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Showing 1 to 20 of 23 for “"anther culture"”.
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Embryogenic response of potato anther culture to colchicine
… potato, and if colchicine treatments during anther culture affected the ploidy of anther-derived plants. An additional study, analysis of genetic identity among regenerated anther-derived plants based on RAPD markers, was also conducted. Anther culture of a hybrid between <i>Solanum …
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Factors affecting variability in anther culture and in regeneration of androgenic embryos of Solanum phureja
The variation for embryo production in anther culture of Solanum phureja was examined as a function of maximum greenhouse temperature prior to bud harvest and innate responsiveness among anthers within a bud. S. phureja clones PP5, AD2-4, A3P2-6 and AD3-4 were grown in a greenhouse under a 16 h …
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Application of molecular markers to characterize potato plants derived from anther culture and protoplast fusion
… of the ploidy level can be realised through anther and microspore culture. This method bears a great deal of significance in that it provides a selection against lethal recessive genes. Monoploids carry only a single set of chromosomes and every locus carries only one allele. So the surviving …
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Bulk segregant analysis for anther culture response and leptine content in backcross families of diploid potato
… three traits in backcrosses. Two of the traits, anther culture competence and 2<I>n</I> pollen production, originated from <I>S. phureja</I> whereas the third, leptine production (a specific glycoalkaloid known to convey resistance to the Colorado potato beetle) originated from <I>S. …
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Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and correlations among them
Competence for leaf disc regeneration, anther culture, and protoplast culture was tested in the parental, F₁, and F₂ generations of a diploid cultivated primitive potato, S. phureja (2n=2x=24). The parental pair consisted of AM3-8, an anther culture derived homozygous diploid, and NBP2, a …
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Efficient production of doubled haploid plants through antimicrotubule agent treatment of anther-derived maize callus and the RFLP analysis of the maize anther culture response
… were used to induce chromosome doubling of maize anther-derived haploid callus before plant regeneration. Without treatment with these agents, all the plants regenerated from the callus were haploid. More than 50% of the plants regenerated from the callus treated with 0.625 or 1.25 uM colchicine …
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Studies of androgenic processes in diploid potato
… shock (35°C) during the first 12 h of anther culture and elevated incubation temperature (30°C 16h/20°C 8h) (hereafter 35°C-30°C/20°C) enhanced androgenic embryo production. Variation among experiment dates was highly significant. Temperature treatment (35°C-30°C/20°C) during anther …
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Molecular marker analysis of a segregating monoploid potato family
Anther culture experiments were conducted to construct a monoploid family. The donor plants used were hybrids between high leptine producing selections of Solanum chacoense Bitt. and anther culture responsive selections of Solanum phureja Juz. et Buk. Several steps of the anther culture process …
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Development of intermonoploid somatic hybrids of potato and their molecular analysis based on polymorphism for retroelement Tst1
… to hasten the process are available including anther culture to halve the genome and protoplast fusion to create hybrids between incompatible partners. We applied these techniques to potato to evaluate their potential for breeding highly heterozygous, cross-pollinating species. Four families of …
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Use of monoploid solanum phureja in cell and tissue culture techniques for potato improvement
Monoploid genotypes (2n = x = 12), derived by anther culture of a diplandrous genotype of Solanum phureja, a South-American diploid potato species, were examined for their utility in germplasm development. Nine monoploid genotypes and the diploid anther-donor plant were grown in photoperiod …
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Analysis of anther-derived plants of Solanum phureja: variation in ploidy, photosynthetic efficiency and structure of the nuclear genome
… of this research were to analyze variation among anther-derived plants of Solanum phureja regarding their: 1) ploidy level and morphology, 2) net photosynthesis and its biochemical components, and 3) nuclear genomic structure, particularly with regard ·to the amplification of rRNA genes as …
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Evaluation of anther-derived, inbred, and hybrid populations of Streptocarpus X hybridus Voss
… and selective hybridization, along with anther culture, for increasing homogeneity with 2 early flowering cultivars of Streptocarpus X hybridus was examined. A 'Concorde' and 'Mixed Nymph' genotype expressed inbreeding depression within 2 generations, characterized by a reduction in …
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Protoplast Fusion for the Production of Intermonoploid Somatic Hybrids in Cultivated Potato
… genotypes from a population of more than 100 anther-derived monoploids. Chemical fusion and electrofusion between pairs selected from 31 superior monoploids resulted in the production of three different groups of intermonoploid somatic hybrids. The hybridity of somatic hybrid plants and …
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Transposon Tagging in Strawberry and Potato and Characterization of Representative Strawberry Mutants
… were found in both T0 primary regenerates and anther culture derived potatoes using both the pAcDs-AtSCP and pAcDs-AmDEFH125 constructs. Sequencing of the empty donor site revealed that excision occurred in different cells during anther culture. A strawberry mutant with sugar transport …
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AFLP Marker Analysis Of Monoploid Potato
… monoploid populations derived by androgenesis in anther culture, and gynogenesis through the use of a haploid-inducing pollinator, S. phureja "IVP 101." Little indication of somaclonal variation and haploid-inducer gene introgression was found in the monoploid band data suggesting genomic …
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The Potential for Green Fluorescent Protein as a Screening Tool in the Production of Haploid Potato Plants
… microscopes and quantitatively with a GFP meter. Anther culture of tetraploids produced 29 plants, none with high levels of GFP. Segregation ratios for tetraploid T1 seedlings fit models for single duplex insertions (35 transgenic: 1 non) or double simplex insertions (15 transgenic: 1 non). …
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Derivation of interspecific Solanum hybrid genotypes with resistance to Colorado potato beetle (Leptinotarsa decemlineata Say)
The anther culture response of diploid Solanum chacoense (chc) - S. phureja (phu) hybrids and the regeneration potential of anther-derived monoploids was evaluated. In vivo evaluation of interspecific hybrids was also performed. Three hybrids were anther cultured to observe the effects of reduced …
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Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families
… = 12) can be derived either paternally through anther/microspore culture or maternally through crossing with a haploid-inducing pollinator. Evidence from other genera suggests that androgenic and gynogenic haploid populations derived from the same parent may differ due to gametic selection …
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Inheritance of protoplast culturability and improvement in pollen development by protoplast manipulation in solanum
… defined as the ability to develop calli from cultured protoplasts, was studied in the diploid potato species, Solanum phureja. Based upon data from F₂ as well as from F₁ and backcross progenies, it was found that protoplast culturability in this potato species was controlled by two unlinked …
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Derivation, fertility and breeding value of doubled monoploids from the diploid potato species, Solanum phureja
… monoploids (<i>2n = x = 12</i>) derived by anther culture of ten diploid clones of <i>Solanum phureja</i> were used to generate doubled monoploids through in vitro shoot regeneration. Doubled monoploids were compared to the anther donor and progenitor monoploids for morphological …
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