Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 2399 for “"antibody"”.
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Development of monoclonal antibody cell culture processes and antibody characterisation
… an end-to-end laboratory-scale monoclonal antibody production process, including upstream cell culture, downstream purification and analytical characterisation. A CHO-DP12 cell line engineered for anti-IL-8 mAb expression and a cNIST mAb-producing CHO cell line were employed to investigate …
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Engineering intracellular antibody libraries
The goal of this research is to understand how three different parameters affect single chain variable fragment (scFv) binding capacity. The parameters that were varied include the number of variable complementarity determining regions (CDRs), the number amino acids used to diversify CDRs, and …
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Determinants of antibody specificity
… Development liabilities, including antibody aggregation and nonspecificity, can lead to problems during production and poor pharmacokinetics (PK). The exact structural and sequence determinants causing this poor developability are unknown, which leads to inexact methods to correct …
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Genentech: Purification of Monoclonal Antibody
Lucentis, a monoclonal antibody fragment produced in Escherichia coli, is used to treat wet age-related macular degeneration, one of the leading causes of legal blindness. The objective of this thesis is to analyze the process of antibody fragment separation and to purify a solution of "Lucentis …
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Antibody activity of rabbit macroglobulin
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Common variants in antibody deficiencies
… genetic architecture of CVID, SIgAD, and serum antibody production production, and I adduce my findings in an argument for the relevance of common-variant analysis in the study of IEIs.
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Antibody engineering for cancer therapy
… cancer. In this Thesis, novel antibodies and antibody-conjugate against two tumor antigens, AF-20 antigen and human aspartyl (asparaginyl) [beta]- hydroxylase (HAAH), were developed. Previously, these two tumor antigens have been shown to be present on a variety of tumor cells, while they have …
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Antibody engineering for tumor immunotherapy
… therapy may be improved is the retention time of antibody in the tumor relative to normal tissue. In this Thesis, we have attempted to elucidate the mechanisms that are most influential to improving antibodies as cancer therapeutics. Carcinoembryonic antigen (CEA) has long been identified as a …
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Generation of chickenized 3D8 scFv antibody from mouse monoclonal anti-DNA antibody by CDRs-grafting
… mouse is a catalyric anti nucleic acid antibody that has ability of cellular internalization and DNA/RNA-hydrolyzing activity. It has been reported that cells treated with 3D8 scFv protein showed the down-regulation against replication of RNA-genomed viruses and this anti-viral activity …
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Nanocomposite immunosensor for anti-transglutaminase antibody
… Thus, the concentration of anti-transglutaminase antibody (anti-tTG) in human sera is an important analytical marker for the diagnosis of CD. An immunosensor is a type of biosensor that has an antigen or antibody fragment as its biological recognition component. The specificity of the molecular …
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Intracellular delivery of rabbit monoclonal antibody
… showed that approximately 60% of delivered antibody bound to the antigen proteins. The delivered RabMAb against NF-KB apparently blocked the nuclear translocation of NF-KB introduced by TNF-a. The success of delivering the three rabbit monoclonal antibodies with binding or inhibiting …
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Recall Germinal Center and Antibody Responses
… to viruses or their antigens elicits anamnestic antibody responses that produce antibodies that are of greater magnitude and affinity compared to those induced after primary exposure to antigen.The anamnestic nature of the response is a result of the recall of memory B cells (MBCs) that have …
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Antibody-Effector Functions in Antiviral Response
… arm of the immune system. Interactions of the antibody's fragment crystallizable (Fc) domain with Fc receptors (FcR) on effector leukocytes is essential for proper immunological function and bridges the humoral with the cellular components of immunity. While the Fc domain of immunoglobulin G …
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Antibody Dependent Enhancement of Visceral Leishmaniasis
… disease severity hint at the possibility of antibody dependent enhancement of infection (ADE). Herein we explore the possibility of extrinsic ADE as a result of heterologous exposure to <em>L. major</em> and <em>L. infantum in vitro</em>. ELISA was done to verify cross reactivity of <em>L. …
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TRIM21 Mediated Antibody-Dependent Intracellular Immunity
… such as opsonisation, complement activation, antibody-dependent cytotoxicity and receptor blocking. The discovery of TRIM21 as a cytosolic antibody receptor has revolutionised our understanding of humoral immunity and revealed that antibodies can mediate protective immunity inside cells. …
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Developing Antibody Tools against Glycine Receptors
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Cost modeling for monoclonal antibody manufacturing
The Novartis BioPharmOps division is responsible for manufacturing large molecule products, including monoclonal antibodies, for late stage clinical trials and commercial sales. The BioPharmOps site in Huningue, France is expanding their product line but is also trying to reduce costs; cost …
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