Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 56 for “"antibody fragments"”.
-
Pretargeted cancer radioimmunotherapy and radioimaging using antibodies and antibody fragments
… Using phage display technology, TF binding scFv fragments were selected from the McCafferty antibody library. The selected scFv clones were characterized in vitro for their TF specificity and cell binding properties by ELISA and flow-cytometry assay. The selected TF specific clone (9C-scFv) was …
-
Genetic engineering of antibody fragments for the detection of illicit drugs
… focused on the development of a repertoire of antibody fragments against various illicit drugs including morphine-3-glucuronide (M3G), amphetamine and tetrahydrocannabinol (THC). Antibody libraries were generated from murine, avian and leporine immune systems. A number of antibody fragments …
-
Production and characterisation of novel recombinant antibody fragments against sialic acid
… antisialic acid antibodies.A single-chain antibody fragment (scFv) library was constructed from a chicken that was immunised with a novel synthetic sialic acid protein-conjugate (Neu5Gchumanserum albumin). The scFv library was biopanned using a second novel …
-
Generation and application of recombinant antibody fragments for prostate cancer detection.
… and selection of murine and avian recombinant antibody fragments to free PSA (fPSA) was performed. Animals were immunised, RNA extracted and cDNA synthesised. The antibody variable genes were amplified by PCR, gel-purified and cloned into a phagemid vector. Electrocompetent cells were …
-
Biophysical characterization of high affinity engineered single chain Fv antibody fragments
High affinity antibody binding interactions are important for both pharmaceutical and biotechnological uses. However, designing higher affinity interactions has remained difficult. Both high affinity interactions from nature and the results from directed evolution affinity maturation processes may …
-
Structural study of human antibody fragments with specificity for Mucin-1 antigen
… the stability, solubility and the size of the antibody determines their effectiveness in cancer therapy. Smaller antibody fragments are advantageous over full-size antibodies, since small antigen binding molecules can efficiently penetrate solid tumors. As a first step towards the design of a …
-
In vitro selection and characterisation of human anti-HIV-1 antibody fragments
… libraries of HIV-1 patients. Two single-chain antibody libraries (M325 and K530) were constructed from two HIV-1 long-term non-progressors, whose sera showed cross-neutralising activities against various HIV-1 strains across a range of clades. In each library, total RNA was extracted from blood …
-
Effect of univalent antibody fragments on the fertilizability of Rana pipiens eggs
Thesis (M.S.)--Michigan State University. Dept. of Zoology, 1971
-
Characterisation of llama antibody fragments able to act as HIV-1 entry inhibitors
… masking. As a result, the neutralising antibody response in HIV-1 infection and post-immunisation is generally narrow, and only a handful of broadly neutralising monoclonal antibodies have been reported. In this thesis, the isolation and characterisation of novel, broadly neutralising …
-
Genetic engineering of recombinant anti-mycolic acid antibody fragments for use in tuberculosis diagnostics
… library was previously used to obtain monoclonal antibody binders to mycolic acids. In total 11 binders were obtained of which one was selected (MAC10) for further investigation by genetic engineering as presented in this dissertation. The antibodies of the Nkuku phage display library are in the …
-
Development of high throughput screening systems for the efficient production of antibody fragments in Escherichia coli
Recombinant antibodies and antibody fragments have become powerful tools for therapy, in vivo and in vitro diagnostics, and laboratory research. However, the production of antibody fragments in high yield for preclinical and clinical trials can be a serious bottleneck in drug discovery. This …
-
The role of purified antibodies and antibody fragments in the enhancement and the acute destruction of mouse allografts
Contains fulltext : mmubn000001_02657666x.pdf (Publisher’s version ) (Open Access)
-
Optimized Microbial Recombinant Production of HIV-1 Anti-Envelope Antibody Fragments with Applications to Single Particle Tracking of Virus Assembly
<p>In my findings, I have established a set series of protocols to recombinantly produce, purify and apply various fluorescent probes in vitro for the fluorescent labeling and study of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein during HIV viral assembly. There remains …
-
Generation and characterization of single chain antibody fragments binding to the coat protein of grapevine leafroll associated virus 2 and 3
… on the vine produced. The feasibility of antibody-based resistance has been demonstrated for plant viruses. It has been shown that plants that express antibodies binding to viral coat proteins gain a degree of protection against the virus in question. The rationale of this work was to …
-
Engineering and characterization of single chain antibody fragments (scFvs) specific to key enzymes in polyamine biosynthesis and manipulation of polyamine pathway by constitutive expression of recombinant ODC and SDE enzymes in transgenic tobacco
… the pathway by combining genetic engineering and antibody technology approaches. ODC a key enzyme in the polyamine biosynthesis, was amplified from a human prostate cDNA library and subcloned into plant expression vector for transient expression in the apoplast, cytosol and endoplasmatic …
-
Antibody detection of Burkholderia pseudommallei and Burkholderia mallei
… found to be visually very similar. A monoclonal antibody raised to B. mallei LPS has been found to recognise both B. mallei and B. pseudomallei strains. However, in a small number of B. pseudomallei strains a visually atypical LPS profile has been demonstrated. A monoclonal ant ibody rai sed …
-
Engineering antibodies for improved targeting of solid tumors
… the transport phenomena that contribute to poor antibody distribution in tumors, and engineer novel antibody variants with improved targeting properties. Previous studies identified a core set of parameters that impact tumor uptake including antibody size, binding affinity, plasma clearance rate, …
Page 1 of 3