Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"ankyrin repeat"”.
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Structure/Function Studies on Vps9 domain-containing Ankyrin Repeat protein (VARP)
Vps-9 domain ankyrin repeat protein (Varp) is a widely expressed multi- domain protein of 1050 amino acids that resides in the cytosol. Varp has been shown to be a Rab21 GEF, a Rab32/38 effector and also helps to regulate VAMP7 fusion activity. Varp has more recently been proposed to bind to the …
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Identification and characterization of GRP50 : a novel Golgi-associated ankyrin repeat protein
… of a novel gene, GRP50 (Golgi-associated ankyrin repeat protein of 50 kilodaltons), which encodes a highly conserved mammalian protein exhibiting a unique structural architecture. Homology searching tools reveal that no known orthologues of this protein have been identified in other …
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Characterization of the vaccinia virus ankyrin repeat protein K1 on NF-κB inhibition and its contributions to viral pathogenesis
All viruses strategically alter the anti-viral immune response to their benefit. The goal of this thesis was to examine how the vaccinia virus (VACV) K1 protein, which is encoded by the K1L gene, modulates the immune response in vitro and in vivo. In vitro, the K1 protein has multiple …
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Characterization of Novel Substrates of the Tankyrase and RNF146 Destruction Complex and Mechanisms of its Regulation
… 1 and 2, whose domain organization include ankyrin repeat clusters which mediate enzyme-substrate interactions and a SAM domain which regulates oligomerization of Tankyrase into large macromolecular complexes. Tankyrases bind to proteins through a ‘RxxxxG’ peptide motif which facilities …
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Development of peptide inhibitors as molecular therapeutics against tandem-repeat proteins
Tandem-repeat (TR) domains occur in approximately one third of all proteins, yet these domains are less well understood compared with their globular counterparts. TR proteins act as molecular scaffolds that facilitate protein-protein interactions (PPIs) in diverse biological contexts. In disease …
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A study of Notch signalling in developmental angiogenesis
… endothelial stalk cell. The Notch-regulated ankyrin repeat protein, Nrarp, is a Notch target gene that is highly expressed in stalk cells and endothelial cells located at vessel branchpoints. Deletion of Nrarp in mouse and morpholino knockdown of nrarp-a and nrarp-b in zebrafish resulted in …
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Characterizing and engineering antibodies against the epidermal growth factor receptor
… of four different antibodies and three designed ankyrin repeat proteins binding to EGFR. In addition, we continued work on engineering novel antibodies against EGFR domains II and IV. Antibodies against these domains are hypothesized to directly inhibit receptor dimerization and subsequent …
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Characterization of the anti-viral effects inhibited by the vaccinia virus K1 protein
… that the C-terminal portion of the 2nd ankyrin repeat, a motif important for protein-protein interactions, is important for this inhibitory function. Further, K1 mediated PKR inhibition also blocks activation of downstream NF-κB in VV infected cells. Previous characterization of the K1 …
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Investigating the mechanisms and outcomes of Sprouty-2 S-acylation
… explored and although SPRY2 interacted with the ankyrin repeat (ANK) domain of zDHHC17 through a canonical mechanism, this mode of binding was dispensable for S-acylation. Binding assays suggested the presence of an additional lower affinity interaction site, which might involve amino acid …
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Analysis and Redesign of Protein-Protein Interactions: A Hotspot-Centric View
… interface between the synthetically evolved ankyrin repeat protein Off7 with E. coli maltose binding protein by characterization of mutant variants and redesigned interfaces. In order to characterize many mutants, I developed two high-throughput assays to measure protein-protein binding that …
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Elucidating TRPA1 Ion Channel Aberrations in Oesophageal Adenocarcinoma.
… identified TRPA1 (Transient Receptor Potential Ankyrin 1) as a novel driver gene in OAC with recurrent mutations in a sequenced cohort of 551 OAC patients. TRPA1 is a ligand-gated, Calcium (Ca2+) -permeable membrane protein, which has recently been implicated in breast and prostate cancers. This …
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Molecular Determinants of TRP Channel Gating
… structure: an intracellular N terminus featuring ankyrin repeats, six transmembrane helices including the pore domain, a re-entrant loop and TRP helix, and an intracellular C terminus with varying structure. Each of these regions has been implicated in the regular function of TRPs, while mutations …
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Functional analysis of ANKRD11 and FBXO31: two candidate tumour suppressor genes from the 16q24.3 breast cancer loss of heterozygosity region.
… for its function as a tumour suppressor. Ankyrin repeat domain 11 (ANKRD11) was found to be a novel p53-interacting protein which enhanced the transcriptional activity of p53. ANKRD11 expression in breast cancer cell lines was shown to be down-regulated when compared to ANKRD11 expression …
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Exploring multivalent technologies and strategies for directed protein evolution
… large (>109) naïve phage-libraries of Designed Ankyrin Repeat Proteins (DARPins). The resulting libraries were subjected to evolution campaigns and the evolved variants emerging from selection rounds, as well as intermediate states, analyzed to address the questions of whether differences in …
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Proteinkonjugationen durch Kupfer-abhängige Formylglycin-generierende Enzyme zur Generierung von Antikörper-Wirkstoff-Konjugaten
… of the model protein DARPin E01 (designed ankyrin repeat protein) or the single-chain antibody scFv425-Fc. First, the protein was functionalized with an azide by the aldehyde-specific HIPS ligation (Hydrazino-iso-Pictet-Spengler) and then loaded with a toxin by click reaction. The …
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Developing nanobodies to stabilise the tumour suppressor protein p16INK4a
… to name a few. p16 is composed of four ankyrin repeats and it has a very low thermodynamic and kinetic stability and rapidly unfolds even in the absence of denaturants. This low stability means that the protein is highly vulnerable to point mutations, which can result in functional …