Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"anti-GFP nanobody"”.
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Engineering protein components for living electronics
… an allosteric ferredoxin through insertion of an anti-GFP nanobody. I demonstrate that a ferredoxin which contains a specific anti-GFP nanobody insert requires co-expression with GFP to display electron transfer activity. These studies lay the groundwork for further development of biological …
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Investigating how mitophagy is regulated by the metabolic context
… surface using a conditionally stable anti-GFP nanobody. Then, I implemented this mitochondrial APEX2 proteomics approach to screen for new proteins regulating hypoxia-induced mitophagy by a glycolytic or OXPHOS-promoting metabolic context in ARPE-19 cells. Here, I present the ongoing …
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Synthesis and characterisation of peptide-based probes for quantitative multicolour STORM imaging
… characterisation of peptide-based probes for quantitative multicolour STORM imaging by Edward Taylor Current single molecule localisation microscopy methods allow for multicolour imaging of macromolecules in cells, and for a degree quantification on molecule numbers in one colour. However, that …
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RNA Dysregulation in a Novel Human Model of TDP43 Proteinopathy
… mutation or overexpression. We engineered TDP43-GFP induced pluripotent stem cells, where GFP was knocked into the C-terminus of endogenous TARDBP. In MNs, expression of an anti-GFP nanobody fused to a strong nuclear export signal (NES) allows for rapid mislocalisation of TDP43 into the …
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Applications of yeast display in synthetic biology with a focus on biological synthesis and recycling of plastics
… where instead of immobilising an antibody on agarose beads, the antibody is displayed on the yeast surface, effectively using yeast as beads. As a proof of concept, an anti-GFP nanobody was used to capture GFP from bacterial lysates and GFP-tagged LNX1 protein from mammalian cell …