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Showing 1 to 20 of 27 for “"anticodon"”.
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Substitution of the Anticodon Loop of Yeast Tyrosine Transfer Ribonucleic Acid (Rna)
Procedures for replacing residues 33-35 in the anticodon loop of yeast tRNA('Tyr) with any desired oligonucleotide have been developed. Half-molecules of tRNA('Tyr) from partial digestions with ribonuclease A or T(,1) were paired to create a gap in the anticodon loop. An oligonucleotide was …
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Arginyl-tRNA Modifications Modulate Anticodon Domain Structure, Function and Dynamics in Escherichia coli.
North Carolina State University Theses Biochemistry.
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Characterization of the Operational Trna Code for Amino Acids Through Supervised Machine Learning
… of tRNAs outside and independent of the anticodon. Previous efforts to uncover these rules have included experimental studies using synthetic and chemically modified transfer RNAs (tRNAs), as well as computational analyses aimed at predicting the cognate amino acid from tRNA primary …
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Characterisation of the Essential t<sup>6</sup>A tRNA Modification Enzymes and Evaluation as a Potential Novel Antimicrobial Target
… and in the correct matching of the codon-anticodon pair at the ribosome. Two positions on the tRNA molecule are significant in this last action; positions 34 and 37. Position 34 is the first base in the anticodon, and pairs with the last base in the codon during translation; modifications …
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The Cellular and Molecular Dynamics of the Queuosine Modification in Transfer RNA: Definition, Modulation, Deficiencies and Effect of the Queuosine Modification System
… nucleosides incorporated into tRNA<sup>asp</sup> anticodon stem/loop structures, and in triad complexes of tRNA<sup>asp</sup> with mRNA and tRNA<sup>phe</sup>. The results of this research identify indistinguishable energetic parameters for complexes of queuosine-modified anticodon loops when …
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BIOCHEMICAL ANALYSIS OF TWO DISTINCT METHIONYL-TRNA SYNTHETASES
… to use long-range communication between its anticodon binding and catalytic active sites. MetRS also exhibits structural variability in different organisms including different oligomeric states and appended domains. In this dissertation, our goal was to understand how long-range communication …
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NMR studies of RNA binding domains of human lysyl aminoacyl tRNA synthetase
… packaging into new HIV-1 particles. Since the anticodon binding (ACB) domain of hLysRS is required for proper recognition of its cognate tRNA, NMR studies of the ACB domain were conducted to enhance our understanding of how hLysRS interacts with these RNAs during protein biosynthesis as well as …
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The Development and Application of Methods to Study the Evolution of Specificity, Allostery, and RNA -Protein Interactions in Translation
… consists only of the catalytic domain while the anticodon binding domain and the editing domain were later additions that evolved as specificity emerged in the AARSs. In the second step of translation, the elongation factor Tu (EF-Tu) binds to all the standard aminoacyl-transfer RNAs and …
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The Role of Queuine Deficient tRNA in Translational Control
… hypomodified tRNA. The loss of queuine in the anticodon may change the interaction of the tRNA's anticodon with its codon. Therefore, the translational complex may stall looking for a rare tRNA, or the stability of the complex adding an amino acid to a peptide chain may be changed by the loss …
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The Ribosome Discriminates the Structure of the Amino Acid at its Peptidyl-Transferase Center
… miscode due to mutations that lie outside the anticodon as well as data from the unnatural amino acid mutagenesis field that shows that different unnatural amino acids, when esterified to the same suppressor tRNA, produce different amounts of protein, suggest that aa-tRNA selection is …
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Biochemical and Structural Studies of TGT and MiaA: Key Enzymes Involved in Two Types of Hypermodifications
… of A37 (position 3' adjacent to the anticodon region) starts with the formation of (isopentenyl)-adenosine (i6A) and in E. coli, the first step is catalyzed by the enzyme MiaA. Through BLAST search, a few protein sequences highly homologous to MiaA were identified. These …
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Untersuchungen zu Struktur-Funktionsbeziehungen in der tRNA-ähnlichen Struktur des Rüben-Gelbmosaik-Virus
… waren. Die TLS-Varianten waren außerdem in Anticodon-Schleife und L1-Schleife verschieden und die Transkriptionseffizienzen wurden in nicht valyliertem und valyliertem Zustand ermittelt. Mit diesen in vitro Experimenten sollte untersucht werden, ob die Länge und Sequenz der beschriebenen …
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Determinants of translational efficiency in Saccharomyces cerevisiae
… in which I investigate the role of a conserved anticodon tRNA modification in determining the rate of translation elongation and the phenotypic consequences of its loss for budding yeast. In Chapter 3, I discuss the regulation of translation initiation to introduce Chapter 4, in which I explore …
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The physiological consequences of loss of tRNA thiolation in Saccharomyces cerevisiae
… the more complex mcm5 s2 U34 modification on the anticodon wobble uridines of tGliUUC, of tGgluUUG and tLysUUU tRNAs in a variety of organisms. It has become increasingly clear that tRNA modifications serve to alter the properties of tRNA molecules, and that tRNA modifications can impact the …
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Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase with group I intron RNAs
… cognates of the aminoacyl-acceptor stem and D-anticodon arms, respectively. Further, three-dimensional graphic modeling, supported by biochemical data, shows that conserved regions of group I introns can be superimposed over interacting regions of the tRNA in a Thermus thermophilus …
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Selenoprotein-Biosynthese in Archaea
… tRNASec von M. maripaludis, bei der eine Base im Anticodon-"Loop"ausgetauscht worden war, konnte im heterologen Wirt E. coli Selenocystein inserieren. b) Die Natur und die Lokalisation des archaeellen SECIS-Elements konnte durch die heterologe Expression eines Selenoprotein-Gens von M. jannaschii …
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Probing substrate specificity of RNA repair protein Pnkp/Hen1 from Anabaena variabilis
… as well as the original tRNA are cleaved at the anticodon loops in vitro with Colicin. Then protein Pnkp/Hen1 is used to repair each pair of the cleaved RNAs, and it is found that truncated versions of RNAs could be repaired as efficiently as the original tRNA, suggesting that Pnkp/Hen1 is not …
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Single Molecule Förster Resonance Energy Transfer and Super Resolution Force Spectroscopy Study of Elongation Factor G Evolution, Conformation, and Function
… in translocation as they interact with codon-anticodon minihelix formed between mRNA and the A-site tRNA. We investigated EF-G mediated power stroke and translocation fidelity via mutagenesis of these two residues. Five substitution mutations were expressed (H584K, H584E, H584Q, Q508K, and …
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Structure Function Analysis of Adenosine Deaminase Acting on tRNA (ADAT2) from Trypanosoma Brucei
<p>tRNA is edited at the wobble position of the anticodon to accommodate the degeneracy of the genetic code. The enzymes responsible for deamination of adenosine 34 to inosine are found in all organisms and in all but a few cases are essential for life. In bacteria the reaction is carried out by …
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GUIDE RNA-DEPENDENT AND INDEPENDENT tRNA MODIFICATIONS IN ARCHAEA
… modified than others. Two such regions are the anticodon loop, and the TψC stem. In the halophilic euryarchaeon <italic>Haloferax volcanii</italic>, tRNA<super>Trp</super> and tRNA<super>Met</super>, both of which are transcribed as intron-containing pre-tRNA forms, contain Cm34 and ψ54, …
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