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Showing 1 to 20 of 31 for “"aromatic amino acids"”.
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Genetic and phenotypic analyses of auxotrophs requiring aromatic amino acids and vitamins in Streptomyces coelicolor
… coelicolor having auxotrophic requirement for aromatic amino acids were mapped relative to closely linked genetic markers. Based on four-point reciprocal crosses mutants were found to map in at lea.st two locations. One mutant mapped between proA and hisC while three fell between argA and hisE. …
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The tyrosine regulated DAHP synthase and the biosynthetic pathway of aromatic amino acids in Saccharomyces cerevisiae
… aus S. cerevisiae sind vorhanden. Mithilfe von Aminosäuresubstitutionen am katalytischen Zentrum (Lys112Ala, Arg114Ala and Arg180Ala) konnte die Bedeutung der für die Katalyse wichtigen Reste der Loops L2 und L4 gezeigt werden.Die Strukturelemente, die wichtig für die Katalyse sind, …
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Metalloprotein Engineering Using Heme Protein Scaffolds to Investigate the Oxidation of Endogenous Aromatic Amino Acids and Exogenous Substrates
… these enzymes. The role of role of redox active amino acids in heme proteins was studied by mutating Trp an Tyr residues in cytochrome c peroxidase (CcP). Sequential mutation of these residues resulting in increased lifetime of the compound I intermediate. An Engineered Trp in the active site of …
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Aromatic Amino Acid Metabolome In The Human Gut Microbiota
… molecules are simple structures derived from aromatic amino acids (phenylalanine, tryptophan, and tyrosine). In this thesis, I employed targeted and untargeted mass spectrometry-based approaches to identify novel bacterial metabolites that derived from aromatic amino acids. In chapter 2, I …
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Velocity-difference induced focusing in capillary electrophoresis and preparative capillary electrophoresis
… detector. It was also applied to focus amino acids, peptides, and proteins to improve the concentration sensitivity. It is found that the pKa rather than the pI of the analytes is the key criterion for selecting the pH for the sample and for the BGE to obtain the optimum focusing for …
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Characterization of the Pathway Leading to the Synthesis of Salicylic Acid in Plants Resisting Pathogen Infection.
… as a common substrate with the synthesis of aromatic amino acids, HPLC was used to determine whether the increase in SA could be attributed to a decrease in amino acid levels. The results suggest that genes of the shikimate pathway are up-regulated in both plant lines but much more quickly in …
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Photolyase: Its Damaged DNA Substrate and Amino Acid Radical Formation During Photorepair
… photolyase containing isotopically-labeled aromatic amino acids, we observed that tryptophan residues in photolyase underwent structural changes during photorepair. These data indicate that electron transfer during DNA repair occurs through-bond, and that redox-active, aromatic residues form …
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Experimental and Computational Studies in Bioorganic and Synthetic Organic Chemistry
… and function. Among the three proteinogenic aromatic amino acids, tryptophan (Trp) is the strongest cationâ Ï donor. We reported the asymmetric syntheses of tryptophan regioisomers in which the amino acid side chain is attached at different position of the indole moiety. These new tryptophan …
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Identification and Characterization of a Suppressor Mutation That Relieves the Enzymic and Growth Defects of Sod-Deficient Escherichia Coli
… for sulfur-containing, branched-chain, and aromatic amino acids and cannot catabolize non-fermentable carbon sources. A secondary-site mutation substantially relieved these growth defects. This suppression was mediated at least in part by the restoration of activity to the oxidatively labile …
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Integration of metabolic modelling with machine learning to identify mechanisms underlying antibiotic killing
… efficacy. Additionally, roles for metabolism of aromatic amino acids and biosynthesis of polyprenoids were identified as pathways meriting further investigation.
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Plant aromatic amino acid decarboxylases: Evolutionary divergence, physiological function, structure function relationships and biochemical properties
Plant aromatic amino acid decarboxylases (AAADs) are a group of economically important enzymes categorically joined through their pyridoxal 5'-phosphate (PLP) dependence and sequence homology. Extensive evolutionary divergence of this enzyme family has resulted in a selection of enzymes with …
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ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΚΑΘΑΡΙΣΜΟΣ ΤΟΥ ΕΝΖΥΜΟΥ L-DOPA ΑΠΟΚΑΡΒΟΞΥΛΑΣΗ ΑΠΟ ΤΙΣ ΛΕΥΚΕΣ ΝΥΜΦΕΣ ΤΟΥ ΕΝΤΟΜΟΥ CERATITIS CAPITATA ΚΑΙ ΑΠΟ ΑΝΘΡΩΠΙΝΟ ΝΕΦΡΙΚΟ ΙΣΤΟ. ΣΥΓΚΡ...
… ENZYME CATALYZING THE DECARBOXYLATION OF CERTAIN AROMATIC AMINO ACIDS TO THEIR RESPECTIVE AMINES. SINCE DOPAMINE IS INVOLVED IN MANY IMPORTANT METABOLIC PROCESSES PLAYING A CENTRAL ROLE IN THE PATHOGENESIS OF CERTAIN NEUROLOGICAL DISORDERS AND ON THE OTHER HAND O-DIPHENOLIC SUBSTANCES SUCH AS …
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Isolation and characterization of channel-forming proteins in the outer membrane of E. coli and Borrelia species
… 3D-structure of LamB suggests that a number of aromatic residues (Y6, Y41, W74, F229, W358 and W420) within the channel lumen is involved in carbohydrate and ion transport. All aromatic residues were replaced by alanine (A) scanning mutagenesis. Furthermore, LamB mutants were created in which …
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Multidomain Peptides: Sequence-Nanostructure Relationships and Biological Applications
… in which the A block is composed of charged amino acids, such as lysine, and the B block consists of alternating hydrophilic and hydrophobic amino acids, such as glutamine and leucine. The B block forms a facial amphiphile that drives self-assembly. The charged A blocks simultaneously limit …
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Methanocaldococcus jannaschii and the Recycling of S-adenosyl-L-methionine
… the production methylglyoxal, a precursor for aromatic amino acids. MTIP and MTRI were demonstrated to produce 5-deoxyribulose 1-phosphate from 5'-dI. Additionally, two enzymes annotated as part of the pentose phosphate pathway, ribulose 5-phosphate 3-epimerase and transketolase, were able to …
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Exploring the function and sensing mechanisms of human enteroendocrine cells using intestinal organoids
… response to glucose. Furthermore, we knocked-out amino acid-sensing GPCRs CaSR and GPR142 by CRISPR-Cas9 NHEJ, demonstrating that both are needed for the release of GIP from organoid-derived human K-cells. Amino acid sensing and FFAR1-mediated fat sensing by K- and L-cells were further …
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Detection of Proteins by Two-Photon Excitation of Native Fluorescence
… is based on excitation of native fluorescence of aromatic amino acids by simultaneous absorption of two photons. First, a compact two-photon prototype detector was investigated. Its sensitivity was improved after discovery of the source of the background and subsequent reduction of background …
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Comparative studies on the modes of action of SC-0224 and glyphosate
… SC-0224 (trimethylsulfonium carboxymethylaminomethylphosphonate) and glyphosate [N-(phosphonomethyl)glycine] (PMG) were compared. In each study trimethylsulfonium iodide (TMS-I) was included as a treatment because the trimethylsulfonium ion is a constituent of the SC-0224 molecular …
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Investigating Karyopherin B1: small molecule interactions for cancer therapy
… occurring in a region of KpnB1 containing aromatic amino acids. The purified GST-KpnB1 was used in ITC, which confirmed an interaction between KpnB1 and INI-43, a relatively weak interaction. In conclusion, our data shows that the small molecule, INI-43 kills cancer cells, likely by …
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Electrostatic properties at the interface of p53 Transactivation domain binding
… CBP or MDM2. We observed that aliphatic and aromatic amino acids experienced the largest relative change in sidechain dipole moments, and that there is sensitivity to binding shown in this dipole response. The largest percent changes in sidechain dipole moment were found to localize at and …
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