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Showing 1 to 20 of 110 for “"aptamers"”.
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Aptamers as biosensors
Aptamers are short, single strands of artificial nucleic acids which bind to specific targets. This binding is similar to the binding exhibited by antibodies but aptamers offer improved properties and consequently are replacing antibodies in some applications. This first part of this project …
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Optimizing Acyclic Identification of Aptamers
… m=15, with the High Throughput Screening of Aptamers (HTSA) technique, later termed Acyclic Identification of Aptamers (AIA). AIA eliminated the need for multiple cycles of in vitro evolution typically used for aptamer discovery by employing libraries with an over-representation of all …
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High Throughput Screening of Aptamers
<p>Aptamers are functional oligonucleotides that are discovered through directed evolution. With affinities for their targets similar to antibodies, aptamers are useful in biosensors, diagnostics and therapeutics. Three elements of natural selection--variation, selection, and replication afford …
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Functional DNA Aptamers as Biotherapeutic Molecules
Aptamers are single-stranded oligonucleotides, DNA or RNA, which can bind to a myriad of targets such as ions, peptides, proteins, drugs, organic and inorganic molecules with high affinity and specificity. Aptamers are derived using combinatorial libraries comprised of a variable region flanked by …
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Microfluidic Selection and Applications of Aptamers
… reducing the time and reagents necessary to find aptamers. In particular, microscale "selection" or partitioning of weakly bound sequences from aptamer candidates, and on-chip integration of the protocol have separately been explored as approaches to scaling and improving SELEX. Initial results …
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Selecting DNA aptamers for 17β-estradiol
… promising approach which employs the use of DNA aptamers for the detection of E2. DNA aptamers are single stranded DNA molecules which are capable of binding target molecules with high affinity and selectivity. The aim of this project is to develop DNA aptamers that would function as sensors for …
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SELECTION OF APTAMERS FOR INTESTINAL PERMEABILITY TEST.
… method to evolve nucleic acid ligands, called aptamers, with new functionalities. We have prepared single-stranded DNA aptamers against non-metabolizable sugars and peptide using SELEX, with the ultimate goal of developing biosensors for the intestinal permeability test. Design strategy for …
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Selective targeting to glioma with nucleic acid aptamers
… is difficult, and patient survival is poor. Aptamers are small molecular ligands composed of short oligonucleotides that bind to a target with high specificity and affinity. They are produced in vitro through a method called systematic evolution of ligands by exponential enrichment (SELEX). …
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The Identification and Validation of Novel Aptamers to Glioma
… to deliver therapeutics more specifically. Aptamers are short oligonucleotides that conform to unique three dimensional structures based on sequence and are able to target molecules selectively, in order to deliver therapeutic agents directly to the tumour. Aptamers can be selected against …
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USE OF DNA APTAMERS FOR TARGETED DELIVERY OF CHEMOTHERAPEUTIC AGENTS
… to large biomolecules such as antibodies. Aptamers are a new class of biomolecules similar to antibodies in that they fold into 3D shapes which specifically bind to a target of interest, but differ in that they are composed of single stranded RNA or DNA. Aptamers are selected for using the …
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The Interrogation of Cas9 Aptamers and sgRNA Structures Through SELEX
… assay was utilized to identify modified RNA aptamers that bind to Cas9 with specificity and an affinity rivaling that of the sgRNA. The aptamer was shown to tolerate truncations and sequence additions, demonstrating an ability to localize oligonucleotide-based therapeutics to the Cas9 …
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Fluorescent and Colorimetric Biosensors Based on DNAzymes and DNA Aptamers
… a large number of catalytic DNA (DNAzymes) and aptamers have been isolated, many of which possess high analyte specificity. As a result, these functional nucleic acids can be used for sensing and diagnostic applications. However, the number of successful sensors made from these nucleic acids is …
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Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis
… becomes crucial in controlling EPTB. Aptamers referred to as "synthetic antibodies" have been recently explored as a replacement for antibodies in diagnostic platforms. These single-stranded nucleic acid molecules have high affinity and specificity comparable, and in some instances …
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Comparing Anti-VEGF Antibodies and Aptamers on Paper Microfluidic-Based Platforms
… In the detection region both antibodies and aptamers were evaluated.</p> <p>The fiberglass pad where conjugate reagents would be contained, were initially saturated in dye. As sample was inputted into the three-dimensional chip, the fluid path could be visualized. Without the use of the …
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In vitro selection of aptamers using quantum dot-assisted capillary electrophoresis SELEX
<p>Aptamers are short, synthetic oligonucleotide sequences that can selectively bind to targets with high affinity. Selection of aptamers is accomplished by the Systematic Evolution of Ligands by EXponential enrichment (SELEX). The target is incubated with a random sequence oligonucleotide library, …
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A Microfluidic Approach to Selection and Enrichment of Aptamers for Biomolecules and Cells
… selection and amplification of nucleic acids (aptamers) that bind to specific targets. Aptamers are very attractive molecules in many biological applications due to their interesting properties including high target binding affinities and stability. Using conventional platforms for aptamer …
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The Generation of Novel Aptamers for Glioma and their Pharmacokinetic Profile In Vitro
… and eventual mortality inevitable. The use of aptamers has been postulated as a targeting mechanism for the selective treatment of cancer. Aptamers are single stranded, structured nucleic acid ligands with the capacity to specifically bind to target molecules. Aptamers were obtained through …
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Selection and characterization of DNA aptamers for estradiol and ethynylestradiol for aptasensor development
… A promising alternative makes use of DNA aptamers as molecular recognition elements. In order to evaluate the potential of DNA aptamers and aptasensors to detect small organics in natural waters, the following objectives were pursued: (1) critically review DNA aptamers and aptasensors …
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Identification of malaria parasite-infected red blood cell aptamers by inertial microfluidics SELEX
… the elaborate RBC surface milieu. Nucleic acid aptamers are widely used in biological applications for their high specificity and affinity to targets and are highly suitable for malaria applications. Efficiently generating aptamers against complex targets-such as whole cells-remains a challenge. …
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A computational approach for the discovery and characterization of oligonucleotide aptamers for protein targets
Aptamers are short polynucleotide chains that can bind with very high affinities to a broad range of chemical and biomolecular targets, including proteins, cells, viruses, microorganisms, toxins, and chemical compounds. They are analogous to antibodies with applications in therapeutic delivery, …
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