Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 85 for “"antibody-mediated"”.
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Towards an antibody mediated resistance against Geminiviruses
In this study, single chains (scFvs) against key proteins of the Tomato leaf curl virus-India were generated using phage display and/or hybridoma technologies. Purified AV1 (coat protein) and AC1 (replicase enhancer) viral proteins were used for mice immunization and screening of phage display …
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Antibody-Mediated Modulation of Polyclonal Immune Responses
… immune response. Infusion of a monoclonal antibody prior to immunization with its cognate protein antigen resulted in two key observations. Firstly, antibody can increase the overall magnitude of the germinal center (GC) and plasmablast (PB) response. Secondly, this enhancement is …
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Optimisation of the Investigation of Antibody-Mediated Dysglycaemia
… diabetes and labile glycaemic control. Antibody depletion can effectively treat either condition in many cases. This research aimed to target major limitations of existing diagnostics, specifically, that anti-insulin antibody (IA) testing alone does not establish whether antibodies alter …
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The molecular basis for evasion of antibody-mediated neutralization in flaviviruses
… neutralizing antibodies; a phenomenon known as antibody-mediated neutralization resistance. Most of the critical mutations associated with antibody resistance are located in the envelope protein domain 3 (ED3). In West Nile virus (WNV), and in other flaviviruses, mutations in ED3 are found …
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Development of recombinant antibody mediated resistance against Tomato yellow leaf curl virus
In this study, we exploited the expression of specific scFv fragment in plant cells for suppression of disease symptoms caused by TYLCV infection. The C1, V1 and V2 genes encoding Rep, CP and MP, respectively, were cloned into the TOPO vector and subsequently into pGEX-5x3 and pMALc2x expression …
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Investigating the Effect of Antibody-Mediated Feedback on Ongoing Germinal Center Responses
… in the form of long-lasting, high-affinity serum antibody titers, which requires the participation of B cells in the germinal center (GC) response. It is well-established that pre-existing antibodies can influence the outcome of future humoral responses; however, whether antibody from an ongoing …
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Investigation of novel therapeutic strategies in B cell and antibody mediated disease
… differentiated B cells are responsible for antibody generation, a key component of adaptive immunity. IgG antibodies play an important role in defence against infection but can be pathogenic in some autoimmune diseases and in solid organ transplantation. In addition to antibody generation, …
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Effects of variation in N-linked glycosylation site on antibody mediated neutralization in PRRSV
… have mutants that can escape the neutralization antibody response. Various studies have been done to indicate that the envelope glycoprotein 5 bears a major neutralizing epitope. The ectodomain of glycoprotein 5 in PRRSV has a neutralization epitope which is flanked by three N-linked …
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Anti-Nucleic Acid Antibody-Mediated Cross Presentation of An Antigen and Its CD8+ T Cell Activation
… fragment (scFv) of a nucleic acid-hydrolyzing antibody, 3D8. As the CD8-T cell epitopes, chicken ovalbumin amino acid 257-264 (OVA257-264-SIINFEKL) and 250-264 (OVA250-264-SGLEQLESIINFEKL) were used for 3D8 scFv fusion partners. Purified 3D8 scFv-OVA fusion proteins with ~95% purity retained …
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Human antibody-mediated immunity against infection and parasite fecundity in Schistosoma mansoni and Schistosoma haematobium infection
… of this thesis was to advance understanding of antibody-driven immune responses that partially protect against infection and reduce fecundity in human schistosome infections. The relative contribution of exposure to infection and IgE antibody responses against schistosomes were explored using a …
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Neuropsychiatric symptoms in thymoma-associated and non-thymoma myasthenia gravis
… gravis (MG) is an acetylcholine receptor antibody- mediated disease targeting the neuromuscular junction resulting in fatigable muscle weakness. A number of reports have suggested a high prevalence of psychiatric symptoms amongst MG patients. Approximately 10% of MG subjects are found to …
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High throughput measurement of antibody and complement mediated immunity to meningococcal disease
… bactericidal titres of ≥1:4. The importance of antibody and complement mediated immune mechanisms is also emphasised by the increased susceptibility of complement deficient individuals to meningococcal disease. <br></br><br></br> However, the absence of bactericidal activity does not necessarily …
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Toxic effects of dietary methylmercury on immune system structure, function, and development in the American kestrel (Falco sparverius)
… kestrels. Immunological endpoints included cell-mediated immunity (CMI) using the phytohemagglutinin (PHA) skin-swelling assay and antibody-mediated immune function using the sheep red blood cell (SRBC) hemagglutination assay. Hematology profiles were evaluated over time in adult males and …
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Study of Physiologic and Immunologic Incompatibilities of Pig to Human Transplantation
… Second, immunologic incompatibilities due to antibody recognition of antigens resulting in antibody-mediated rejection were studied. Identification of relevant targets was systematically approached through evaluation of a known xenoantigenic protein fibronectin from genetically modified pigs. …
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Autoantibodies From Patients With Nonallergic Asthma Cause Cytotoxic Injury To Airway Epithelial Cells.
… of nonallergic asthma, we examined autoantibody-mediated cytotoxicity to airway epithelial cells using purified IgG antibodies from patients with nonallergic asthma. METHODS: IgG antibodies were purified from patients with nonallergic asthma using by protein A column. IgG …
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A comparative analysis of cowpox virus (CPV WT) and a deletion mutant lacking the gene encoding the inflammation modulatory protein (CPV IMP)
… proteins C3 and C4, thereby preventing complementmediated opsonisation of virus, antibody-mediated lysis of infected cells and migration of inflammatory cells into the site of infection. VCP also possesses heparin binding sites.
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The Impact of IL-9 on Disease in Murine Models of Systemic Lupus Erythematosus
… erythematosus (SLE) is a relapsing-remitting antibody-mediated autoimmune disease characterized by systemic immune complex deposition. A subset of SLE patients has been shown to have elevated CD4+IL-9+ T cells as well as increased secreted IL-9 and Il9 mRNA compared to healthy controls. In …
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