Global ETD Search
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Showing 1 to 20 of 20 for “"antitermination"”.
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NMR Studies on Termination/- Antitermination Complexes of HIV-1 Transcription
… understanding the mechanism of termination and antitermination of HIV-1 transcription. Some of the cysteines in the wild type HIV-1 Tat are required for the Zn2+ dependent interaction with pTEFb, but not needed for the binding of TAR RNA in vitro. These cysteines are readily oxidised and leading …
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INVESTIGATION OF THE NOVEL ANTITERMINATION MECHANISM OF LOAP, A NUSG SPECIALIZED PARALOG
… coupling. NusG is integral to antitermination complexes, where it associates with additional factors to modify the TEC in order to bypass termination events at distinct gene clusters. In Gammaproteobacteria, these complexes enhance the expression of ribosomal RNA operons, …
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Site Directed Mutagensis of Bacteriophage HK639 and Identification of Its Integration Site
… by suppressing transcription, a process known as antitermination. Transcription antitermination in Lambda is mediated by a phage-encoded protein whereas in lambdoid phage HK022, antitermination is mediated by a phage-encoded RNA molecules. Recent results suggest that another bacteriophage called …
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Defining the Requirements for Early Gene Expression in Bacteriophage HK639
… termination to fully express their genes. Antitermination of early gene expression in most lambdoid phages is mediated by an interaction between the N protein and a number of host-encoded factors. Bacteriophage HK022 does not rely on a protein for antitermination. To promote full expression …
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Multiple Posttranscriptional Regulatory Features Control Expression of Ethanolamine Utilization Genes In Enterococcus Faecalis
… which binds RNA to trigger transcriptional antitermination. I determined that induction of expression of several genes in the <em>eut</em> locus is dependent on ethanolamine, AdoCBL and the two-component system. AdoCBL and ethanolamine are both required for induction of <em>eut</em> locus …
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Die Regulation der Transkriptionsantitermination in Escherichia Coli.
… Während der Elongation bewirkt die sogenannte Antitermination das Überlesen von Terminationssequenzen. Dieser zuerst beim Phagen Lambda identifizierte Mechanismus, beruht auf dem Aufbau eines Multi-Protein-Komplexes, bestehend aus den Escherichia coli (E. coli) Nus-Proteinen (A, B, E, G), der …
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Analysis of NusA's roles in DNA repair and damage tolerance pathways in Escherichia coli
… factor that functions in both termination and antitermination. However, its role in DNA repair and damage tolerance has not been explored until recently. Cohen et al. proposed for the first time that NusA functions as a coupling factor in transcription-coupled translesion synthesis (TC-TLS) and …
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Structural insights into RNA binding by NusA and interaction studies of Nun with E. coli Nus factors.
In phage lambda, antitermination is initiated by the lambda-encoded N protein which recruits a number of host proteins called Nus factors. Several of these host proteins which are essential for effective transcription termination and antitermination have been identified, these includes NusA, NusB, …
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Structure determination of bacteriophage [phi]21 N-peptide and boxB RNA complex by NMR spectrocsopy
The antitermination protein N from [delta] and related E. coli bacteriophage interacts with the nut RNA site and host factors to transform RNA polymerase (RNAP) into a termination resistant transcription complex. The [delta], P22 and [phi]21 phage N proteins share a conserved amino-terminal, …
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Functional elements of the promoter, leader and intergenic spacer regions of ribosomal RNA operon(s) of mycobacteria
… of peR. Both leader and spacer regions contain antitermination elements and RNaseIII processing sites. The sequences established for these two regions also showed greater variability than the 168 rRNA gene and are suitable for phylogenetic studies. The sequences of the two rrn operons of …
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Analysis of the Clear Plaque Phenotype of the Bacteriophage HK75
… HK022 and other phages that use RNA mediated antitermination to promote early gene expression. Recent genomic analysis of HK75 and HK022 has confirmed the relatedness of these two phages and place HK75 in the lambdoid family of bacteriophages. Lambdoid phages are temperate and can adopt a …
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Untersuchung der Spezifität von Antiterminationsproteinen in Bacillus subtilis
… Die Expression der Permeasen wird durch Antitermination reguliert. Ein Antiterminationsprotein bindet hierbei an die sogenannte RAT-Struktur der mRNA der jeweiligen Permease und verhindert so die Termination der Transkription. Die Regulation der Aktivität dieser Antiterminationsproteine …
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Structural Studies of Three Factors That Affect the Prokaryotic Transcription Cycle; Microcin J25, LAMBDA Q and T4 GP33
… A structure of Q protein could reveal how Q antitermination occurs and may provide insight into the mechanism of termination. We developed an improved expression and purification protocol for λQ, and performed biophysical characterization on both λQ and the related 82Q. The T4 phage protein …
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Transcriptional Regulation And Autogenous Control Of The Genes For The Beta And Beta' Subunits Of Escherichia Coli Rna Polymerase
… findings suggest the potential involvement of antitermination in the modulation of rpoBa function. Modulation of rpoBa, together with the evidence by others that the global frequency of transcription is influenced by the cellular concentration of RNA polymerase, suggests that rpoBa may play a …
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Regulatory RNAs at the Heart of Sugar Metabolism: New Mechanisms and Novel Discoveries
… degradation event, while EAR uses a processive antitermination mechanism for complete synthesis of the long operon. Furthermore, by using high-throughput sequencing approach we have successfully identified many new regulatory RNA candidates, including various long 5`-UTR, toxin-antitoxin …
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Regulatory intricacies that confer temporal control of conjugation and antibiotic production functions in Streptomyces: new variations on old themes
… the intercistronic region appears to promote antitermination of operon transcription to counteract the effects of the KorB roadblock. Regulation of antibiotic production in Streptomyces typically involves the streptomycete global regulatory mechanism bldA. The sweet potato pathogen …
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Tryptophanase Regulatory Mechanisms in Escherichia coli
… repression and tryptophan-induced transcription antitermination. Recent studies have unveiled two distinct kinetic modes of indole signalling in *E. coli*: a long-lasting but low-level (persistent) signal, and a transient, high-level (pulse) signal. The underlying mechanisms that regulate the …
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Die Regulation der prokaryontischen Transkription auf molekularer Ebene
… werden. Eine weitere Rolle spielt NusA bei der Antitermination. Dieser zuerst im Phagen lambda entdeckte Mechanismus bewirkt, dass die Transkription nicht an einem Terminationssignal abbricht. Das dafür notwendige Antiterminationssignal besteht aus zwei bestimmten RNA-Sequenzabschnitten, boxA …
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Carbon Catabolism in <i>Bacillus subtilis</i>: Global and Molecular Views on the Control of Gene Expression
… Permeasen werden durch transkriptionelle Antitermination kontrolliert. Hierbei kann die mRNA dieser Gene im 5' Bereich eine von zwei alternativen Sekundärstrukturen annehmen. Bei Ausbildung des thermodynamisch stabileren Terminators kommt es zum vorzeitigen Abbruch der Transkription. …
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Regulation der Transkription: Struktur, Dynamik und Wechselwirkungen der carboxyterminalen Domäne von E.coli NusA
… Die lambda N-NusA-Wechselwirkung steigert die Antiterminationseffizienz von NusA wahrscheinlich über eine Stabilisierung der schwachen RNAP-lambda N-Interaktion. Der Vergleich der Struktur von AR1 im ungebundenen Zustand mit der kürzlich gelösten Röntgenkristallstruktur des NusA(350-424)-lambda …