Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"amyloids"”.
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The Role of Bacterial Amyloids In Regulating Gastrointestinal Homeostasis
… multicellular communities known as biofilms. Amyloids, proteins with a conserved β-sheet quarternary structure, show high resistance to many chemical and enzymatic processes including proteinase K and SDS treatments and are produced as essential adhesins during biofilm formation. Curli fibers …
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Application of Functional Amyloids in Morphological Control and in Self-assembled Composites
Amyloids are self-assembled protein materials containing beta-sheets. While most studies focus on amyloids as the pathogen in neurodegenerative disease, there are instances of "functional" amyloids used to preserve life. Functional amyloids serve as an inspiration in materials design. In this …
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Structural studies of the in vitro assembly of tau and α-synuclein amyloids
… protein aggregates, which are composed of amyloids. Cryo-EM studies of amyloid filaments isolated from human brains have revealed that specific conformers of tau and α-synuclein are associated with different diseases. These findings suggest that specific molecular mechanisms underlie the …
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Structure, conformational dynamics and formation of large amyloids: the case of alpha-synuclein fibrils
In my thesis, I have focused on new methodology development combined with state-of-the-art solid-state nuclear magnetic resonance (NMR) experiments with scanning transmission electron microscopy (STEM) to obtain atomic level structural information of the alpha-synuclein (AS) fibrils and the …
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Kinetics of Peptide Aggregation
… mechanism to protect or destroy life. Pathogenic amyloids are the signature of neurological disorders such as Alzheimer's and Parkinson's disease and bovine spongiform encephalopathy (BSE), which have no effective treatments or known cures. Pathogenic amyloids appear as nanometer sized "plaques" …
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Methods for study and manipulation of protein self-assembly
… properties. Their disease association makes amyloids relevant for medical research, but the characteristic structure and associated properties have also led to an interest in harnessing them as building blocks for functional materials. This thesis presents the development of methods for the …
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Enhancement Of Ebola Virus Infection By Seminal Amyloid Fibrils
… with the type species of the seminal amyloids, SEVI. When present, SEVI resulted in a nearly 20-fold increase in infection of physiologically relevant target cells. SEVI and other seminal amyloids were also found to enhance authentic Ebola virus infection by 20- to 40-fold in cell …
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Prediction of parallel in-register amyloidogenic beta-structures In highly beta-rich protein sequences by pairwise propensity analysis
Amyloids and prion proteins are clinically and biologically important beta-structures, whose supersecondary structures are difficult to determine by standard experimental or computational means. In addition, significant conformational heterogeneity is known or suspected to exist in many amyloid …
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Probing the conformational dynamics of amyloid oligomers in biologically relevant microenvironments
… phenomenon remains poorly understood. While some amyloids are cytotoxic, others do not spontaneously aggregate in ways related to disease progression. Despite all amyloids sharing a propensity to form fibrils, the biophysical properties underpinning this distinction remain unclear. Additionally, …
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The role of buried stack residues in the folding of the beta-helix domain of P22 tailspike
… exhibits a cross-[beta] structure reminiscent of amyloids. To investigate the sequence requirements of these stacks for directing a polypeptide chain into a [beta]-helical fold, we performed systematic mutational studies using the complete P22 tailspike protein, which contains a 13 rung …
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Plasmacytoid Dendritic Cell-Mediated Humoral Autoimmunity
… <p>The immunization of DNA-containing amyloids into non-autoimmune mice triggers the activation of pDCs and induction of lupus-like disease, characterized by the production of autoantibodies. Using this lupus model that is dependent on pDC activation and IFN-I production, we delineated …
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Deciphering the role of Hsp31 as a multitasking chaperone
Among different type of protein aggregation, amyloids are biochemically well characterized state of protein aggregation that is commonly associated with a large number of neurodegenerative diseases in mammals and cause heritable traits in Saccharomyces cerevisiae. Among many other neurodegenerative …
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An investigation into the effects of inorganic element status on the accumulation and deposition of amyloid in various diseases.
… of Cu2+ Zn2+ C2+ and Αl3+ (>50 μM) to the amyloids, particularly Αβ(1-40) and β2M, showed some changes with a general decrease in a helix secondary structure and an increase in β sheets. Both the Αβ(l-40) and β2M peptides treated with physiological concentration of either Α13+, Ca2+ Cu2+ or …
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Plant proteins as multifunctional additives in polymer composites
… self-assembled into nanostructures called amyloids. Amyloids have the potential for high rigidity and stability due to high β-sheet content. In Chapter II, trypsin hydrolyzed wheat gluten (THWG) proteins were incubated in aqueous PVA solutions, then the composite solutions were air dried …
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Thermodynamic signatures for hexapeptides with propensity for phase separation and amyloid formation
… proteins. Some IDPs can also aggregate and form amyloids. The occurrence of these amyloids in vivo is a hallmark of various neurodegenerative disorders. Phase separation and amyloid formation are collective behaviour properties, which may be encoded by the primary structure (sequence) of the …
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Modeling of Therapeutic Drugs, Polymers, and Self-Assembling Peptides
… we studied a hypothetical coupling between amyloids and Spike proteins of SARS-CoV-2. C) We explored materials based on peptides self-assembled into porous crystals. We examined how mutations introduced in these peptides affect the stability of the observed modified crystals. We also …
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Development of the new yeast-based assays for prion properties
… suggested that the ability to form transmissible amyloids (prions) is widespread among yeast proteins and is likely intrinsic to proteins from other organisms. However, the distribution of yeast prions in natural conditions is not yet clear, which prevents us from understanding the relationship …
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Size-dependent mechanical properties of beta-structures in protein materials
… in strong protein materials such as silks, amyloids, muscle fibers and virulence factors. The biological recipe for creating strong, tough materials from weak bonds, however, has so far remained a secret. In this dissertation, size, geometry and deformation rate dependent properties of …
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Mechanistic Investigation into the Regulation of Amyloid Motifs in Tau Aggregation and Disease
… mechanism of how they promote tau assembly into amyloids is poorly understood. Many disease-associated mutations localize within tau's repeat domain proximal to amyloidogenic sequences, such as 306VQIVYK311. We use computational modeling, recombinant protein and synthetic peptide systems, …
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The Effect of Traumatic Brain Injury On Tau Pathology By A Potential Seeding Mechanism
… having amyloidogenic properties. Formation of amyloids, such as tau aggregates, is proposed to follow a nucleation-polymerization model where misfolded, soluble oligomeric seeds can trigger native proteins to misfold and aggregate. The processes responsible for the initial formation of seeding …
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