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Showing 1 to 20 of 42 for “"anaphase-promoting complex"”.
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Regulation of Anaphase Promoting Complex Coactivators
… From late mitosis through early G1, the Anaphase Promoting Complex (APC) is essential for cell-cycle relevant proteolytic degradation, and its activity is targeted to appropriate substrates by the evolutionarily conserved coactivators Cdc20 and Cdh1. After an initial wave of APC-Cdc20 …
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Meiosis-specific Regulation of the Anaphase-Promoting Complex
… are triggered by an ubiquitin ligase called the Anaphase-Promoting Complex or Cyclosome (APC/C). APC/C-dependent destruction of securin/Pds1 is required to activate separase, a thiol protease that mediates chromosome segregation by cleavage of the cohesin complex. The first meiotic division is …
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Harnessing the Anaphase-Promoting Complex for Targeted Protein Degradation
… is yet to be explored in the field, namely the anaphase-promoting complex or cyclosome (APC/C). The APC/C is a vital ubiquitin ligase involved in the destruction of numerous proteins including Securin, Cyclin B1 and Aurora kinase A. Most natural APC/C substrates contain multiple copies of short …
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INSIGHTS INTO SUBSTRATE RECOGNITION BY THE ANAPHASE PROMOTING COMPLEX (APC)
… is executed by an E3 ubiquitin ligase called the anaphase promoting complex (APC). APC adds polyubiquitin chains to numerous substrates, which leads to their subsequent degradation in late mitosis and G1 phase. The selective and timely recognition and degradation of APC substrates is essential for …
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Regulation of Anaphase Promoting Complex/Cyclosome to Control M Phase Exit
<p>The Anaphase Promoting Complex/Cyclosome (APC/C) is a RING E3 ligase that plays essential roles both within and outside of the cell cycle. At the onset of anaphase, the APC/C targets cyclin B and securin for degradation, initiating chromosome separation and mitotic exit. Regulation of APC/C …
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Regulation of the Anaphase-Promoting Complex Examined at the Single Cell Level
… degradation, mediated by ubiquitylation by the anaphase-promoting complex (APC). In this work, I analyzed the regulation of the APC by its activator Cdh1 in budding yeast in single cells. Inactivation of APC-Cdh1 is an important regulatory transition leading to mitotic entry. I developed and …
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Understanding Structural And Functional Mechanisms of Emi1 Inhibition of the Anaphase Promoting Complex
… the cell cycle. A mitotic regulator called the Anaphase Promoting Complex, also called Cyclosome (APC/C), modifies myriad substrates that control cell cycle activities. The APC/C is a ubiquitin ligase complex; it is the final enzyme in a tri-enzyme cascade and catalyzes the final step of Ub …
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The developmental role and regulation of the Anaphase-Promoting Complex/Cyclosome in Drosophila meiosis
… driven in part by proteolysis mediated by the Anaphase-Promoting Complex/ Cyclosome (APC/C). During oogenesis of multicellular organisms, molecular control of meiosis has an added complexity because these divisions must be coordinated with the growth and development of the oocyte as well as its …
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A meiosis-specific form of the Anaphase Promoting Complex/Cyclosome regulates the oocyte-to-embryo transition in Drosophila
… irreversible manner. At the heart of this is the Anaphase Promoting Complex/Cyclosome (APC/C), an E3 ubiquitin ligase. The APC/C targets its substrates for degradation, and thus progresses the cell cycle irreversibly forward. Many substrates of the APC/C have been identified in mitosis, but how …
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Design and development of novel antagonists targeting Cdc20, a central regulator of the Anaphase-Promoting Complex/Cyclosome, for the treatment of cancer
… SAC regulator and mitotic protein, binds the Anaphase-Promoting Complex/Cyclosome (APC/C), a complex capable of stipulating protein destruction, regulating its activity and driving cell cycle progression through orderly APC/C substrate degradation. Key oncogenic roles and aberrant expression …
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Morphological and Neuroanatomical Changes Associated with the Induction of Neurodegeneration and Life Span Reduction in Drosophila melanogaster by Glia Targeted Expression of Cdh1/Rap/Fzr, An Activator of the Anaphase Promoting Complex
… (Fzr), an activator of the E3 ubiquitin ligase complex: the Anaphase Promoting Complex/Cyclosome (APC/C). APC/C facilitates the cell cycle stage-specific degradation of cyclins and other mitotic regulators, resulting in the inactivation of Cdk (Cyclin Dependent Kinase) complexes, thus promoting …
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Strengths and Weaknesses of Hybrid TPR Technology for Obtaining Structural and Mechanistic Insights into TPR Proteins
… often through a central groove. One protein complex bearing numerous TPR repeats is the Anaphase Promoting Complex (APC). The anaphase-promoting complex (APC) is a multi-subunit complex, which orchestrates mitotic cell cycles. APC is an E3 ligase in the ubiquitin cascade, and directs the 26S …
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Regulation of the spindle checkpoint by Mad2 binding proteins
… fidelity of chromosome segregation by delaying anaphase until all sister chromatids form proper bipolar attachments to the mitotic spindle. Spindle checkpoint proteins localize to unattached or maloriented kinetochores in mitosis and generate a signal that prevents dissolution of sister …
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Histone H3 K4 Methylation Regulates The Spindle Assembly Checkpoint Through Direct Binding of Multiple Checkpoint Components and Cdc20
… Bub3 and Mad2 as well as the activator of the Anaphase Promoting Complex (APC) protein Cdc20. <em>MAD2</em> encodes a HORMA domain, which I identify as a novel histone binding motif. Mad2 maintains two differing conformations, closed Mad2 (C-Mad2) and open Mad2 (O-Mad2). We find C-Mad2 …
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Enhanced protein degradation by branched ubiquitin chains
… roles of branched structures are unknown. The anaphase-promoting complex (APC/C) catalyzes degradation of key regulators during the cell cycle causing proper transition through the cell cycle. Here, I report that the APC/C efficiently synthesizes substrate-attached branched conjugates and …
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Role of Drosophila Rap/Fzr (Cdh1) in retinal axon targeting and its interactions with Loco, Liprin-a and Ras
… is a homolog of mammalian Cdh1, an activator of anaphase promoting complex (APC), an E3 ubiquitin ligase. Previous work has shown that Rap/Fzr is required in cell cycle regulation, glia differentiation and pattern formation during eye development. Results from our experiments show that Rap/Fzr is …
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Mitotic regulators and their effects on Drosophila : chromosome structure during development
… in Drosophila nurse cells, is a subunit of the anaphase-promoting complex/cyclosome. Increasing levels of cyclin B, a known mitotic target of the APC/C, does not alter the timing of the transition, indicating that CYCLIN B is not the only APC/C target at the polyteny-polyploidy transition. In …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… (SAC), activates the mitotic checkpoint complex (MCC), to inhibit the E3 ubiquitin ligase, the anaphase-promoting complex/cyclosome (APC/C). Inhibition of the APC/C then delays premature chromosome segregation by preventing APC/C-mediated degradation of two key cell cycle regulators, …
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Cyclin B1 Mediates The Effect of Uchl1 In Promoting Cell Cycle Progression In Uterine Papillary Serous Carcinoma
… the ubiquitination of cyclin B1 by the anaphase-promoting complex. Accordingly, UCHL1 silencing slowed the progression of cells into mitosis. Taken together, our findings indicate that UCHL1 impairs the degradation of cyclin B1, leading to uncontrolled cell cycle progression. In summary, …
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