Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"apolipoprotein A"”.
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ROLE OF APOLIPOPROTEIN A-V IN TRIGLYCERIDE METABOLISM
… gene cluster on human chromosome 11q23. Apolipoprotein A-V (apoA-V) plasma concentrations are low (~100-200 µg/L) in comparison to other apolipoproteins, yet exert significant effects on triglyceride homeostasis. To examine the basis for its low concentration in plasma, the secretion …
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Transcriptional Regulation of Chicken Apolipoprotein A-I Gene Expression
… between v-Jun and c-Jun in CEF, the chicken apolipoprotein A-I (apoA-I) gene has been identified in our laboratory as one of the target genes whose expression is repressed in response to v-Jun overexpression in CEF. The overall objective of this study is to investigate the underlying …
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Interaction of Human Apolipoprotein a-I With Dipalmitoyl Phosphatidylcholine
… (DPPC) bilayer lipid will react with human apolipoprotein A-I (apo A-I) to form lipid-protein micellar complexes. The reactivity of the lipid bilayer is dependent upon the physical state of the vesicle. Large vesicles with essentially planar bilayers do not form complexes except at …
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The Role of Lipid-Free Apolipoprotein A-I and PCPE2 in HDL Metabolism
… of this work is to investigate the role of apolipoprotein apoA-I (apoA-I) and procollagen C-endopeptidase enhancer 2 (PCPE2) in high density lipoprotein (HDL) metabolism. ApoA-I comprises ~70 percent of the protein within HDL and has an essential role in cholesterol efflux from peripheral …
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The Role of Apolipoprotein A-I in the Modulation of Cholesterol and Immune Homeostasis
The goal of this work was to examine the role of apoA-I in atherosclerosis and autoimmunity. We used a mouse model lacking the LDL receptor and the apoA-I gene (LDLr-/-,ApoA-I-/- or DKO). LDLr-/- (SKO) mice were used as controls. When fed a cholesterol-containing diet, DKO mice exhibited …
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Structural Studies of Lipid-Bound Apolipoprotein A-I and Nanodisc-Embedded Cyp3A4 by SSNMR
Membrane proteins constitute more than half of all drug targets in the pharmaceutical industry today, which is not surprising given that a third of human genome codes for membrane proteins, and that they perform a large number and variety of cellular functions. However, membrane proteins to date …
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IMPACT OF HEPATIC APOLIPOPROTEIN A-IV EXPRESSION ON VLDL PARTICLE EXPANSION, TRIGLYCERIDE SECRETION, AND STEATOSIS
Several apolipoproteins (apo) can impact triglyceride (TG) transport by modulating VLDL assembly and secretion. Here we explore the impact of apoA-IV on VLDL particle expansion. Previous studies demonstrated that apoA-IV expression promotes apoB lipoprotein-mediated TG secretion in transfected …
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The Role of Lipoprotein(a)/Apolipoprotein(a) in Endothelial Dysfunction: Mechanistic Studies in Vascular Endothelium
… (LDL)-like moiety and an unique glycoprotein, apolipoprotein(a) (apo(a)), that is covalently attached to the apolipoproteinB-100 (apoB-100) component of LDL by a single disulfide bond. Many studies have suggested a role for Lp(a) in the process of endothelial dysfunction. Indeed, Lp(a) has been …
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Lipid acquisition by apolipoprotein A-I in ER and Golgi compartments of primary mouse hepatocytes
It was previously unknown what the significance and location of intracellular lipidation of newly synthesized apoA-I in hepatocytes were in plasma HDL formation. By labeling primary mouse hepatocytes with 3H-choline, we showed that phospholipidation of apoA-I is most significant in endoplasmic …
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Synthetic Micellar Complexes of Apolipoprotein a-I With Lipids as Substrates for Human Lecithin Cholesterol Acyltransferase
Micellar lipid-apolipoprotein A-I (apo A-I) complexes were produced by adding human apo A-I to lipid dispersions containing sodium cholate. The lipid mixtures included dimyristoyl phosphatidylcholine (DMPC), dipalmitoyl phosphatidylcholine (DPPC), and egg phosphatidylcholine (egg-PC). The formation …
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Oxidative Modifications of Apolipoprotein(a): Implications for Proinflammatory and Prothrombotic Roles of Lipoprotein(a) in the Vasculature
Elevated plasma concentrations of lipoprotein(a) (Lp(a)) have been identified as a causal risk factor for calcific aortic valve disease (CAVD) and coronary heart disease (CHD). Relationships have recently been identified for genetic factors, such as single nucleotide polymorphisms (SNPs) in the LPA …
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Structural and functional studies of the C-terminal domain of human apolipoprotein A-I: Limited proteolysis and deletion mutagenesis
… to produce a large N-terminal fragment of human apolipoprotein A-I (apoA-I). Digestion of reconstituted high density lipoprotein (rHDL) prepared with apoA-I and dipalmitoyl phosphatidylcholine (DPPC) or palmitoyloleoyl PC (POPC) by chymotrypsin, trypsin, elastase, or subtilisin generated a major …
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Molecular level characterisation of apolipoprotein A-I aggregation leading to fibrils comprising of both α-helical and β-sheet structures
Amyloidosis is defined as the misfolding of native proteins into insoluble fibrils that are deposited within tissues and extracellular organs. 30+ structurally and sequentially unrelated proteins have the ability to form amyloid aggregates, all of which contain characteristic features. ApoA-I, the …
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Studies of G-Protein Coupled Receptors Incorporated Into a Novel, Nanoscale, Membrane-Mimetic System
<p>From first principles of phospholipid — apolipoprotein A-I (apo A-I) interactions, we hypothesized that the amino acid sequence of apo A-I derived from a different species may exhibit improved properties compared to human apo A-I for the purpose of incorporation of G protein-coupled receptors …
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Factors affecting the binding of lecithin:cholesterol acyltransferase with interfacial substrates
… homeostasis. The principal protein of HDL, apolipoprotein A-I (apoA-I) is a major physiological activator of the LCAT reaction. Despite the importance of this reaction, the factors which influence LCAT affinity for the surface of HDL are poorly understood. To determine what properties of HDL …
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Molecular Basis of HDL-Mediated Endothelial Cell Migration and Reendothelialization
… perivascular electric injury is blunted in apolipoprotein A-I null (apoA-I-/-) mice, and reconstitution of apoA-I expression rescues normal reendothelialization. Furthermore, reendothelialization is impaired in SR-BI-/- mice. Thus, HDL stimulates endothelial cell migration via …
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Investigating the Role of Standing Blood Pressure Measurement and the Association Between High-Density Lipoprotein and Skeletal Muscle Mitochondrial Function in Pre-Hypertensive Humans
… demonstrate higher levels of HDL cholesterol and apolipoprotein A-I are independently associated with enhanced skeletal muscle mitochondrial function in humans. These findings are important because disruptions in skeletal muscle metabolism have been associated with exercise intolerance. In …
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ENGINEERING WATER-SOLUBLE VARIANTS OF THE SINGLE-SUBUNIT OLIGOSACCHARYLTRANSFERASE
… amphipathic protein including engineered human apolipoprotein A-I. Using E. coli culture as an expression platform, several SIMPLEx-OSTs could be expressed within the cytoplasmic fraction of the E. coli. Importantly, our engineered OSTs retain their biological activity and are able to …
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Spontaneous Phosphatidylcholine Exchange Between Vesicles and Micellar Lipid-Apolipoprotein Complexes
… model lipoproteins were recombinants of bovine apolipoprotein A-I, egg PC, and cholesterol produced by cholate dialysis. Vesicles contained egg PC and cholesterol in varying molar ratios. Lipid exchange was followed by incubating radiolabeled vesicles (('3)H-PC, plus ('14)C-cholesterol oleate as …
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