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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"amino acid oxidase"”.
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The Oxidative Deamination of Sulfur Amino Acids by a Bacterial L-Amino Acid Oxidase
<p>The oxidative deamination of sulfur amino acids by a particulate L-amino acid oxidase obtained from an atypical Proteus rettgeri has been investigated. In addition to several sulfur amino acids tested here the enzyme catalyzes the oxidation of L-leucine, L-phenylalanine, L-tryptophan, …
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CYTOTOXICITY MEDIATED BY L-AMINO ACID OXIDASE ISOLATED FROM THE VENOM OF CROTALUS MITCHELLII PYRRHUS
… examines the mechanism of cytotoxicity of an L-amino acid oxidase isolated from snake venom in prostate cancer cell line. The enzyme causes oxidative stress, induces ER stress and activates autophagy in the cells in vitro, leading to the activation of apoptosis.
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Methodological and biological studies of the amino acid oxidases.
… in which the liberation of ammonia from amino acid substrates by the D- and L-amino acid oxidases is coupled to the NALH dependent reductive amination of 2-oxoglutarate, the reaction being catalysed by exogenous glutamate dehydrogenase L-glutamate: NAL(P) oxidoreductase (deaminating), EC …
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Phylogenetic Analysis Suggests Horizontal Gene Transfer Event Within the Mitis Group Within the Genus Streptococcus Involving L-Amino Oxidase In Streptococcus Oligofermentans
… previously resolved in vitro and isolated to L-amino acid oxidase (LAAO) within S. oligofermentans which produces high levels of H2O2 through the metabolism of seven L-amino. Phylogenetic analysis of coded amino acid (AA) sequences within 20 enterococci present insights into possible methods for …
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The Design, Development and Characterisation of a new Biosensor for In-vivo Neurochemical Monitoring of ᴅ-Serine
… biosensor based around the flavin enzyme ᴅ-amino acid oxidase. In the recent past ᴅ-serine has been elevated from an “unnatural” amino acid to be recognised as a very important neurotransmitter that could be responsible for the regulation of a large portion of glutamate signalling in the …
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Discovery of high quality molecular leads and screening collections for CNS drug targets
… allowed the de novo design of a novel class of D-amino acid oxidase (DAAO) inhibitors. This work gave rise to three novel series of DAAO inhibitors, all of which were capable of addressing some or all of the issues that had limited previous attempts to inhibit this high profile CNS target. …
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Applications of Engineered Proteins in Redox Biology and Biomarker Detection Assay Development
… encoded hydrogen peroxide generator, D-amino acid oxidase (DAAO), was used to understand the hours-long intracellular hydrogen peroxide (H₂O₂) generation. This study elucidated that the primary respondent of cytosolic H₂O₂ is peroxiredoxin 1 and the H₂O₂ induced apoptosis initiates …
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Development of an Ionically-Assembled On-Column Enzyme Reactor for Capillary Electrophoresis
… mixture of the negatively charged enzyme glucose oxidase (GOx) and anionic poly(styrenesulfonate) (PSS). The reaction of glucose with the immobilized glucose oxidase produces H2O2 which migrates the length of the capillary under the influence of electroosmotic flow and is detected amperometrically …
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Enantioseparation using a counter-current bioreactor
… using as a model reaction the isolation of L-amino butyric acid (L-ABA) from a DL-ABA racemate and the enantioselectivity of D-amino acid oxidase (DAAO). Bioconversion of D-ABA to ketobutyric acid (KBA) by DAAO, immobilised by selective partitioning in the stationary phase of the CCC …
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Analysis of endogenous D-amino acids in the central nervous system and endocrine system via capillary electrophoresis with biochemical sample treatment
… Due to their unique chirality, endogenous D-amino acids are considered to play different roles from their corresponding L-isomers. In the central nervous system, a few D-amino acids have been found and they have increasingly gained research interest because their localization suggests …
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The role of Interleukin-4 induced gene 1 (IL-4i1) in allergic asthma and atopic dermatitis
… induced gene 1 (IL-4i1) is a secreted L-amino acid oxidase enzyme mainly expressed by antigen-presenting cells (APCs) and upon activation by IL-4 and CD40, can be induced in B lymphocytes. IL-4i1 converts phenylalanine into phenylpyruvate, ammonia, and hydrogen peroxide which can induce …
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Structural and Biochemical Studies of Streptococcus sp. alpha-Glycerophosphate Oxidase
… kinase (GlpK), and either alpha-glycerophosphate oxidase (GlpO) or dehydrogenase (GlpD). GlpF aids in bringing glycerol into the cell, while GlpK phosphorylates it, trapping it within the cell as alpha-glycerophosphate (Glp). Glp is converted to dihydroxyacetone phosphate (DHAP) by GlpO in certain …
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Towards next-generation enzymatic generators of hydrogen peroxide in quantitative redox biology
… adenine dinucleotide phosphate (NADPH) oxidase. However, these methodologies add conflating variables of extracellular H₂O₂, a gradient between extracellular and intracellular species, and production of superoxide (02-) as an intermediate, complicating interpretation of resulting …
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Elektroenzymatische Synthese mit ionischen Flüssigkeiten
The electrochemical generation, respectively regeneration of redox equivalents represents an interesting alternative to the for this purpose usually applied methods. However, this method has some drawbacks, which make their application as industrial processes economically unfeasible. Especially, …