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Showing 1 to 20 of 4672 for “"assays"”.
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Three Gastrointestinal Assays
… or from idiopathic symptoms. GI transit assays help to determine appropriate treatments but idiopathic symptoms, or pain from an unknown cause, is very common. The zebrafish is an attractive model system for human GI motility because the entire GI tract can be observed in intact zebrafish …
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Assays for lycosyltransferases
… was the development of operationally simple assays for GTs and their application for the identification and characterisation of novel GT inhibitors. The <br/>main focus was on the bacterial GT, α1,4-galactosyltransferase C (LgtC) which transfers galactose from uridine-diphosphate-galactose to …
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An Evaluation of Two Interleukin-1 Assays
… was to evaluate two of these methods. The two assays used were based on the fact that T cells proliferate in the presence of IL-1 and plant lectins. These assays differed with respect to the source of the T cells: nylon wool purified or D10.G4.1 cell line. Methods involving the use of D10.G4.1 …
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Silicon field-effect sensors for biomolecular assays
System-level understanding of biological processes requires the development of novel biosensors capable of quantitative, real-time readout of molecular interactions. Label-free detection methods can minimize costs in time and resources by obviating preparatory steps necessary with label-based …
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Development of DNA detection assays using functionalised nanoparticles
The ability to detect DNA relating to a specific disease is at the forefront of current research in molecular diagnostics. The detection techniques utilised for such work need to afford excellent selectivity as well as sensitivity. Current methods commonly involve the use of fluorescence; however …
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STATISTICAL FOUNDATIONS OF SINGLE CELL OPEN CHROMATIN ASSAYS
Single cell genomic assays are emerging technologies that measure biomolecules in each individual cell. The ability to measure molecules at the single-cell level provides unique opportunities to disentangle complex biological processes. Powered by technological advancements and the ever-decreasing …
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Solid-phase combinatorial chemistry and scintillation proximity assays
… in the detection and quantification of enzyme assays conducted within the matrix of the POP-Sc support.
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Sperm-egg interaction in birds: assays and mechanisms
… linearly correlated with standard sperm quality assays. The number of holes formed in the IPVL <i>in vitro</i> also correlated linearly with the number of holes formed in the IPVL of eggs fertilized <i>in vivo</i>, in inseminated hens; and logarithmically with the percentage of fertile eggs laid. …
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High sensitivity pathogen detection assays from crude samples
… we developed highly sensitive RT-LAMP based assays that can detect low copies of SARS-CoV-2 pathogen. We challenged ourselves to demonstrate even single-molecule detection of SARS-CoV-2 using a modified 2-step RT-LAMP process where only 2 primers are added during cDNA synthesis such that …
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Microfluidics for Cell Culture and Single Cell Assays
Elucidating molecular processes that govern cell function is paramount in forming a basis for understanding human health and treating disease. Research is presently limited by investigating in bulk, where information on critical subsets of cells is lost through averaging across an entire …
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Hydrogel-based microfluidic assays for multiplexed medical diagnostics
There is high demand for next-generation biomolecule detection platforms arising from clinical need for more robust point-of-care diagnosis systems, fundamental research need to characterize complex biological processes, and the emergence of personalized medicine. These detection systems should …
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Miniature bioanalytical assays utilizing mechanical actuation of microspheres
… necessary for gold standard bioanalytical assays. In such settings, lateral flow affinity assays remain the technique of choice, due to their fast time-to-result, ease-of-use and deliverable geometry. However, these assays suffer in sensitivity, with limits of detection 10 - 1000x higher …
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KINESIN-MICROTUBULE INTERACTIONS DURING GLIDING ASSAYS UNDER MAGNETIC FORCE
Conventional kinesin is a motor protein capable of converting the chemical energy of ATP into mechanical work. In the cell, this is used to actively transport vesicles through the intracellular matrix. The relationship between the velocity of a single kinesin, as it works against an increasing …
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Integrative Biomarker Identification and Classification Using High Throughput Assays
… as well as next generation sequencing assays interrogating somatic mutation, insertion, deletion, translocation and structural rearrangements. Given the massive amount of data, a major challenge is to integrate information from multiple sources and formulate testable hypotheses.</p> …
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DNA diagnostic assays using Surface Enhanced Raman Scattering (SERS)
… of target DNA sequences. Two novel DNA detection assays were also investigated which utilised SERS to determine the presence of sequences relating to the methicillin resistant Staphylococcus aureus (MRSA) strain. A solution based detection method was developed through coupling a TaqMan assay with …
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Readout of diagnostic assays on a centrifugal microfluidic platform
… microfluidic platform for multiplexed immunoassays <br>in the field of point-of-care applications. Multiplexing of immunoassays <br>is here defined as the detection of a set of different antigen <br>species from a given sample in a single channel. <br>Mixing as one of the crucial microfluidic …
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Liquid-on-liquid mixing for slide-based biological assays
Many parallel biochemical assays rely on thin aqueous films to spread a reactant solution over a wide area decorated with multiple distinct substrates. In this asymmetric, microfluidic geometry, diffusion limits the transport of reactants to substrates. Chemical equilibrium, a requirement for …
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Development of Assays for Neuronal Peptide Content and Release
Neuropeptide release is also studied with MALDI TOF MS. In this method, a small chamber (10 $\mu$l) is used to hold an isolated bag cell cluster in a small volume of physiological saline. The chamber consists of a standard pipette tip with the narrow end melted around two sections of fused silica …
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