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Showing 1 to 16 of 16 for “"arginine deiminase"”.
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Kinetic Characterization of the Protein Arginine Deiminase Family: Mechanistic and Inhibition Studies
<p>The family of Protein Arginine Deiminases (PADs) include five mammalian isozymes (PAD1, 2, 3, 4, and 6) that catalyze the post-traslational modification of peptidyl-arginine to peptidyl-citrulline in numerous protein substrates. This modification results in numerous physiological and …
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Gene Knockout Of PRFA And The Arginine Deiminase Pathway In Listeria Monocytogenes
… which are involved in the disease process. The arginine deiminase pathway has the ability to regulate acidic environments by converting arginine to ammonia, ornithine, and ATP. With the help of a membrane-bound antiporter, the ornithine is transported out of the cell while the ammonia is …
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Unlocking thePadlock: Elucidation of Protein Arginine Deiminase 4 Function, Activity and Activation Dynamics
The Protein Arginine Deiminase (PAD) family contains five known mammalian isozoymes (PADs 1, 2, 3, 4, and 6) that catalyze the post-translational modification of peptidyl-arginine to form peptidyl-citrulline in a variety of protein substrates. Over the past decade the importance of this post …
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Characterising the Role of the Calcium-dependent Citrullinating Enzyme Peptidyl Arginine Deiminase 2 in Ovarian Cancer
… The dysregulation of calcium-dependent peptidyl arginine deiminase 2 (PADI2) plays a key role in the tumorigenesis of several cancers; however, the role of PADI2 in the pathogenesis of EOC is yet to be investigated. Using RNA-seq and microarray data from primary serous ovarian cancers (The Cancer …
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Vitamin D Inhibits Expression of Protein Arginine Deiminase 2 and 4 in Experimental Autoimmune Encephalomoyelitis Model Of Multiple Sclerosis
… collection of symptoms. Two enzymes, protein arginine deaminases type 2 and 4 (PAD2 and PAD4) have been implicated to play an etiologic role in demyelination and neurodegeneration by catalyzing a post-translational modification of arginine peptide residues to citrulline. The pathogenesis of MS …
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Arginase from Bacillus caldovelox
… and other thermophilic bacteria were cultured in arginine-supplemented media and screened for arginase, arginine deiminase and arginine decarboxylase activity by a reversed-phase liquid chromatography method developed to detect these arginine-catabolizing enzymes in cell-free extracts. Thermus, …
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Discovery and characterization of ribosomally synthesized and post-translationally modified peptide natural products
… and exceedingly rare. Surprisingly, the peptidyl arginine deiminase responsible for the modification of arginine to citrulline in bacteria is not known. Comparative genomics was employed to find a putative PAD enzyme, which was subsequently heterologously expressed and tested for arginine …
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Inhibition of extracellular vesicle release enhances cancer chemotherapy and inhibition of outer membrane vesicle release sensitises bacteria to antibiotic and bacteriophage therapy
… minimal effect on the cell viability. Peptidyl Arginine Deiminase (PAD) inhibitor, Chloramidine (Cl-amidine, Cl-am) and Protein Kinase C inhibitor, Bisindolylmaleimide-I (Bis-I) exhibited extensive EV inhibition in PC3 and MCF-7 cells. This effect was further tested using an Annexin-V-based …
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Kinetic, Mechanistic, and Inhibition Studies On the Deiminase Family of Enzymes
<p>Protein arginine deiminase (PAD) and agmatine deiminase (AgD) both belong to the guanidino-group modifying enzymes superfamily. Although these two enzymes catalyze a similar reaction, the conversion of a guanidinium to a ureido and ammonia, their substrates differ greatly, i.e., …
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Development of Novel Small-Molecule Therapeutics for Multiple Sclerosis
Protein arginine deiminase (PAD) activity is upregulated in MS and the overactive enzymes convert peptidyl arginine on myelin structural proteins to neutral citrulline variants. Hypercitrullination disrupts key ionic interactions between myelin proteins and lipid bilayers that are necessary to …
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Synthesis of functionalised sulfonamides
… evaluation against the enzymes dimethylarginine dimethylamino hydrolase (DDAH) and arginine deiminase (ADI). Several compounds displayed \muM inhibition against DDAH and ADI; and in addition, these currently represent the first known inhibitors of ADI. During the course of our …
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Characterisation of HP1γ in mammalian cells
… these residues are citrullinated by peptidyl arginine deiminase 4 (PADI4) in vitro and in vivo. Mutations in HP1γ (R38/9A), designed to mimic the loss of charge accompanied with citrullination, affect HP1γ’s binding to H3K9me3 peptides and reduce its residence time on chromatin in …
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Identificación taxonómica y clonal de bacterias acéticas, y estudio del efecto de la nisina frente a biofilms de bacterias enológicas
… it was unveiled that this LAB utilizes the ADI (arginine deiminase) pathway of arginine degradation as mechanism of response to the presence of ethanol. It generated ornithine and ammonium, but it did not generate any biogenic amine (agmatine, spermidine, phenylethylamine, histamine, or …
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Characterization of the Factors Associated with SCCmec Mobility in Staphylococcus Aureus
… type IV and a genomic island containing an arginine deiminase pathway, known as ACME, inserted adjacent to one another in the SCCmec chromosomal attachment site. Each element was site-specifically excised from the chromosome by CcrAB, resulting in two independent, extra-chromosomal, …
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Biomolecular NMR studies of the structure and biosynthesis of RiPP natural products
… and complementation experiments identified the arginine deiminase responsible for the installation of this functional groups. Solution NMR analysis generated a 3D solution NMR ensemble structure for this peptide. In Chapter 6 we report the discovery of two novel thiopeptide RiPPs. Genome-mining …
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Proteomics: A close encounter with rheumatology
… Citrullinatie, een modificatie waarbij een arginine omgezet wordt tot citrulline, veroorzaakt een shift op een 2-D beeld naar de zure kant en bovendien is gecitrullineerd vimentine een gekend autoantigen in RA. Om deze redenen werd de mogelijke aanwezigheid van deze modificatie onderzocht. …