Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"artificial chromosomes"”.
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Human common fragile sites, identification, and analysis of breaks using <i>saccharomyces cerevisiae</i>
… mapped 30 break locations in each of two yeast artificial chromosomes (YACs) containing human DNA inserts from fragile sites FRA3B and FRA7H. We compared break locations with the locations of high flexibility regions and potential origins. Breaks in both FRA3B and FRA7H are located far from …
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Physical Map between Marker 8O7 and 146O17 on the Medicago truncatula Linkage Group 1 that Contains the NIP Gene
… the model legume Medicago truncatula bacterial artificial chromosomes (BACs) were used to create a physical map on the DNA in this genetic internal. BACs near the potential NIP gene location near marker 164N9 were identified, and used in experiments to predict the physical map by a BAC-by-BAC …
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Creating a versatile toolkit for transgene expression using BACS and for dissecting large-scale chromatin organization
… complexity of the mammalian genome. Bacterial artificial chromosomes (BACs), containing 100-300 kb mammalian genomic regions have been shown to recapitulate the expression level and nuclear localization of the corresponding genomic regions, and to protect embedded reporter mini-genes from …
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Palindromes on the human X chromosome : testis-biased transcription, gene conversion and evolution
… X chromosome palindromes. We sequenced bacterial artificial chromosomes (BACs) from chimpanzee, orangutan and rhesus monkey genomic libraries containing sequence orthologous to several of the human X chromosome palindromes. We found some of the palindromes conserved in all species
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Development of embryonic stem cells expressing endogenous levels of a fluorescent protein fused to the telomere binding protein TRF1
… associated proteins found at the ends of linear chromosomes. They have a role in biological processes including meiosis and aging as well as implications in a number of genomic instability disorders and cancers. Telomeres maintain genomic stability by protecting chromosome ends from terminal …
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Positionsklonierung des Morbus Best-Gens VMD2
… aus artifiziellen Phagenchromosomen ('phage artificial chromosomes', PAC) erstellt, der die VMD2-Kandidatengenregion auf Chromosom 11q12-q13.1 physikalisch repräsentiert. Durch die Identifizierung polymorpher (CA)n-Dinukleotidmarker aus dem kritschen Intervall und anschließender …
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An in vitro chemogenomic screening system to identify the molecular targets of antimalarial drug candidates
… I leveraged an existing library of *P. berghei* artificial chromosomes (PbACs), of which ~600 containing potential drug targets (transporters, enzymes, metabolic pathways) were engineered to possess a unique DNA barcode. These barcodes can be used to detect and quantify constructs within complex …
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Analyses of hematopoiesis and lineage commitment from ES and iPS cells
… Reporter-ES-Zelllinien generiert, die bacterial artificial chromosomes (BACs) tragen, welche YFP unter Kontrolle des preTα-Promotors und GFP unter Kontrolle des λ5-Promotors exprimieren. Die Analyse der in vitro-Differenzierung zu B- und T-lymphoiden Zellen zeigte, dass BAC λ5-GFP-transgene …
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Physical mapping of a 2-Mb region centered at D10S94, a locus very tightly linked to the multiple endocrine neoplasia type 2 gene(s)
… were present at DlOS94 in these patients. Yeast artificial chromosomes (YACs) identified in collaboration with Drs. Ken Kidd and Jay Lichter, expanded DlOS94 and, with genomic long range restriction mapping, established physical linkage to six other DNA markers near MEN2. Recent published reports …
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Engineering Escherichia coli as a general genome synthesis platform
… DNA (1–10 kb) pieces are assembled to bacterial artificial chromosomes (BACs, 50–100 kb), which are then used to replace wildtype sequence in the E. coli genome. Conjugation coupled with programmed excision for enhanced recombination (CONEXER) accelerates the E. coli genome synthesis workflow. …
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Common Alleles of the SLAM/CD2 Family are Associated with Murine Lupus
… 172.2 Mb. A contig of 100 B6-derived Bacterial Artificial Chromosomes (BACs) was constructed across Sle1b, and sequencing of six BACs that form an overlapping tiling path across it revealed that the interval contains 24 genes, 19 of which are expressed in the spleen, and 14 of which are in B and …
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Molecular analysis of sex chromosome evolution in papaya
Sex chromosomes are of great interest due to their role in sexual reproduction. Sex chromosomes evolve through the suppression of recombination in the sex determining region between the X and Y chromosomes which allows for each region to evolve and change independently. Papaya’s trioecious sex is …
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Elektroporation von Säugerzellen
Ziel der Arbeit war es, die wissenschaftlichen und technischen Voraussetzungen zu schaffen, um eine effiziente Elektroporation von großen aber auch kleinen Zellzahlen zu erreichen. Ein großer Teil der Arbeit diente der Entwicklung eines neuen Elektroporationsgerätes, des Multiporators. Die …