaperones to regulate their many functions within the cell. One of these …
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Elucidation of the Functional Architecture of the Early Pre-Ribosomal Processing Machinery in Yeast
… its immensely complicated and energy-consuming assembly pathway. The maturation of a eukaryotic ribosome involves more than 200 non-ribosomal factors and the activity of all three RNA polymerases. In yeast, ribosome biogenesis starts with the transcription of the 35S pre-ribosomal RNA in the …
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Assembly of cytochrome c oxidase: the role of hSco1p and hSco2p
… of the enzyme structure and the multiple factors and many steps required for its assembly. A functional COX requires three mitochondrially encoded subunits (Cox1p, Cox2p and Cox3p), at least 10 nuclearly encoded subunits, some of which are tissue specific, and a yet unknown number of …
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Biogenesis and Function of the Mitochondrial Ribosome
… one mitoribosomal particle. Proper function and assembly of this molecular machine is reliant on numerous associated factors, such as RNA modifying enzymes and assembly factors. Although recent significant progress has been made in our understanding of how the mitoribosome assembles and its …