Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 126 for “"anti-G"11 antibody"”.
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Genetic engineering of recombinant anti-mycolic acid antibody fragments for use in tuberculosis diagnostics
… library was previously used to obtain monoclonal antibody binders to mycolic acids. In total 11 binders were obtained of which one was selected (MAC10) for further investigation by genetic engineering as presented in this dissertation. The antibodies of the Nkuku phage display library are in the …
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Analysis of two methods of isometric muscle contractions during the anti-G straining maneuver
… of isometric muscle contractions during the Anti-G Straining Maneuver (AGSM). 12 subjects (ages 18 to 38 yrs, height 176.8 +/- 7.4 cm, body mass 78.8 +/- 15.6 kg, percent body fat 14.3 +/- 6.6%) participated in the study. The study was a one-way within-subject design with test conditions …
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Construction and characterisation of anti-tumour antibody-based proteins for use in diagnostics and therapeutics
Considerable success in the use of monoclonal antibodies (mAbs) as a targeted approach to cancer therapy has stimulated rapid development in this field of research. Due to the complex nature of the tumour microenvironment many novel antibody formats have been designed to expand on the therapeutic …
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Role of envelope compactness and glycosylation in HIV-1 resistance to neutralising antibody responses
… the mechanisms used by HIV-1 to evade antibody neutralisation may contribute to the design of a high-coverage vaccine. This thesis explores the mechanism used by a Tier 3 virus leading to its high antibody neutralisation resistance phenotype. This thesis also describes how the glycans …
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Antibody-Based Detection of 3-Methylglutaconic Acid
… treatment efficacy, the development of a simple antibody-based assay was explored. Using immune serum from the inoculation of rabbits with a 3MGA-conjugated keyhole limpet hemocyanin (KLH) immunogen, several enzyme-linked immunosorbent assay (ELISA) formats were investigated to find the most …
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Identification of Anti-Beta2 Glycoprotein I Auto-antibody Regulated Gene Targets in the Primary Antiphospholipid Syndrome Using Gene Microarray Analysis
Anti-Beta2-Glycoprotein I antibodies (anti-2GPI) are strongly associated with thrombosis in patients with primary antiphospholipid syndrome (PAPS). Anti-2GPI activate endothelial cells (EC) resulting in a pro-thrombotic and pro-inflammatory phenotype. In order to characterise EC gene regulation …
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Immunohistochemical characterization of neuronal cilia in the rat central nervous system.
An anti-G"11 antibody was used to label neuronal cilia throughout the rat central nervous system. Immunoreactive cilia were observed in every examined region of the rat CNS, but not in monkey or mouse tissue. Antibodies to G"q and G"q/11 failed to label cilia. Immunoreactive cilia were observed as …
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Anti-GD1a antibody targeted disruption of the node of Ranvier in a mouse model of acute motor axonal neuropathy
… paralysis. The axonal variant is associated with anti-GD1a ganglioside antibody-dependent, complement-mediated injury to the peripheral axon with conduction block. The blood-nerve barrier (BNB) relatively protects axons from factors in the extra-neural environment; however, it does not extend over …
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Evaluating the neutralizing antibody response to HIV-1 membrane proximal external regional; Implications for vaccine design
Inducing broadly neutralizing antibodies targeting the HIV-1 envelope is thought to be crucial for developing an effective vaccine. The Membrane Proximal External Region (MPER) within the HIV- 1 gp41 envelope is a promising vaccine target. The MPER is highly conserved, functionally constrained, …
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Generation and application of recombinant antibody fragments for prostate cancer detection.
… the diagnosis of PCa. However, prostate-specific antigen (PSA) and its isoforms remain the gold standard test for PCa detection. Many antibodies have been generated for the detection of PSA with good sensitivities and limits of detection. To ensure reliable assay performance a number of biomarkers …
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Isolation and Characterization of Anti-SLP Single Domain Antibodies for the Therapy of C. difficile Infection
… gastrointestinal infections in Canada. Current antiobiotic treatment is non-ideal due to the high incidence of relapse and the rise in hyper-virulent antibiotic-resistant strains. Surface layer proteins (SLPs) cover the entire bacterial surface and mediate adherence to host cells. Passive and …
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Adiponectin : a novel circulating anti-thrombotic factor in humans?
… inhibited by the glycoprotein VI (GPVI) blocking antibody, 10B12, but not ethylene glycol-bis (β-aminoethyl ether)-N,N,N’,N’,-tetraacetic acid (EGTA), suggesting a GPVI-dependent and integrin-independent mechanism.Together these data significantly improve our understanding of the mechanisms by …
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Suppressive effect of a nucleic acid-hydrolyzing recombinant antibody (3D8) on viral multiplication
… chain variable fragment (scFv) is a recombinant anti-DNA antibody from an autoimmune-prone MRL-lpr/lpr mouse with lupus diseases. 3D8 scFv can hydrolyze ss-DNA, ds-DNA, ss-RNA, and ds-RNA without sequence specificity. On the base of 3D8 scFv property, we carried out quantitative and qualitative …
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Characterization of Homogeneous and Heterogeneous Anti-Nucleic Acid Autoantibodies in Systemic Lupus Erythematosus
Three murine monoclonal anti-ssDNA autoantibodies were generated by fusion of spleen cells from autoimmune diseased NZB/NZW F1 hybrid mice with Sp 2/0-Ag 14 murine myeloma cell line. These monoclonal antibodies were purifed by affinity chromatography, and their immunochemical properties were …
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Antibody phage-displayed libraries derived from chicken immunoglobulin genes : a source of highly specific diagnostic antibodies
In meeting the high demand for monoclonal antibodies, the chicken immunoglobulin system was exploited to generate recombinant antibodies against multiple target antigens. Following simultaneous immunisation of two chickens with a mixture of Plasmodium falciparum recombinant lactate dehydrogenase …
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Multiplexed Mapping of the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins
… might be much more widespread than previously anticipated. However, potential direct interactions among the three known RAMPs (RAMP1, RAMP2 and RAMP3) and hundreds of GPCRs have never been investigated. To address this gap in knowledge about the GPCR-RAMP interactome, we developed a multiplexed …
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Toxic effects of dietary methylmercury on immune system structure, function, and development in the American kestrel (Falco sparverius)
… phytohemagglutinin (PHA) skin-swelling assay and antibody-mediated immune function using the sheep red blood cell (SRBC) hemagglutination assay. Hematology profiles were evaluated over time in adult males and nestlings to monitor immunological and physiological status of kestrels. Primary and …
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Selective Isolation of Circulating Tumor Cells in Antibody-Functionalized Microsystems
… microfluidic devices functionalized with proper antibodies was studied. Under static experimental conditions, microchambers were utilized to study the parameters such as cell suspension concentration, incubation time or ambient temperature that may affect the binding of cell to the functionalized …
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A Peptide-Based Platform for Displaying Antibodies to Engage T Cells
This study investigated a strategy by which antibodies were displayed on a gel-like substance to engage T cells. The substance, a peptidic composite, was characterized in vitro and explored as an injectable system in vivo. The composite consists of two amphiphilic peptides, AEAEAKAKAEAEAKAK …
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Anti-asthmatic and anti-cough activities of the essential oil Of Eucalyptus Grandis W. Hill Ex Maiden
… plant used by Zulu traditional healers for its antiasthmatic and anti-cough activities in the treatment of respiratory tract infections. The anti-asthmatic and anti-cough activities of the essential oils and 1, 8-cineole on rats were assessed. These activities were induced and challenged with …
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