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Showing 1 to 20 of 32 for “"amplified fragment length polymorphism"”.
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Amplified fragment length polymorphism (AFLP) analysis of genetic variability in Phalaenopsis
Amplified fragment length polymorphism (AFLP) markers allow a rapid assessment of the level of genetic variation that would be difficult to evaluate using a limited number of morphological markers. AFLP was used to assess the level of genetic variation among 16 different Phalaenopsis species and …
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Genetic fingerprints of microalgal culture strains: amplified fragment length polymorphism (AFLP) for investigations below the species level
… genetischen Fingerabdrücken, die mit der AFLP (amplified fragment length polymorphism) Technik generiert wurden, untersucht. Mittels AFLP was es möglich, verschiedene Stämme derselben Art (Chlorella vulgaris) zu unterscheiden und für jedes Isolat einen charakteristischen genetischen …
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Introgression of the Genes for Sesquiterpene Carboxylic Acid Synthesis From a Wild Tomato, Lycopersicon Hirsutum, Into the Cultivated Tomato for Increased Host -Plant Resistance to Insect Pests
… of polymorphic molecular markers using the Amplified Fragment Length Polymorphism (AFLP) protocol. Progeny of each generation were separated into high and low classes for SCA levels and the DNA combined of individual plants within the phenotypic classes. Bulk segregant analysis was used to …
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Hybridization Between the Watersnakes <i>Nerodia sipedon</i> and <i>Nerodia fasciata,</i> in the Carolinas: A Morphological and Molecular Approach
… nuclear markers, identified by the AFLP (Amplified Fragment Length Polymorphism) technique were nearly fixed and served as diagnostic markers. They revealed extensive introgression between the two species not recognizable morphologically. Markers for <em>N. sipedon</em> and F1 hybrids, …
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Assessment of Genetic Variation of Acer rubrum L. and Liriodendron tulipifera L. Populations in Unmanaged Forests of the Southeast United States
… important trees. This study utilizes amplified fragment length polymorphism to determine the level of genetic diversity of these species in contrasting physiographic provinces. AFLP was performed using five primer combinations on samples collected from six unmanaged populations of each …
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Genetic Diversity in Native and Invasive <i>Rubus </i>(Rosaceae)
… genetic fingerprinting techniques were used; Amplified Fragment Length Polymorphism (AFLP) and Randomly Amplified Fingerprints (RAF). Using three AFLP primers and two RAF primers, genetic similarity was determined and phylograms were constructed. Through statistical analysis and phylogram data …
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Ecological genetics of the moss Physcomitrella patens
… of the plants collected was conducted using amplified fragment length polymorphism (AFLP) analysis. No spatial genetic structure was found within populations of P. patens, and it is hypothesised that the nature of the ephemeral aquatic habitats that P. patens occupies may account for this …
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Genética e mapeamento molecular da resistência parcial à ferrugem da folha da aveia (Avena sativa L.)
… Mapeamento molecular, com marcadores AFLP (amplified fragment length polymorphism), foi realizado em F2 e F6, do primeiro cruzamento, identificando marcadores associados à resistência quantitativa (“quantitative resistance loci” ou QRLs). Resultados de três anos evidenciaram alta influência …
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Approach to the identification of sex-determining genes in the tilapia genome by genetic mapping and comparative positional cloning
… genes, and will need further study. AFLP (amplified fragment length polymorphism)/BSA (Bulked segregant analysis) technique was performed to add more markers in the sex-determining region in O. niloticus. This method appeared to be not so efficient in this study, because, although 3 of 128 …
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Phenotypic and genotypic characterization of Thalassiosira pseudonana (Bacillariophyceae) strains
… that the strains were identical; however, Amplified Fragment Length Polymorphism (AFLP) analyses indicated that the axenic strains belonged to three different clusters. Although the population variability<br/>obtained was low, it was enough to group the strains according to their …
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Molecular phylogeny of Melanospora and Diaporthales, and population genetics of dogwood anthracnose fungus
… from the eastern U. S., were identified with amplified fragment length polymorphism (AFLP) markers and sequences of several genes. Discula destructiva is thought to have been introduced to North America in late 1970s. The remarkably low genetic diversity compared to other asexual fungi …
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The Contribution of Within-Field Inoculum Sources of Gibberella zeae to Fusarium Head Blight in Winter Wheat and Barley
… within-field and background sources. Using amplified fragment length polymorphism, released clones of G. zeae were tracked within wheat and barley fields. This strategy allowed the distinction between the contributions of released clones to FHB, compared to that of background inocula. Corn …
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Genome analysis of Campylobacter concisus strains from patients with inflammatory bowel disease and gastroenteritis
… studies using a number of techniques including amplified fragment length polymorphism (AFLP), analysis of housekeeping genes and 23S rRNA gene. A number of studies have reported C. concisus strains of GS2 to invade human epithelial cell line Caco2, and suggested that C. concisus strains of the …
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Genetic linkage map of LCP 85-384, genetic diversity of a S. spontaneum collection and the contribution of S. spontaneum to Louisiana commerical germplasm
Sequence related amplification polymorphism (SRAP) marker technique was used to assess genetic relationships and diversity among genotypes of Saccharum and allied genera. In the SRAP technique, the primers were arbitrarily designed with an AT- and GC-rich motif to anneal introns and exons, …
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Gene discovery: Polymorphism association with obsessive-compulsive disorder and proteome analysis of Artemisia annua.
PART I: Polymorphism association with Obsessive-Compulsive Disorder In light of the putative role of the serotonergic, dopaminergic and possibly (nor)adrenergic system in OCD, following polymorphisms were analysed in a sample of >100 OCD patients and a control sample of >100 ethnically matched …
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The effects of flooding disturbance on the distribution and behaviour of riparian arthropods along a lowland gravel river
… of flood-avoiding behaviour. By using Amplified Fragment Length Polymorphism (AFLP) markers, the genetic population structure of the highly stenotopic wolf spider Pardosa agricola (Thorell, 1856) was studied. Nearby populations were less differentiated genetically than populations on …
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Population Structure, Aggressiveness, and Molecular Genetic Variability of Sclerotinia Sclerotiorum
… 48 S. sclerotiorum isolates was assessed by amplified fragment length polymorphism (AFLP). A dendrogram was calculated utilizing the neighbor joining method within the NTSYS-pc system. Each isolate produced a unique AFLP genotype, and the range in genetic similarity was 0.1022 to 0.3387. AFLP …
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Development of intermonoploid somatic hybrids of potato and their molecular analysis based on polymorphism for retroelement Tst1
… and plant regeneration. Sequence specific amplified polymorphism (S-SAP) represents a hybrid system incorporating amplified fragment length polymorphism (AFLP) technology in conjunction with the use of a defined genomic sequence, e.g., retrotransposon display (RD) when the defined sequence …
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Characterization of the soybean genome in regions surrounding two loci for resistance to soybean mosaic virus
… which combined bulk segregant analysis with Amplified Fragment Length Polymorphism (AFLP). A Rsv4-linked marker, R4-1, was identified that mapped to soybean linkage group D1b using a reference mapping population. More than 40 markers were mapped in the Rsv4 segregating population including …
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Toward genetic marker-assisted improvement of aquaculture stocks
… of microsatellite loci or RAPDs (random amplified polymorphic DNAs). Experimental power calculations suggest that because markers will have to be screened within large progeny groups, selective or sequential genotyping or screening of bulked DNA samples will be needed for costeffective …
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