Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"and RNA sequencing"”.
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Comprehensive evaluation of error correction methods for high-throughput sequencing data
The advent of DNA and RNA sequencing has significantly revolutionized the study of genomics and molecular biology. Development of high-throughput sequencing technologies have brought about a quick and cheaper way to sequence genomes. Different technologies use different underlying methods for …
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Investigating The Role of Cd109 In Pancreatic Ductal Adenocarcinoma
… of four patient derived PDAC cell populations and our data shows a cell surface receptor CD109 to be a common vulnerability, the biologic role of which in PDAC is yet unstudied and largely unknown. We hypothesized that CD109 expression provides PDAC cells with a survival advantage, and promotes …
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The design and statistical analysis of single-cell RNA-sequencing experiments
<p>Next-generation DNA- and RNA-sequencing (RNA-seq) technologies have expanded rapidly in both throughput and accuracy within the last decade. The momentum continues as emerging techniques become increasingly capable of profiling molecular content at the level of individual cells. One goal of this …
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Cloning and regulation of rat and Xenopus laevis hepatic 3-hydroxy-3-methylglutaryl Coenzyme A reductase
The cDNAs coding for rat and Xenopus laevis 3-hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) reductase have been cloned and sequenced. The rat liver HMG-CoA reductase cDNA clone contains one open reading frame coding for 887 amino acids. Its 106 nucleotide long 5$\sp\prime$-noncoding region contains …
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Modifikuotų nukleotidų taikymas plataus masto nukleorūgščių analizei /
… was was to investigate the properties and utility of oligonucleotide-tethered 2′,3′-dideoxyribonucleoside 5′-triphosphates (OTDDNs), with emphasis on labeling of DNA and cDNA molecules for high-throughput sequencing applications. It was demonstrated that OTDDNs are substrates for a …
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The utility of modified nucleotides for high-throughput nucleic acid analysis /
… was was to investigate the properties and utility of oligonucleotide-tethered 2′,3′-dideoxyribonucleoside 5′-triphosphates (OTDDNs), with emphasis on labeling of DNA and cDNA molecules for high-throughput sequencing applications. It was demonstrated that OTDDNs are substrates for a …
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A High-Throughput Approach to Uncover Novel Roles of APOBEC2, a Functional Orphan of the AID/APOBEC Family
… has implicated APOBEC2 in diverse organisms and cellular processes such as muscle biology (in Mus musculus), regeneration (in Danio rerio), and development (in Xenopus laevis). APOBEC2 has also been implicated in cancer. However the enzymatic activity, substrate or physiological target(s) of …
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The Generation of Alternative Transcripts as a Means of Regulating Phosphorylation in Sonic Hedgehog Medulloblastoma
… phosphorylation is critical in development and tumor progression; however, the promiscuity of kinases often limits the ability to regulate phosphorylation events selectively. Co- and post-transcriptional mechanisms, such as alternative transcription start site (ATSS) usage and alternative …
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The effect of age on human monocyte epigenetics and function
… undergoes a series of cellular, transcriptional and epigenomic changes. Using RRBS and RNA-Sequencing data collected from a group of 46 healthy participants across an age range, bioinformatics techniques were used to characterise the effect of age on the epigenome and transcriptome of immune …
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Characterization of Aryl Hydrocarbon receptor and Kruppel-Like-Factor-6 interaction
The aryl hydrocarbon receptor (AhR) is a ligand activated transcription factor which regulates toxic response to environmental pollutants, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Upon ligand activation, the AhR translocates to the nucleus, dissociates from its chaperone complex, and …
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Genomic Evolution of Chemoresistance In Triple-Negative Breast Cancer Delineated By Single Cell Sequencing
… that displays extensive intratumor heterogeneity and frequently (46%) develops resistance to neoadjuvant chemotherapy (NAC). Currently, the genomic basis of chemoresistance remains poorly understood. An important question is whether resistance to chemotherapy is driven by the selection of rare …
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Viengrandines mažąsias RNR modifikuojančių gyvūnų Hen1 metiltransferazių taikymas RNR žymėjimui ir sekoskaitai /
… of the N-terminal methyltransferase domain and the variable-length C-terminal region, and catalyze the S-adenosyl-L-methionine (AdoMet) dependent methylation of the 2'-O-ribose of the 3'-terminal nucleotide in a subset of small non-coding RNAs. This modification protects small RNAs from …
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Genetic regulation of maize floral development - insights from the transcriptome and translatome
… plants is regulated by gene expression. Understanding gene expression regulation in maize floral development is critical to regulate floret fertility in other grasses and potentially useful to engineer more productive cereal crops. In this work, I focus on gene expression regulation at …
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Targeting neurodegenerative phenotypes and mitochondrial dysfunction in a Drosophila Melanogaster model of Parkinson’s Disease
… to motor symptoms such as tremor, rigidity, and bradykinesia, as well as non-motor symptoms includes mood disorders, sleep disturbances, and autonomic dysfunction. Mitochondrial dysfunction, particularly involving Complex I of the electron transport system and mitophagy pathways, is …
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The Modulation of Innate Immunity by Cellular and Viral Noncoding RNAs
Growing evidence demonstrates that endogenous and viral non-coding RNAs possess the ability to alter innate immunity and inflammatory responses. For example, endogenous non-coding RNAs can be recognized by pattern recognition receptors to trigger innate immune activation and inflammation. Many …
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Using Mouse Models to Define How The P53 R72P Polymorphism Impacts The Adverse Effects of Doxorubicin and Ionizing Radiation
… to genotoxic insult. Studies in cell culture and in tissues from mouse models of the SNP indicate that, in response to gentoxic treatment, the two variants may differentially induce apoptosis and expression of p53 target genes. In epidemiological studies, the P variant is associated with …
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Multiple virulence genes and other MRNA regulatory targets are differentially expressed in two Helicobacter pylori strains overexpressing small RNAs
… infecting the stomachs of half of humanity and the first bacterium listed as a carcinogen. Strains possessing the cytotoxin-associated gene pathogenicity island (cagPAI) are especially likely to cause symptomatic disease and cancer through the action of the cagPAI Type IV secretion system …
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The impact of acute heat exposure on the heat shock response (HSR) between males and females
… (EUM) females in the mid-luteal phase (n=10), and combined monophasic oral contraceptive (OCP) user females (n=9) who underwent a 20-min far-infrared (IR) sauna session (57.2 ± 2.0ºC, 24.7 ± 5.6% relative humidity). Core temperature, skin temperature, heart rate, and thermal perception were …
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Transcriptional regulation of adipose insulin resistance
… is a condition that underlies type 2 diabetes and various cardiovascular diseases. It is highly associated with obesity, making it a pressing medical problem in face of the obesity epidemic. The obesity association also makes adipose tissue the target of interest for ongoing research. Previous …
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Systems Analysis of Plant Responses to Drought
Understanding how plants respond to environmental stress is critical for ensuring stable crop performance and predicting how natural populations may adapt to a changing climate. While plant biology has traditionally focused on plant physiology and molecular biology of model plants to elucidate …
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