Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 5 of 5 for “"amino acid radical"”.
-
Photolyase: Its Damaged DNA Substrate and Amino Acid Radical Formation During Photorepair
… and damaged DNA permitted examination of nucleic acid changes upon formation of the CPD lesion; these experiments indicated that C=O and C-H frequencies can be used as markers for DNA damage. Furthermore, in purified photolyase containing isotopically-labeled aromatic amino acids, we observed that …
-
Photochemical ribonucleotide reductase for the study of proton-coupled electron transfer
… transport and catalysis in enzymes often rely on amino acid radicals as intermediates. The generation and transport of these radicals are synonymous with proton-coupled electron transfer (PCET), which intrinsically is a quantum mechanical effect as both the electron and proton tunnel. The caveat …
-
Mechanistic investigations of the radical initiation pathway of class I ribonucleotide reductase from Escherichia coli
… rate. R2 contains the diferric-tyrosyl (Y.) radical cofactor that initiates nucleoside reduction by a putative long-range proton-coupled electron transfer (PCET) pathway over 35 A. This pathway is thought to involve specific amino acid radical intermediates (Y22-> W48-> Y356 within R2 to …
-
Dioxygen activation and substrate hydroxylation by the hydroxylase component of toluene/O-xylene monooxygenase from pseudomonas sporium OX1
… cluster spin-coupled to a neutral W radical. ENDOR spectra of this intermediate confirmed the protonation state and type of the amino acid radical and also identified a labile terminal water or hydroxide on the diiron center.
-
Proton-coupled electron transfer in the Escherichia coli ribonucleotide reductase
… their corresponding deoxynucleotides via a thiyl radical hydrogen abstraction mechanism. As this is the only cellular pathway for production of the monomeric precursors required for DNA synthesis, the regulation of RNR is crucial to controlling the cell cycle by the availability of substrate pools …