Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 45 for “"assay development"”.
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Assay Development for Tau-directed Therapeutics
… induce disease-relevant aggregates and develop assays to screen therapeutics, this study explores mechanical shaking-induced aggregation and RNA-induced aggregation, as well as the effect of a commonly observed post-translational modification in NFTs, hyperphosphorylation, on tau aggregation. We …
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Assay development for use in drug discovery against Bovine Trichomoniasis
… this study was to develop new compound screening assays that will make the discovery of new compounds faster and more accurate. The CellTiter-Glo Luminescent Cell Viability Assay, a high throughput screening (HTS) assay from Promega, was found to be as affective at measuring cytotoxicity as …
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A rapid, simple solid-phase immunofluorometric assay : development and characterization
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Applied Biological Sciences, 1991.
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Applications of Engineered Proteins in Redox Biology and Biomarker Detection Assay Development
… very versatile tools that have been applied in assay development for various purposes. They have been made into genetically encoded biosensors/probes or affinity agents for biomarker detection. This thesis explored a few topics using assays developed with engineered proteins. The genetically …
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In vitro studies on genotoxicity and gene expression in spermatogenic cells: mechanisms and assay development
… is a complex process of male germ cell development from diploid spermatogonia to haploid fertile spermatozoa. Apoptosis plays a vital role in limiting cell numbers and eliminating defective germ cells. This requires novel gene products, and precise and well-coordinated programmes of gene …
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Biosensor and assay development for the detection of disease biomarkers at the point of care
Starting with the advent of biosensors in the late 1950s and early 1960s, biosensors have come to play a major role in various aspects of human life, including our environment, food and drink, safety, and health. In particular, the detection of clinically relevant biomarkers for the screening, …
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Detecting Dermacentor albipictus, the winter tick, at the northern extent of its range: Hunter-based monitoring and serological assay development
… by local hunters, to investigate growth and development of winter ticks on captive reindeer, and to develop a serological assay to detect antibodies to ticks using cattle as a model. Winter ticks were confirmed in 5 of 30 moose at 66° N. The development of ticks on captive reindeer was …
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MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE SIMULTANEOUS DETECTION AND IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS AUREUS
… Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect <em>S. aureus</em> and <em>S. pneumoniae</em> in the same sample.</p>
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Point of care platforms for protein and nucleic acid detection
… using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding. A multiplexed microbead immunoassay is developed and validated first for the detection of IL-6. The assay principles are further used to develop microbead based detection for PCT. The final platform …
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DEVELOPMENT OF SURFACE BASED PLATFORMS FOR BIOMOLECULAR RECOGNITION AND PROTEASE ASSAYS
… this thesis we present novel approaches for the development of protease activity assays. Our focus of study is the protease assay development process on two types of characterized platforms: microspheres and self-assembled monolayers (SAMs) on flat surfaces. The surface preparation and …
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Microfluidic devices for studying early response of cytokine signaling
… towards an efficient and productive cell-based assay development. In particular, this thesis demonstrates the feasibility of culturing human cancer cells in microliter-volume reactors in batch and fed-batch operations, stimulating the cell under well-controlled and reproducible conditions at …
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Development of highly specific monoclonal antibodies to Listeria sp. and Listeria monocytogenes
… spp., a selective culture broth, and an immunoassay to enable a 21 hour assay to detect Listeria spp. in foodstuffs. Listeria is a common contaminant of food production. Its hardiness and ability to grow under refrigeration make it a significant contaminant of pre-cooked foods and processed …
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Early-stage drug discovery of HIV-1 frameshifting modulators
… of HIV-1 Gag:Gag-Pol frameshifting. Following assay development and validation of screening parameters, a primary diversity screen with 92,071 small molecules was conducted. The initial screening triage of hit compounds led to the selection of several false positives due to auto-fluorescent …
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Development of biophysical and Mass Spectrometry Assays for APOBEC3 cytosine deaminases: discovery and validation of cnvalent and non-covalent ligands
… genomic instability, tumor heterogenity, and the development of therapeutic resistance. APOBEC3 proteins are therefore important drug targets for the development of inhibitors that can be used in combination with traditional cancer therapies to increase their efficacy. This thesis will discuss …
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The Development of Assays for Detecting Proximal Phosphorylation and Covalent Modification of Proteins
Bioassays are important tools in translational research for several disciplines including medicinal chemistry, molecular recognition and chemical biology. Achieving success in bioassays represents important milestones in many pharmaceutical pipelines and disease diagnostics, and as such, must be …
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The Development of Substrates and Inhibitors of the PNAG Biosynthetic Machinery
… of the PNAG polysaccharide. An NMR-based assay was developed to more directly observe the rates of PNAG biosynthesis in vitro. Analogues of UDP-GlcNAc incorporating NMR-active nuclei were synthesized using a chemoenzymatic approach and validated with the processive glycosyltransferase …
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NONHUMAN PRIMATES AS A TRANSLATIONAL MODEL OF ENDOMETRIOSIS AND ITS EFFECTS ON WOMEN'S HEALTH
… NHP archived tissue, an in vitro cell culture assay, and RT-qPCR. Endometrium and endometriosis tissue metabolism was analyzed using isolated mitochondrial respirometric analysis and targeted metabolomics. The effects of endometriosis on systemic iron metabolism were identified using NHP and …
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Chemical Biology Tools to Study Bacterial Cell Surface Glycans
… important downstream applications, including assay development and controlled cell surface perturbation (Chapter 3). We then demonstrated the generalizability of this approach by developing probes for mannose-containing glycans using this strategy (Chapter 4). In this work, we have also …
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Application of process analysis and optimization tools in hit-to-lead and lead optimization phases of drug discovery at EPP, NIBR
… increment in personnel scientific knowledge, development of new analytical software and/or changing the areas of study. After realizing the limited results achieved with these approaches, lab representatives started to notice the opportunity of introducing process optimization tools, such as …
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Molecular characterization of the major envelope protein of porcine reproductive and respiratory syndrome virus (PRRSV) and evaluation of its use for a diagnostic assay, vaccine development, and the examination of quasispecies evolution
… of PRRSV through genetic characterization, assay development, and quasispecies evaluation using the PRRSV ORF5 gene while also attempting to develop an improved PRRS vaccine. To explore the possible mechanism for the emergence of acute PRRS, the open reading frame 5 (ORF5) gene encoding the …
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