Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"alternative polyadenylation"”.
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Alternative Polyadenylation Modulates Expression of Pro-Fibrotic Proteins and Contributes to Lung Fibrosis
… factor 25, CFIm25) is a key regulator of alternative polyadenylation (APA). NUDT21 depletion causes 3’UTR shortening via APA leading to enhanced mRNA stability and protein translation. This NUDT21 reduction promotes tumor growth in glioblastoma by enhancing expression of oncogenes. Cancer …
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Role of Nudt21 Mediated Alternative Polyadenylation and Hyaluronan In The Development of Pulmonary Hypertension
… exist for this disease. It has been shown that alternative polyadenylation (APA) due to depletion of Nudix Hydrolase 21(NUDT21) is involved in several disease states including the chronic lung disease idiopathic pulmonary fibrosis (IPF). Additionally, hyaluronan, an extracellular matrix …
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The Role of Alternative Splicing and Alternative Polyadenylation in Regulating Activated T cell Biology
Alternative splicing and alternative polyadenylation are now recognized to occur in the vast majority of human genes. Both have the potential to alter the identity of the encoded protein, as well as control protein abundance, protein localization, or association with other factors. T cells provide …
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Twist of messenger Fate: novel mechanisms for TDP43 in modulating mRNA decay and alternative polyadenylation
… striking role in the 3’UTR, modulating mRNA alternative polyadenylation and promoting the generation of shorter transcripts. This finding is supported by the direct interaction of TDP43 with the cleavage stimulation factor, a core component of the polyadenylation machinery. These results …
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Prediction of mRNA polyadenylation sites in the human genome and Mathematical modeling of alternative polyadenylation
Messenger RNA (mRNA) polyadenylation plays many important roles in the cell, such as transcription termination, mRNA stability and transportation, and mRNA translation in eukaryotic cells. A large number of human and mouse genes have multiple polyadenylation sites (referred to as poly(A) sites) …
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Regulation of p21 Function by a Sense Long Non-Coding RNA Generated by Alternative Polyadenylation
… method widely used to control gene expression is alternative polyadenylation (APA). Approximately 70% of eukaryotic transcripts have multiple possible polyadenylation sites (PAS), allowing expression of mRNA isoforms with different 3’ untranslated regions (3’ UTR) as well as different open reading …
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Posttranscriptional regulation with regard to cleavage factor I (CFI) associated alternative polyadenylation and methyltransferase A (MTA) assisted m6A modifications in Arabidopsis thaliana
… which are affecting the fate of a transcript. Alternative polyadenylation (APA) is associated with many regulatory aspects. It describes the addition of the poly(A) tail at alternative sites inside the transcript. This is achieved by the cleavage and polyadenylation machinery. Cleavage factor I …
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Deconvoluting Cell-Type Specific 3'UTR Isoform Expression in the Adult and Developing Cerebellum
<p>Alternative polyadenylation has been implicated in the regulation of mRNA translation and stability, as well as mRNA and protein localization. However, it is unclear to what extent alternative polyadenylation regulates these processes uniquely in specific cell types. Using a newly developed …
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Bioinformatics study of mammalian MRNA polydenylation
Messenger RNA polyadenylation is one of the key post-transcriptional events in mammalian cells, which have influences on many aspects of mRNA metabolism. Several human diseases have been shown to associate with abnormal polyadenylation, highlighting the importance of this process. The availability …
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The Role of Cleavage and Polyadenylation Spcific Factor 6 (CPSF6) in Hematopoiesis
<p>Cleavage and polyadenylation specific factor 6 (<em>Cpsf6</em>) translocation is frequently reported in APLL, which is Acute Promyelocytic Leukemia (APL)-like acute myeloid leukemia (AML) and other types of blood diseases associated with abnormal hematopoiesis. CPSF6 is a subunit of the Cleavage …
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High-throughput sequencing of RNA 5'- and 3'-termini yields insights into viral and vertebrate gene expression
… at the 5' ends of influenza transcripts and alternative polyadenylation at the 3' ends of vertebrate transcripts. The 5' ends of influenza mRNAs include heterogeneous sequences derived from host RNAs. In a process termed cap snatching, the viral polymerase cleaves host RNAs ~10-13 nucleotides …
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Polyaseeker: a computational framework for identifying polyadenylation cleavage site from RNA-seq
Alternative polyadenylation (APA) of mRNA plays a crucial role for post-transcriptional gene regulation. Recently, advances in next generation sequencing technology have made it possible to efficiently characterize the transcriptome and identify the 3'end of polyadenylated RNAs. However, no …
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Regulation and Function of the Dscam1 Extended 3' UTR mRNA
… in species from Drosophila to human undergo alternative polyadenylation (APA). It has been shown that neuronal tissues are highly enriched for usage of transcripts with extended alternative 3' untranslated regions, which are products of APA. Specific function of these longer 3' UTR …
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Spatiotemporal modelling in biology: from transcriptional regulation to plasmid positioning
… chromatin silencing mechanism, where alternative polyadenylation of antisense transcripts is linked to changed histone modifications at the locus and altered expression. Mathematical model predictions of FLC transcriptional dynamics are validated by measurements of total and …
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Alternative isoform regulation in myotonic dystrophy
… that essentially 92-94% of all human genes are alternatively spliced, 86% of them with a minor isoform frequency 15% or more. We found that the majority of alternative splicing and alternative polyadenylation and cleavage events are tissue-regulated, and that patterns of these RNA processing …
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The structure and sequence of the single calmodulin gene of Drosophila melanogaster
… sequencing, were found to result from the use of alternative polyadenylation signals. Northern blot analysis of polyadenylated transcripts isolated from 0 to 12 hour-old embryos confirmed the existence of two mRNAs of the approximate sizes 1.7 and 1.9 kilobases. Northern blot analysis of poly-A+ …
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Integrative analysis of transcriptomic data to elucidate regulators of pre-mRNA processing
Alternative pre-mRNA processing events, including alternative splicing (AS) and alternative polyadenylation (APA), are key drivers of transcriptomic and proteomic diversity. This dissertation presents a comprehensive framework for analyzing these events, emphasizing improvements to computational …
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Computational methods for the analysis of next generation sequencing data
… a novel bioinformatics pipeline for identifying polyadenylation cleavage sites from RNA sequencing data, which helps to enhance the knowledge of alternative polyadenylation mechanisms and their roles in gene regulation. A change-point model based on a likelihood ratio test is also proposed to …
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Quantification of microRNA regulation and its consequences at the single cell level
… that the reporter systems could be used to study alternative polyadenylation, which is usually accompanied by consequential loss of MREs.
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