Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"antigen specificity"”.
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Statistical physics of T cell receptor development and antigen specificity
… We propose that this is the mechanism for the specificity of T cells for unknown pathogens. The T cell development model is also used to discuss one way in which host genetics can influence the selection of T cells and concomitantly the control of HIV infection. A model of the T cell selection …
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Decoding Human T Cell Immunity With Single-Cell Multi-Omics
… single-cell studies, and computational tools for antigen specificity prediction. Throughout the next chapters, I introduce Cell2TCR, an open-source framework for cross-donor TCR sequence comparison, and make the case for convergent clonal selection after infection through the discovery and …
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Characterization of viral- and fungal-reactive CD4+ T cells in healthy and diseased
… while maintaining tolerance to harmless antigens. Their antigen specificity, diversity and functional properties are poorly characterized. This thesis is a cumulative work of three manuscripts that cover the investigation of antigen-specific CD4+ T cell responses in the contexts of …
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Dysregulation of of phospholipid-specific phagocytosis by B1 B cells in diet-induced obese mice
… in the biological membrane and a classical antigen recognized by B1 B cell-derived natural antibodies. These antibodies play important roles in immune defense as well as tissue homeostasis. Here we report that B1 cells preferentially phagocytose PtC-coated beads, differing from that of …
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Recovery of T cell receptor variable sequences from 3' barcoded single-cell RNA sequencing libraries
… features, including variable sequences of T cell antigen receptors (TCRs) that confer antigen specificity. Incorporation of TCR sequencing into scRNA-seq data could identify cells with shared antigen-recognition, further elucidating dynamics of antigen-specific immune responses in T cells.
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CD8+ lymphocyte and progenitor cell mobilization during acute psychological stress and betaadrenergic stimulation
… and NKT-like cells. Chapter 4 examined whether antigen-specificity could modulate CD8TL stress- and \(\beta\)AR-sensitivity. CMV-specific cells demonstrated enhanced mobilization compared to the total-memory CD8TL and the total Epstein-Barr virus (EBV) population. In Chapter 5, we demonstrated …
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Molecular mechanisms of T cell homing in Hodgkin's lymphoma : implications for T-cell-based therapies
… ccr5), which was typically independent of target antigen specificity, CD4/CD8 and donor status. However, lack of CCR7 expression and an inability to capture to VCAM-1 in a chemokine dependent manner suggested that clones expanded in vitro using existing protocols may be inefficient at trafficking …
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Design of Novel Antigen-Specific Immunotherapies for the Treatment of Autoimmune Disorders
… systems – the immune system. A dysregulation of antigen recognition is at the center of autoimmune disorders, and can occur in a variety of host tissues throughout the body. Further complicating these diseases is the high degree of variability in terms of clinical manifestation. Traditional …
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The Role of ZMYND8 in Immunoglobulin Class Switch Recombination
… component of the antibody response but preserves antigen specificity. CSR is initiated by the enzyme activation induced cytidine deaminase (AID), which produces nucleotide mismatches in actively transcribed immunoglobulin heavy chain (Igh) switch donor and acceptor DNA. The 3' Regulatory Region …
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Clonal Studies of Human B Cells
… determined by their origin, developmental stage, antigen specificity, and function.</p><p>Evidence suggests that human innate-like B cells (i.e., marginal zone and/or B1-like B cells) develop during fetal life. However, the characteristics of human fetal B-lineage cells are less understood. Recent …
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Investigating the phenotype and function of tissue-resident B cells in mouse and human non-lymphoid organs
… these B cells into NLOs was independent of their antigen specificity. Using strains of genetically modified mice with higher (PI3Kδ E1020K-B, Siglec-G-/-) or lower (μMT-) numbers of tissue-resident B cells in NLOs, we tested the function of these cells in the context of urinary tract infection. …
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Constraints on the CD8 T cell response in chronic HBV infection
… T cells from CHB patients, regardless of their antigen specificity, were impaired in their ability to produce IL-2 and proliferate upon TCR dependent stimulation. By contrast, these CD8 T cells had preserved production of the proinflammatory cytokines IFN-γ and TNF-α. We provide evidence that …
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Rational engineering strategies to improve antibody fragment pharmacokinetics and bioavailability
… classes of biotherapeutics due to their unique antigen specificity and their ability to be engineered against diverse disease targets. There is significant interest in utilizing truncated antibody fragments, including single-domain antibodies (sdAb), as therapeutics, as their small size affords …
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Development of a Neoepitope Prioritization Platform to Identify Immunogenic Neoantigens from Oncogenic FGFR3-TACC3 Fusion in Glioblastoma
… the FGFR3-TACC3 fusion as a tumor-specific neoantigen source and establishes an end-to-end computational and experimental framework for the identification, prioritization, and validation of immunogenic neoantigens derived from chimeric RNAs. The FGFR3-TACC3 fusion, generated through an …
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Improving methods for cytokine immunotherapy of cancer
… More surprisingly, immunocytokine antigen specificity and Fc[gamma]R interactions did not appear necessary for therapeutic efficacy or biodistribution patterns, as immunocytokines with irrelevant specificity and/or inactive mutant Fc domains behaved similarly to tumor-specific …
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FUNCTIONAL CHARACTERIZATION OF THE INTERACTION BETWEENP. AERUGINOSA AND DENDRITIC CELLS TRIGGERING THE DIFFERENTIATION OF PATHOGENIC TH1/17 CELLS IN CYSTIC FIBROSIS.
… infiammatoria mediata da Pa, e un saggio di antigen-specificity per indagare se Pa inducesse direttamente il trans-differenziamento dei linfociti protettivi cTh17 in patogenici co-produttori di IFNγ/IL-17. I nostri dati dimostrano che i subsets patogenici (Th1/17+ e pTh17) sono …
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The Role of TCR Specificity in the Regulation of the Immune Response to Self and Commensal Antigens
ABSTRACT OF THE DISSERTATION The Role of TCR Specificity in the Regulation of the Immune Response to Self and Commensal Antigens by Stephanie K. Lathrop Doctor of Philosophy in Biology and Biomedical Sciences: Immunology) Washington University in St. Louis, 2010 Dr. Chyi-Song Hsieh, Chairperson The …
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Multimodality Imaging of Tumor Vasculature and Metabolism
… and blocking studies were conducted to validate antigen specificity. It is recognized that cancer cells exhibit highly elevated glucose metabolism compared to non-tumor cells. In chapter 4, I have applied in vivo optical imaging to study dynamic uptake of a near-infrared dye-labeled glucose …
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Autoregulatory CD8 T-Cells Modulate CNS Autoimmune Disease by Targeting Encephalitogenic CD4 T-Cells
… T-cell population in the MS CNS lesion, their antigen specificity and function remains to be elucidated. Using a murine model of MS, experimental autoimmune encephalomyelitis (EAE), we are currently evaluating the role of these poorly studied myelin antigen-specific CD8 T-cells in the context …
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