Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 259 for “"amino acid residues"”.
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Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing
… mutants in the C-terminal forty-one amino acid residues of LuxR, a region that has been hypothesized to play a critical role in the activation process. An <I>in vivo</I> luminescence assay was first used to test the effects of the mutations on LuxR-dependent activation of the …
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Amino Acid Residues Implicated in the Interaction of Melanocortin Ligands and Their Receptors: A Study of MC2R Selectivity
… variants of ACTH(1-24). Our results indicate an amino acid requirement in the C-terminal portion of ACTH(1-24) for activation, which supports the 2-step method of activation hypothesized for MC2R. This site, the tetra basic cleavage site, when altered does not stimulate cAMP production and does …
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Comparative analysis of cytotoxic necrotizing factor (CNF) toxins: Compatibility of cargo with delivery vehicle and identification of amino acid residues that modulate pH-dependent cytosolic cargo delivery
… sensitivity of the CNF toxins to endosomal acidification impacts their cargo delivery efficiency. We found that replacing particular acidic amino acid residues from the putative insertion-trigger motif of the CNFy translocation domain with those in CNF3 promotes endosomal escape at a higher …
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Development of tools for single molecule peptide fluorosequencing
… that combines selective fluorophore labeling of amino acid residues, total-internal reflection fluorescence (TIRF) microscopy, and Edman degradation, is a novel technology that has been developed to sequence peptides. Unique fluorophores are covalently attached to amino acid residues and through …
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Molecular Modulation of a-Subunit VISIT-DG Sequence Residue Asp-350 in the Catalytic sites of <em>Escherichia coli</em> ATP Synthase.
… the mechanism of ATP catalysis, critical amino acid residues involved in Pi binding have to be identified. The αVISIT-DG sequence at the interface of α/β subunits that contains residues from 345-351 is highly conserved and αAsp-350 has been chosen because of its negative charge side chain …
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The Evaluation of Conserved Amino Acids that Influence Polysubstrate Specificity in the Multidrug Resistance Transporter, EmrE
… mutagenesis analysis to alter conserved amino acid residues within the Escherichia coli small multidrug transporter, EmrE. The EmrE variants generated were further assessed for their resistance ability to 19 structurally different quaternary cationic compounds (QCC) using a …
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C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication
… HBV DNA replication, however critical motif or amino acid residues in the RNase H domain for the HBV replication has not been extrensively demonstrated yet. In the present study, several chimeras of P protein by substituting Duck hepatitis B virus (DHBV) sequences were constructed. Accordingly, …
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A Transmembrane Precursor of Secretory Component
… corresponding sequence of the COOH-terminal 163 amino acid residues of the SC precursor. The SC precursor has a putative membrane spanning segment of 23 non-charged amino acid residues, followed by a cytoplasmic tail of 103 amino acid residues with a preponderance of charged and hydrophilic …
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The contribution of His7.36(305) of the GnRH receptor to ligand binding and receptor activation
… to give insights into the role of individual amino acid residues of both the GnRH receptor and the GnRH ligand in receptor function. A His7.36(3o5) residue on transmembrane helix seven of the GnRH receptor was investigated for its contribution to the overall function of the receptor.
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The localization of two epitopes recognized by the monoclonal antibody PCG-4 on toxin A of Clostridium difficile
… a 2.5 kb region, containing 38 similar repeating amino acid sequences, at the 3' -end of the gene. This region of the toxin A gene codes for the carbohydrate-binding portion of the toxin. The monoclonal antibody PCG-4 (MAb) binds to this portion of toxin A and neutralizes its enterotoxic activity. …
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The cloning of novel gonadotropin-releasing hormone receptors by polymerase chain reaction
… role of the pituitary GnRHR. Elucidation of amino acid residues of the GnRHR that are crucial for ligand binding, activation of the receptor, and coupling to the G-protein, is important in understanding structure-function relationships towards the design of drugs for therapeutic intervention. …
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Structure and function of V1b vasopressin receptor
… crystallography. This study aimed to identify amino acid residues which participate in ligand binding of the V1bR by site-directed mutagenesis with the aid of molecular models of vasopressin receptors based on the crystal structure of bRho. The V1bR is a potential drug target in treating …
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Novel Approaches for the Measurement of Unusual Post-Translational Modifications in Neuropeptides
Other PTMs, such as the transformation of L-amino acid residues to the D-configuration, do not result in a measurable mass change. Capillary electrophoresis combined with mass spectrometry is used to identify cells containing a D-amino acid-containing peptide found in Aplysia. These cells are then …
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Active Insulin Analogs That Do Not Require Refrigeration
… to hydrophobic interactions between nonpolar amino acid residues, molecules of insulin tend to interact with each other and undergo fibrillation to form linear aggregates when partially unfolded. Formation of these insulin fibrils significantly decreases insulin stability and activity, and has …
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Investigating Cytochromes in Photosynthetic Electron Transport in Cyanobacteria
… The aim of this study was to identify the amino acid residues in cytochrome b6 that participate in plastoquinol and inhibitor binding at the plastoquinol oxidation site of the cytochrome bf complex. This study served as a basis for developing a structural model of binding sites for several …
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Recombinant Expression of Preptin Analogs for Alanine Scanning Mutagenesis of Residues 27-29
<p>The novel peptide preptin consists of 34 amino acid residues corresponding to Asp<sup>69</sup>-Leu<sup>102</sup> of proinsulin-like growth factor E-peptide. Preptin has been isolated from pancreatic beta cells, and it has been found to exhibit both metabolic and mitogenic activities. This makes …
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Elucidation of antigenic epitopes on the rubella virus E1 glycoprotein
… synthetic peptides. HA epitope was mapped to amino acid residues E1214 to E12„ while two VN epitopes mapped to amino acid residues E1214 to E12, and E1219 to E1232. The potential use of the defined epitopes in the development of subunit vaccine is discussed.
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The Rational Design, Synthesis, And Biological Evaluation Of Small Molecule Cd4 Mimetic Families For Hiv-1 Viral Entry Inhibition
… interactions with highly conserved amino acid residues that lead CD4 mimetic BNM-III-170 engages with in the gp120 binding cavity but also by branching into new chemical space to establish hydrogen-bonding interactions with other additional, conserved amino acid residues. With the …
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Biochemische und molekularbiologische Charakterisierung von Spike, einem neuen BH3-Domänen Protein
… that codes for a novel BH3-only protein of 219 amino acid residues which was named Spike, for Small protein with inherent killing effect. Spike was then in the course of my PhD thesis extensively characterised, molecularly and biochemically. In summary, upon overexpression in mammalian cells …
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Expression, sequencing, and characterization of mannitol-1- phosphate dehydrogenase genes from Aspergillus parasiticus and Escherichia coli
… homologous and the identity was 88.7% at the amino acid level and 81.6% at the nucleotide level. The two genes translate into polypeptides of equal numbers (382) of amino acids with M, of 40,880 and 41,221, respectively. The possible NAD binding sites were identified for both enzymes in the …
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