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Showing 1 to 20 of 48 for “"amount of protein"”.
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Analysis and optimization of immune responses elicited by Outer Membrane Vesicles (OMVs)
… peptidoglycan, lipopolysaccharide (LPS), and lipoproteins and can be decorated with foreign antigens. Thanks to these properties OMVs are becoming an attractive vaccine platform. However, a systematic investigation on the potentiality of OMVs to design vaccines to elicit protective …
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Plasma proteins and cardiac oedema
One of the factors in the causation of cardiac oedema is a depletion of the plasma proteins which results in a fall in the colloid osmotic pressure of the blood. This depletion is due principally to malnutrition, and it is suggested that cases of cardiac oedema should be given diets containing the …
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Engineering Soluble, High Affinity Receptor Antagonists for Bacterial Exotoxins
Chapter four describes the engineering of murine Vbeta8.2 for picomolar affinity to staphylococcal enterotoxin B (SEB). As the known contact regions had already been heavily mutagenized, additional engineering of the Vbeta was performed through extension of the CDR1 loop. Soluble forms of the …
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The Effect of Stability and Surface Charge Distribution on Secretion of Bovine Pancreatic Trypsin Inhibitor From Yeast
Biophysical characterization of the BPTI mutants shows that removal of charges results in a drop in the effective number of ionic binding sites, as analyzed by binding of the molecule to a cation exchange column. Removal of the charge on the uncharged face of the molecule results in a dramatic …
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The effect of fire and heat on germination of southeastern United States wildlife food plants with particular emphasis on the genus Cassia
… southeastern United States to promote the growth of legume plants which produce seeds important as quail food. These seeds are characterized by a hard seed coat and the ability to remain dormant but viable for long periods of time. This study was conducted to determine the effect of heat on …
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Assay and Purification of Coxsackievirus B3
The problem. Which of the available methodologies for the purification for Coxsackie virus B3 (CB3), a Picornavirus provide the highest degree of purity with the greatest yield. The procedure. Quantitative methods for the assay and purification of CB3 were developed. CB3 was propagated in buffalo …
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Developmental changes in nitrogen and protein of tall and dwarf tomato seedlings Lycopersicon esculentum Mill
Tris-soluble protein and total nitrogen of tall and dwarf tomato (Lycopersicon esculentum Mill.) seedlings were determined by the method of Lowry et al. and a modified micro-Kjeldahl direct Nesslerization technique. The tall tomato 'Fireball' differs from the dwarf 'Md. 412-4' in several ways: (1) …
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Determinants of translational efficiency in Saccharomyces cerevisiae
The goal of this thesis is to elucidate the mechanisms that govern translational efficiency (TE) - the amount of protein produced from each molecule of mRNA. While the mechanisms regulating the TE of a few specific messages are well understood, the general contribution of translational control to …
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The effect of Moringa Oleifera leaves and termite (Isoptera) powders on nutritional and sensory properties of an instant-maize porridge
… countries contributing significantly to the diet of rural populations in developing countries. White maize is deficient in some amino acids and over-dependency on its porridge may lead to high prevalence of malnutrition-related health conditions. Moringa oleifera (MO) and termite (Isoptera spp.) …
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ENHANCEMENT OF hFVIII ACTIVITY THROUGH LC MODIFICATIONS FOR GENE THERAPY OF HEMOPHILIA A
… the recombinant Adeno-associated virus (rAAV) offers an alternative to classic treatment, which consists of FVIII protein infusions. However, due to limitations associated with rAAV and the FVIII protein itself, the end result is a transgene expression below therapeutic limits. One approach to …
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Cut-off values and thresholds of allergic reaction during oral food challenge: a retrospective study
Food allergy (FA) negatively impacts the quality of life of millions of people and represents a substantial physical and economic burden for the patient, his family, and the state. The gold standard for food allergy diagnosis worldwide is the oral food challenge (OFC). However, data on the OFC …
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Gene amplification in Drosophila ovarian follicle cells as a developmental strategy and model for metazoan DNA replication
The process of gene amplification in Drosophila ovaries provides a means of increasing the amount of template for transcription, thus increasing the amount of protein that can be made over a short developmental period. At a specific developmental point (egg chamber stage l0B-13), several clusters …
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Flow-induced mechanotransduction in cell-cell junctions of endothelial cells
… an unexpected behavior shortly after the onset of laminar flow: their crawling speed decreases ~40% within the first 30 min, but only in a confluent monolayer of endothelial cells, not in subconfluent cultures, where cell-cell interactions are limited. This led us to study early shear effects on …
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Role of the ubiquitin-proteasome pathway in the inner ear : identification of an E3 ubiquitin ligase for Atoh1
… factor, is critical for the differentiation of inner ear hair cells. Hair cells do not develop in mice that lack Atoh1, and overexpression of the transcription factor in embryonic ears induces differentiation of extra hair cells. The level of Atoh1 expression is under the control of a Wnt and …
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Composite gelatin delivery system for bone regeneration
… properties and biocompatibility of gelatin were investigated to produce a gelatin scaffold for the release of bone morphogenetic proteins (BMPs) from composite particles. This delivery system, designed to regenerate bone, holds much promise as an alternative to bone grafts. The …
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Versuch einer Standardisierung der Exhalatkondensation
… war herauszufinden, ob sich das Volumen oder die Proteinmenge des Atemkondendats durch die Inhalation von Kochsalz (3 %) steigern läßt und ob sich die gemessenen Werte von Rauchern und Nichtrauchern unterscheiden. Es wurden Kondensatproben von 30 gesunden Probanden über verschiedene Zeiträume (5 …
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Development of immobilization and drying methods of enzymes on support particles for enzymatic gas-phase reactions
… by physical adsorption method. The effects of various immobilization and drying process parameters on the residual activity and the protein loading of the immobilized enzyme preparation were studied and thereby different optimum preparations were observed for different enzymes. Under the …
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Control of covalent and non-covalent presentation of biomolecules within collagen GAG scaffolds
… to be a major bottleneck in the development of biomaterials. The diffusive limitation of biomaterials restricts cell viability to regions where nutrients are readily available. To overcome this transport limitation, angiogenic biomaterials that utilize biomolecular cues such as Vascular …
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Chemical composition and physical properties of 20% milk fat reformulated creams manufactured from reduced cholesterol butteroil
… Transmission electron microscopic examination of reformulated cream emulsions showed an oil-in-water emulsion typical of milk lipid globules found in natural homogenized cream. Heat treatment (pasteurized and unpasteurized) and homogenization pressure (1500/500 PSI and 2000/500 PSI) had no …
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Spatial patterns of wheat grain composition in relation to nutritional quality and processing properties
The quality of wheat-based food. products, especially bread quality, is significantly affected by the characteristics of the wheat flour, both in term of processing quality and nutritional value. White flour, by far the most used flour in breadmaking, derives from grinding of the grain starchy …
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