Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"analytical ultracentrifugation"”.
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Probing metal nanoparticles and assemblies with analytical ultracentrifugation
Analytical Ultracentrifugation (AUC) is a powerful tool to obtain statistically relevant size and shape measurements for macromolecular systems. Metal nanoparticles coated by a ligand shell of thiolated molecules provide diverse functionality, from targeted cellular delivery to the formation of …
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Identifying novel proteins in translation complex by using analytical ultracentrifugation with fluorescent detection system
… are in the translation complex, we applied analytical ultracentrifugation with fluorescent detection system (AU-FDS) to detect this complex. Following a one-step affinity purification with Flag-PAB1 using strains carrying translation factors and specific mRNA tagged with GFP, we identified …
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Nonideality in high concentration solutions
… energy framework was sufficient to explain analytical ultracentrifugation data gathered in complex biological solutions. Additional hypotheses tested were as follows: The Fluorescence Detection System for the Analytical Ultracentrifuge will enable the tracking of a single component is a …
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Structural Characterization of the C-terminus of the Cochlear Motor Protein Prestin: Intrinsic Disorder Linked to Voltage Sensor Function
… magnetic resonance spectroscopy (NMR), and analytical ultracentrifugation were used to characterize the folding and stability of the deletion mutants. </p> <p> The results showed that the deletion mutants existed as folded, monomeric proteins in solution. Interestingly, several of the …
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A system for automating the interpretation of Analytical Ultracentrifuge data
… to acquisition, sharing, and analysis of Analytical Ultracentrifugation (AU) data must be made. XML data formats have been defined to allow complete exchange of the information associated with an AU experiment. Extensible formats to record solution identity, instrument setup parameters, …
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Analysis of the multiple chaperonins of Mycobacterium smegmatis
… temperatures (42°C). Using native gels and analytical ultracentrifugation, purified Cpn60.2 did not oligomerise under normal conditions, however in the presence of nucleotide and high concentrations of salt, the formation of large oligomers was observed. Neither Cpn60.1 nor Cpn60.3 …
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The Rev-mediated Dimerization of HIV RRE RNA
… Using size-exclusion chromatography (SEC), analytical ultracentrifugation (AUC), and small angle X-ray scattering (SAXS), I demonstrate the formation of a discrete Rev:RRE RNA complex. Data analysis presented in this work suggests that Rev is mediating a dimer of RRE RNA.
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The Rev-mediated Dimerization of HIV RRE RNA
… Using size-exclusion chromatography (SEC), analytical ultracentrifugation (AUC), and small angle X-ray scattering (SAXS), I demonstrate the formation of a discrete Rev:RRE RNA complex. Data analysis presented in this work suggests that Rev is mediating a dimer of RRE RNA.
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Functional and structural characterisation of Mycobacterial Chaperonins
… that they function in a similar manner. Using analytical ultracentrifugation on purified proteins, I have been able to show that Cpn60.2 and its chimeric protein do form larger oligomeric complexes in vitro under certain conditions and in the presence of ATP.
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Applications of fluorescence detected sedimentation I Studying the thermodynamic impact of valence in non-ideal solutions II High affinity interactions and biological media
… and interference optical systems on the analytical ultracentrifuge has greatly expanded the wide application of this technique. The new FDS is able to detect very low concentrations of fluorophore allowing the study of high affinity interactions (Kd ∼ pM) not previously accessible …
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Tuning polymeric chiral surfactants for enhanced enantiomeric recognition in micellar electrokinetic chromatography
… by use fluorescence, PFG-NMR, densitometry, and analytical ultracentrifugation (AUC). The partial specific volumes of the fractionated and non-fractionated surfactant were determined by use of a densitometer. The molecular weights were determined by use of sedimentation equilibrium mode in AUC. …
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Structural and spectroscopic studies on the porin-cytochrome complex from Shewanella oneidensis MR-1
… this"protein" were" investigated" using" analytical" ultracentrifugation" (AUC)," small" angle" X!ray" scattering" (SAXS)" and" spectropotentiometric" techniques." MtrA" and" a" truncated"N!terminal" MtrA" construct"containing" the" five"N!terminal"hemes"(MtrA"N)"were"both"observed" …
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BIOCHEMICAL CHARACTERIZATION OF SUPPRESSOR OF IKK-ε AND NAK-ASSOCIATED PROTEIN 1
… by size exclusion chromatography and analytical ultracentrifugation. Hetero-interactions were explored via co-precipitation assays of recombinant proteins. SIKE 72 formed a primarily dimeric structure whereas NAP 255 forms a single species that appears to be monomeric at this stage of …
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Proteins Inside Cells and On the Surface of Membranes: Developing Three Dimensional In-Cell NMR and Structural Characterization of a Trimeric Membrane Proximal External Region Construct from HIV-1 gp41
… antibodies 2F5 and 4E10, as shown by equilibrium analytical ultracentrifugation and surface plasmon resonance. Finally, we have solved the structure of this construct bound to detergent micelles using NMR spectroscopy. The structure shows that the MPER adopts an α-helix conformation and suggests …
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Structural and Functional Studies of the Munc13 MUN Domain and the RIM C2B Domain
… C2B domain homodimer, which was confirmed by analytical ultracentrifugation and NMR spectroscopy. Our results suggested a model that RIM1 C2B dimerization might facilitate the Munc13 C2A homodimer to Munc13 C2A/RIM zinc finger heterodimer switch during synaptic vesicle priming.
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Identification and stoichiometric analysis of the monosomal translational complex
… purification, using a novel technique of analytical ultracentrifugation with a fluorescence detection system (AU-FDS) I have identified a 77S monosomal translational complex in the yeast Saccharomyces cerevisiae. Major components of the 77S complex include the 80S ribosome, mRNA, and …
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The identification and characterization of the interaction between Upf1 and PAB1 during nonsense-mediated decay & the identification of novel protein complexes associated with translation termination factor eRF1
… was performed using the novel technique of analytical ultracentrifugation with a fluorescence detection system (AU-FDS) on Flag-eRF1 affinity purified extracts. AU-FDS revealed that Flag-eRF1 associates with six distinct complexes that have S values of 20S, 28S, 39S, 57S, 77S, as well as …
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Regulation of Histone Covalent Modifications During Yeast Apoptosis
… fold into compacted conformation as measured by analytical ultracentrifugation. Moreover, an interaction between the forkhead homology-associated domain 1 (FHA1) of Rad53 and H2BS10ph was uncovered. This interaction inactivates the DNA damage checkpoint pathway and promotes apoptotic chromatin …
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Solution Conformations and Structural Thermodynamics of Type I DNA Polymerases from Escherichia coli and Thermus aquaticus
… been examined in solution using hydrodynamic (analytical ultracentrifugation) and small/wide angle X-ray scattering techniques and then compared to their known crystal structures to assess the similarities and differences in the overall structure of these enzymes and enzyme complexes within a …
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