Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"aortic endothelial cells"”.
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Receptor-mediated endocytosis of low density lipoproteins in aortic endothelial cells
… and quiescent (postconfluent) bovine aortic endothelial cells (ECs) were qualitatively investigated by fluorescence microscopy using dioctadecylindocarbocyanine-labelled lipoproteins and by indirect immunofluorescence microscopy. LDL and acetylated-LDL (AcLDL) were seen bound to the …
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The Role of Protein S-glutathionylation on Ca2+ Signaling in Cultured Aortic Endothelial Cells
In non-excitable cells, oxidative stress impacts both basal and agonist-mediated changes in cytosolic free Ca2+ concentration ([Ca2+]i). An increase in protein S-glutathionylation is a common response to oxidative stress; however, the role of glutathionylation in Ca2+ homeostasis and Ca2+ signaling …
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The structure of cyclic fatty acid monomers isolated from heated linseed oil and their effects on cultured aortic endothelial cells
… of these components in cultured porcine aortic endothelial cells. Using CFAM-oxazoline derivatives, location of unsaturation and ring structures were confirmed using gas chromatography-matrix isolation-Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. The …
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Purification and Characterization of Proteoglycan from Bovine Aortic Endothelial Cells Conditioned Media, and its Interaction with Basic Fibroblast Growth Factor (bFGF)
Cultured bovine aortic endothelial (BAE) cells were found to synthesize and secrete heparan sulfate proteoglycans (HSPG), which bound basic fibrobalst growth factor (bFGF). bFGF is a known mitogen for vascular smooth muscle cells, and is indicated to have a role in some proliferative vascular …
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Vascular endothelial cell-surface proteoglycans
… layers of normal ("cobblestone") bovine vascular endothelial cells in culture. Treatment of the parent molecules with dithiothreitol caused their complete cleavage and permitted the subsequent separation of the larger and smaller subunits on Sepharose CL4B columns. Removal of dithiothreitol by …
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The effect of free fatty acids on endothelial cells
… acids (FFA) are alone sufficient to decrease endothelial cells. This hypothesis was tested by incubating porcine aortic endothelial cells (PAECs) with various oxidized PUFAs and saturated FFAs. Twenty hour exposure of PAECs with the oxidized PUFAs (0-100[mu]M) linoleic acid, eicosapentaenoic …
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Regulation of specific connexins differentially alters gap junction permeability and endothelial cell function
… factor-[Beta][ (TGF-[beta]), prostacyclin, and endothelial nitric oxide synthase (eNOS). siRNA inhibition of connexin 43 in porcine aortic endothelial cells (PAEC) significantly decreased the diffusion distance of Lucifer yellow dye and cytoplasmic calcium levels after mechanical wounding. Wound …
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Lipoprotein receptors in cultured bovine endothelial cells
Endothelial cells take up and degrade both low density lipoproteins and low density lipoproteins which have been modified by acetylation (AcLDL). In this study, receptors that may be involved in the uptake of these lipoproteins were characterized. The cells used were aortic endothelial cells …
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Endothelial cell plasticity as a marker of vascular disease and predictor of adverse outcomes to stress
Endothelial cells (ECs) dynamically sense and adapt to their local biomechanical and biochemical environments, a process crucial for maintaining vascular homeostasis. Loss of this plasticity is implicated in vascular diseases, where endothelial dysfunction and maladaptive responses exacerbate …
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Design of a microreactor for studying the effect of shear stress on angiogenesis in self-assembling peptide matrix
… a self-assembling peptide gel, and bovine aortic endothelial cells. A theoretical model was developed to approximate the permeability of the peptide gel and to quantify the average shear stress on an endothelial cell seeded in a 3D matrix of the peptide gel. Experimental results in a …
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In Vitro Binding and Transport Regulation by Endothelial Cells: Preliminary Studies looking at FIX and IGF-I
Endothelial cells separate the bloodstream from the underlying tissue and play a crucial role in vascular homeostasis. They also form an important barrier for vascular drug delivery. This thesis contains preliminary studies targeted at understanding the mechanisms of binding and transport across …
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The effect of metformin on vascular function and AMP-activated protein kinase activation in Type 2 diabetes
… association between obesity, insulin resistance, endothelial dysfunction and accelerated atherosclerosis, but the molecular mechanisms underlying this link are incompletely understood. Biguanides and thiazolidinediones are widely used in the treatment of Type 2 diabetes to improve glycaemia but …
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Investigating the Role of Phosphodiesterase 1C in Vascular Smooth Muscle Cell Migration and Invasion
… plasticity of human arterial smooth muscle cells (HASMCs) allows them to transition from a “contractile/quiescent” state, in which they regulate arterial tone and blood pressure, to one in which these cells are more “synthetic/activated”. Synthetic/activated HASMCs are migratory and …
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Kinetics study of heat shock protein 70 expression
… endogenous HSP70 expression kinetics. Bovine aortic endothelial cells (BAEC) were heated in a 42oC incubator for up to 5 hours and subsequently held at 37oC for up to 48 hours. Western blot and quantitative protein analysis were performed to measure the extent of HSP70 expression kinetics as a …
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A study on the resistances to oxygen transport in the microcirculation
… work, the oxygen permeability of cultured bovine aortic and human umbilical vein endothelial cell monolayers was measured directly in vitro. The monolayers were cultured on silicone rubber membranes and placed in a carefully calibrated diffusion cell immersed in a 37$\sp\circ$C waterbath. The …
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An Intramural, Tension-modulating Reflex in the Rat Caudal Artery
… either neuronal, vascular smooth muscle, and/or endothelial origins. We have shown that EFS-induced relaxation of intact, phenylephrine (PE) preconstricted rat tail artery (RTA) rings was dependent upon the extracellular concentration of Ca++. Inhibiting either nitric oxide (NO) synthesis with …
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Effect of the Insulin-like Growth Factor (IGF) Axis on the Transport Properties of Endothelial and Epithelial Cells In Vitro
… and IGF-I secreting bovine mammary epithelial cells were tested for cell layer permeability, tight and adherens junction proteins, IGF-IR, and a downstream signaling components of IGF-IR. In comparison with parental cells, IGF-I secreting cells had high levels of IGF-IRs, but low levels of the …
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Chronic Shear Stress Effects on Endothelial Cell Response
… secretion of important regulatory molecules by endothelial cells. Our hypothesis was that inclusion of chronic pulsatile shear stress in our model would lead to changes in endothelial cell release of regulatory molecules. We distinguished between high arterial shear stresses and low venous shear …
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Plasma membrane calcium ATPase 4 (PMCA4) and inflammation of the aortic endothelium
… the circulation to sites of tissue injury. The endothelial expression of cell adhesion molecules, namely the selectin family, mediates the initial adhesive interactions between leukocytes and the endothelium. Immune cell infiltration is often involved in the initiation and progression of several …
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Preparation and Characterization of Electrospun Poly(D, L-Lactide-Co-Glycolide) Scaffolds for Vascular Tissue Engineering and the Advancement of an In Vitro Blood Brain Barrier Model
… PLGA scaffold pressure sodded with C6 glial cells and BAECs (Bovie Aortic Endothelial Cells). The aims of this thesis were to upgrade and refine the existing electrospinner system, develop a BBB scaffold electrospinning protocol, and characterize and evaluate the consistency of the scaffolds …
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