Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 26 for “"antisense RNA"”.
-
Correlating antisense RNA performance with thermodynamic calculations
Antisense RNA (asRNA) strategies are identified as an effective and specific method for gene down-regulation at the post-transcriptional level. In this study, the major purpose is to find a correlation between the expression level and minimum free energy to enable the design of specific asRNA …
-
Knockdown of nfa1 Gene Cloned from Naegleria fowleri by Antisense RNA
… cytotoxicity of N. fowleri to target cells, an antisense RNA or siRNA of nfa1 gene were transfected into N. fowleri trophozoites. By the synthetic dsRNA of nfa1 gene ORF, the expression of nfa1 gene and the Nfa1 protein were knockdowned about 50% and 30%, respectively. However, by antisense RNA …
-
The effect of mutant replication initiation proteins and antisense RNA to the gene on maize streak virus replication
Maize (Zea mays L) is a major crop plant belonging to the family Poaceae, which includes other important cereals such as wheat, rice, barley and oats (Kellogg, 1998). Other grass crops are rye, common millet, finger millet, teff, sugarcane and sorghum. Many of these are important food crops in most …
-
Regulation of DnaA as a transcription factor by modulation of cooperative binding, and by arrA, an antisense RNA
… subtilis. I described the role of a small antisense RNA in the dnaA region, arrA, which represses dnaA. Mutation of the arrA promoter resulted in increased DnaA levels, which were insufficient to disrupt replication initiation but affected cellular physiology and expression of DnaA targets. …
-
The Development of a Thermodynamic Model for Antisense RNA Design and an Electro-transformation Protocol to Introduce Auxotrophic Genes for Enhancing Eicosapentaenoic Acid Yield from Pythium irregulare
… in the EPA biosynthesis in this organism. An antisense RNA (asRNA) thermodynamic model was developed to design new asRNA constructs capable of fine-tuning gene expression knockdown. The asRNA technology is now identified as an effective and specific method for regulating microbial gene …
-
Molecular mechanism of glycogen phosphorylase gene regulation during Dictyostelium development
… obtained through homologous recombination and antisense RNA. Southern analysis revealed that the normal gp1 gene was disrupted in all homologous recombination transformants and in half of the antisense RNA transformants. Finally, for the first time, an extrachromosomal luciferase reporter …
-
Analysis of the role of extracellular signal regulated kinase (ERK5) in the differentiation of muscle cells
… blocked, when ERK5 expression is inhibited by antisense RNA. The effect can be detected also on the expression level of myogenic determination and differentiation markers such as p21, MyoD and myogenin. Another new finding is that stable expression of ERK5 in C2C12 leads to differentiation like …
-
Establishing a positional information assay for brain ventricle mutants and investigating the choroid plexuses in zebrafish
… that a cocktail of krox20, pax2a, shh, and zicl antisense RNA probes hybridizes to domains in the developing brain that reflect anterior, posterior, dorsal, and ventral axis specification. In addition, I have investigated the choroid plexus (CP) cells lining the brain ventricles in the zebrafish. …
-
USING ANTISENSE MESSENGER RNA TO DOWNREGULATE LON MEDIATED PROTEOLYSIS IN ESCHERICHIA COLI
… recombinant proteins. Consequently, a variety of antisense strategies have been examined and shown to effectively control endogenous gene expression and function in E. coli. For this investigation we explored using a 300 base pair sequence of the 5' coding region of E. coli lon gene, including the …
-
Molekularbiologische Untersuchungen zur Eignung Virulenz-relevanter Faktoren als Zielstrukturen für die Entwicklung neuer Antibiotika gegen Staphylococcus aureus
… die bakterielle Zellwandsynthese, den DNA- und RNA-Stoffwechsel oder die Proteinbiosynthese gerichtet. In dieser Arbeit sollten Virulenz-relevante Zielstrukturen für die Entwicklung neuer Antibiotika untersucht werden. Insgesamt wurden sieben Gene analysiert, von denen vier zu Anfang dieser …
-
Epigenetic therapy based on micrornas inhibition for the amelioration of masld phenotype
… due to the complexity of the disorder. MicroRNAs are small non-coding RNAs which regulate gene expression posttranscriptionally, affecting several physiological and pathological processes. Thus, microRNAs have been suggested as potential biomarkers and therapies for different diseases. Among …
-
Development of glucose valves for metabolic engineering applications in E. coli
… through glucokinase expression. Next, inducible antisense RNA and inverting genetic circuits were developed to dynamically control glucokinase expression. With dynamic control, carbon was redirected from endogenous processes only once sufficient cellular resources had accumulated, further …
-
Mechanisms Involved in the Expression of the AmpC Beta-lactamase Gene blaCMY-2 in Escherichia coli
… kb IncI1 plasmid had point mutations in the inc antisense RNA gene that controls IncI1 copy number. 2) Examination of upstream sequence for two parent strains identified a novel divergent tandem blaCMY-2 arrangement flanking an IS5 insertion element. Strains with this feature had 2-fold higher …
-
Proteolytic mechanisms involved in the metastasis of human melanoma cells
… production by these cells (at the level of mRNA as well as activity) as well as expression of plasminogen activator inhibitor PAl-1 and receptors for tPA and uPA (uPAR). UCT-Mel 3 cells expressed uPAR although uPA was not detected. I also examined cells cultured from two metastatic deposits. …
-
Regulation der Metalloproteinasen Stromelysin und Collagenase durch Interferon in retinalen Pigmentepithelzellen
… werden kann. Der Nachweis sollte auf der mRNA-Ebene erfolgen. Als Nachweismethoden dienten die Northern-blot-Analyse, wobei für die Hybridisierung Digoxigenin-markierte antisense-RNA verwendet wurde, die durch in vitro-Transkription gewonnen wurde, sowie die RT-PCR. Als Modell diente die …
-
Identification and Characterization of msaB Gene Involved in Biofilm Formation and Virulence in <i>Staphylococcus aureus</i>
… SAUSA300_1294 (<em>msaC</em>), and antisense RNA, <em>msaR</em>. The mechanism of regulation of <em>msaABCR</em> operon and the function of individual genes were not clearly known yet. This study defines the role of <em>msaB</em>, the second gene of the <em>msaABCR</em> operon, which …
-
Biochemical and genetic properties of HPRT Cape Town
… function of the X chromosome carrying the maternal and presumably normal HPRT allele. The daughter's enzyme prepared from erythrocyte lysates exhibited intermediate enzyme activity between that of the proband and a normal control. This result suggests that the daughter is an obligate …
-
Functional gene analysis in cultured vertebrate cells using siRNA mediated gene silencing
… kann sequenzspezifisch durch small interfering RNAs (engl. kleine interferierende RNAs, siRNAs) in einem als RNA Interferenz (RNAi) bezeichneten Prozeß herunterreguliert werden. Die Anwendung von RNAi Technologie erlaubt die systematische Analyse von Genexpression und Genfunktionen. In dieser …
-
Identification and characterisation of KZFPs directly regulated by p53
… potential by acting as enhancers or alternative promoters, influencing nearby genes. The KZFP family presents an interesting conundrum; the family is highly evolutionary dynamic, with new members appearing at every phylogenetic branch since their inception in tetrapods, yet their binding …
-
Discovery and Validation of New Regulatory RNA Elements in Chlamydia trachomatis
… of a newly defined family of non-coding RNAs (ncRNAs) that are differentially expressed during the developmental cycle and the induction of persistence and reactivation (Using IFNγ and Carbenicillin). Non-coding RNAs were initially identified using an intergenic tiling microarray and were …
Page 1 of 2