Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"amnion"”.
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The Use of Amnion in Equine Wound Healing
… safety and efficacy of lyophilized milled human amnion as a wound dressing of experimentally created equine distal limb wounds. Animals: Four clinically normal adult horses (3 Thoroughbred and 1 Paint, median age 11 years) obtained via donation. Procedures: One forelimb of each horse was randomly …
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Regulation of amnion induction during the human formative pluripotency transition
… naïve hPSC into a formative like phase in vitro. Amnion is an extraembryonic membrane that supports the embryo by providing protection, nutrient exchange, and signalling cues. Unlike rodent models, in humans, the amnion segregates from the epiblast shortly after implantation; however, this process …
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Functional role of GSK3β in p38MAPK mediated human amnion membrane senescence
… with cell cycle arrest and senescence in amnion cells. Inhibitors to GSK3β and p38MAPK verified their roles. GSK3β inactivation was associated with nuclear translocation of antioxidant nuclear factor erythroid 2–related factor 2 (Nrf2) and exosomal section of β-catenin. OS-induced …
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The chick embryo amnion as an in vitro culture system for IVF and NT embryos
… quality of culturing IVP bovine embryos in the amnion of a domestic chicken egg was tested. In Experiment I, by culturing IVP bovine embryos in the chick amnion (day 4 to 7 of incubation) it was discovered that there was no significant difference in blastocyst rates compared with controls. In …
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mRNA-Expression des CRH-Systems in menschlichen schwangerschaftsspezifischen Geweben
… mit Myometrium, Plazenta, Dezidua, Chorion und Amnion von ca. 100 Patientinnen aufgebaut. <br>Zunächst wurden die RT-PCR-Methoden für alle o.g. Komponenten des CRH-Systems etabliert. Untersuchte Einflussgrößen waren dabei die RNA-Qualität, die Primerauswahl und die Austestung geeigneter …
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Pluripotency and patterning in nonhuman primate embryogenesis
… platform for lineage entry into EmDisc or amnion fate. Primed PSCs gave rise to amnion spheroids, in contrast to naïve PSCs. Pathway modulation revealed that Activin/Nodal and FGF signalling blocked amniogenesis, while BMP promoted amnion lineage entry. Investigating the combinatorial roles …
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Maintenance of fetal membrane integrity by cyclic cellular transitions during gestation and parturition
… However, the roles of membrane cells, amnion epithelial (AEC) and mesenchymal (AMC), and their ability to migrate, insert themselves, and repair these damaged sites (e.g., microfractures) remains unclear. We report that injury to AECs forces proliferation and cellular transitions …
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Feto-Maternal Exosome Characterization, Trafficking and Function During Pregnancy and Parturition
… reproduced this model in vitro to show human amnion epithelial cells can produce exosomes and when cultured under oxidative stress conditions, exosomes cargo signals of cellular damage and inflammation. Amnion cell-derived exosomes can traffic to the maternal tissues and produce …
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Intracellular signalling by arachidonic acid metabolites
… a non-enzymatic metabolite of PGD2, on amnion-derived WISH and JEG3 choriocarcinoma cells as models of the amnion and chorion trophoblasts, respectively.15d-PGJ2 induced apoptosis in both cell lines in a concentration-dependent fashion (2.5-10 µM). Apoptosis was characterised by …
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Spatial Transcriptome Profiling of Gastrulating Primate Embryos
… of the postimplantation embryonic disc and amnion, an extraembryonic membrane derived from the embryonic disc, and I tested the effects of NODAL, FGF, WNT and BMP on the in vitro models. Taken together, I found that FGF, together with NODAL, maintains pluripotency in the embryonic disc, …
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Chondrodystrophia foetalis in the foetus and the infant
… influences, most likely pressure of the amnion, to cause the deformities of achondroplasia. 6. The knowledge we possess of the metabolic influence of the pituitary and sexual glands points to an abnormal function of either or both of these structures as the most likely agent to cause the …
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Genesis of the human germline
… embryo, including mesoderm, endoderm, PGCs and amnion. I found a high degree of fidelity between in vitro PGCLC and bona fide early embryonic PGCs, which map spatially to a transition region in primate embryos in proximity to the amnion. I further identified TFAP2A as a critical early regulator …
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Characterization of Host Factors and Anti-viral Compounds for Diverse Mosquito-borne Flaviviruses
… neural stem cell lines, as well as primary human amnion cells. Established anti-flaviviral drugs (e.g., bortezomib and mycophenolic acid) and others that had no previously known antiviral activity (e.g., daptomycin) were identified as inhibitors of ZIKV infection. Several drugs reduced ZIKV …
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Regulation of Matrix Metalloproteinases and Their Inhibitors by DNA Methylation in Human Gestational Tissues
… and found to be increased in placenta and amnion at specific time points and treatments, correlating with mRNA expression levels. Interrogation of the TIMP-1 and MT1-MMP promoters using Sequenom™ EpiTyper® MassARRAY revealed general hypomethylation of the two genes, with sex-specific …
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The Role of Histone Deacetylases in Cytokine Regulation in Human Gestational Tissues
… expression was characterised in term placental, amnion and choriodecidual tissues obtained after elective caesarean section. All eighteen human HDACs were expressed, with tissue-specific differences in expression and production identified. A time course was then established to examine the …
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AMNIOTIC MEMBRANE TRANSPLANTATION FOR OCULAR SURFACE RECONSTRUCTION IN VETERINARY MEDICINE
… is composed by the chorion, allantois and amnion. The AM is the inner layer. It consists of a single layer of ectodermally derived cuboidal to columnar cells, which form the epithelium, a basement membrane that connects with the chorion by a layer of mesenchyme which contains large amounts …
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Illuminating the Black Box: Defining and Modeling Peri-Implantation Human Embryogenesis
… inducible human embryoids robustly generate amnion, extraembryonic mesenchyme, and primordial germ cell-like cells in response to BMP signaling. Using the modularity of this model, I identified an inhibitory role for SOX17 in anterior hypoblast specification. Modulation of the subpopulations …
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Eomes Directs the Formation of Spatially and Functionally Diverse Extra-Embryonic Mesoderm Derived Tissues
… the development of the embryo, including the amnion, chorion, yolk-sac, and allantois. Together, they protect the embryo and play key roles in the supply of nutrients and oxygen, waste management, haematopoiesis, and establishing the maternal-foetal interface. Embryonic stem cell (ESC)-derived …
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Finding Common Ground: The Common Marmoset as a Model to Accessing and Providing Insight into the Social Brain
… tube closure. During this protracted period, the amnion undergoes massive restructuring and growth, creating a unique opportunity to utilize this window to introduce genetic manipulations creating a proxy of a transgenic animal without conventional transgenic methodologies. This may be …