Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"alpha-smooth muscle actin"”.
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Expression of alpha-smooth muscle actin and contraction of collagen-glycosaminoglycan scaffolds by cells derived from canine synovium
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2003.
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Determining alpha-smooth muscle actin expression in embryonic and mesenchymal stem cells of assorted mammals seeded in collagen scaffolds in vitro
… by regeneration but the mechanism is unknown. Alpha-smooth muscle actin ([alpha]-SMA) is the protein responsible for contraction, thus determining if it is present in embryos which heal by regeneration will further our knowledge about the causes of regenerative healing. This thesis …
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Microfluidic platforms to evaluate the effects of drugs on fertility and proteins on cancer progression – a focus on cisplatin, alpha-smooth muscle actin, and glial cell line-derived neurotrophic factor
The introduction of in vitro systems that can mimic organ physiology and allow studying the progression of diseases such as cancer is necessary to get reliable information about cell responses and test drug efficacy and toxicity. Classical cell culture systems facilitate a wide range of clinical in …
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Mechanical Deformation of the Extracellular Matrix Mediates Cellular Activation and Mechanotransduction
… &beta 1 integrin, and the contractile protein, &alpha-smooth muscle actin (&alpha-SMA) . We report that mechanical loading induces RBL-2H3 degranulation via RGD integrins and regulates age-dependent differences in collagen I and III gene expression in neonatal and adult fibroblasts. We also …
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Control of flow and oxygen in a 3-D perfused micro-environment fosters balanced survival of hepatocyte-non-parenchymal cell co-cultures
… associated with activation of stellate cells (alpha-smooth muscle actin staining).
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Characterization and modeling of cell activation upon TGF-β1 stimulation in a 3-D culture system
… and expression of a marker protein, alpha-smooth muscle actin (α-SMA). The cell response with TGF-β1 stimulation was captured with immunofluorescence staining (IF) and confocal microscopy imaging of α-SMA in each cell embedded layer. Our results revealed the spatial-temporal profiles …
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In vitro pharmacological inhibition of myofibroblast differentiation and force generation in a collagen-GAG matrix
… and expression of the contractile filament [alpha]-smooth muscle actin ([alpha]-SMA), a hallmark of the myofibroblast phenotype. NR6WT fibroblasts were seeded into free-floating cylindrical CG matrices (8 mm in diameter, n=6) and treated with serum-containing media supplemented with …
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The recruitment of stromal cells to the site of tumor formation
Myofibroblasts are an alpha-smooth muscle actin ([alpha]-SMA)-expressing cell type found within human mammary carcinomas, but not in the normal mammary gland. Myofibroblasts can enhance tumor formation by promoting angiogenesis and invasion, and we therefore sought to better understand how …
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Inhibition of myofibroblast contraction
… thus improve the quality of nerve regeneration. Alpha smooth muscle actin ([alpha]-SMA) is a critical contractile protein. Its expression can be upregulated by the growth factor TGF-[beta]1, and blocked by the pharmacological agent PP2. To investigate whether blocking SMA expression alone can …
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The role of microvesicles in EMT and tumour microenvironment
… high level of myofibroblasts marker alpha-smooth muscle actin (α-SMA) cytoskeleton protein and FGF. MVs were isolated from the myofibroblasts were enriched with α-SMA protein. Primary fibroblasts were treated with MVs released myofibroblasts expressed high level of α-MSA protein. …
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Proteomic comparison of biomaterial implants for regeneration of peripheral nerve tissue
… 1, 2, 3 (TGF [beta]1, [beta]2, and [beta]3) and alpha smooth muscle actin ([aipha] SMA) to measure disparities in proteins associated with wound healing that may determine nonregenerative from regenerative outcomes. Transected nerves treated with silicone or collagen tubes were compared on a …
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Host-intrinsic factors determine anti-tumor efficacy of exercise in pancreatic ductal adenocarcinoma
… observed a gut microbiome dependent reduction in alpha smooth muscle actin (αSMA<sup>+</sup>) cells and <em>Il6</em> expressing inflammatory cancer associated fibroblasts (iCAFs). Similar analysis of tumors from patients in the PancFit trial demonstrated an exercise induced reduction in …
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Modulation of TGFβ1-induced Fibroblast-to-Myofibroblast Transition in response to Prostaglandin E2 Production by Human Rhinovirus-Infected Airway Epithelial Cells
… asthmatics and is known to upregulate fibroblast alpha-smooth muscle actin (α-SMA) expression, a characteristic marker of myofibroblasts. Given that human rhinovirus (HRV) infections are postulated to be involved in the pathogenesis of airway remodeling in asthma, we originally hypothesized that …
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Vascular smooth muscle cell heterogeneity and plasticity in models of cardiovascular disease
Vascular smooth muscle cell (VSMC) accumulation is a hallmark of atherosclerosis and vascular injury. However, fundamental aspects of proliferation and the phenotypic changes within individual VSMCs, which underlie vascular disease remain unresolved. In particular, it is not known if all VSMCs …
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The Role of The Hypoxia-Inducible Factor 2 In Pancreatic Cancer: Mechanisms of Tumor Immunosuppression and Intestinal Radioprotection
… contributes to its therapeutic recalcitrance. Alpha-smooth muscle actin+ (αSMA+) cancer-associated fibroblasts (CAFs) are the most abundant stromal component, as well as mediators of stromal deposition. The hypoxia-inducible factors (HIF1 and HIF2) coordinate responses to hypoxia, yet, despite …
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Synovial Fibrosis in Osteoarthritis of the Knee: Mechanism of Development and Potential Therapeutic Targets
… to a myofibroblast phenotype characterized by alpha smooth muscle actin (a-SMA) upregulation and stress fiber formation. Transforming growth factor-β (TGF-b) plays an important role in mediating the pathogenesis of synovial fibrosis. Synoviocytes produce proteoglycan-4 (PRG4; lubricin), a …
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Tumor-Cell Derived Inhibin, Beta A Is Essential For Adrenergic Effects On Fibroblast Activation
… or non-stressed controls were assessed for alpha-smooth muscle actin (a-SMA) expression. Normal fibroblasts (NoF 151) were treated with conditioned media from non-treated and norepinephrine (NE) treated Skov3 and HeyA8 cells and analyzed for induction of CAF-phenotype by a-SMA expression. …
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The Role of Transforming Growth Factor-Beta In Alcohol-Induced Cardiac Fibrosis
… growth factor beta (TGF-β), and the presence of alpha smooth muscle actin (α-SMA)-positive cells were determined. A compensatory period in mice treated with ethanol occurred early followed by a transition to a dilated phenotype over time. A number of factors may be involved in this process …
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Enteric and hepatic glia-like cell reactivation after astrocyte-directed twinkle helicase loss
… be identified. In addition, another HSC marker, alpha smooth muscle actin, or the tdTomato protein did not bring any further results. We did not analyze mtDNA amount in situ, which would be a consequence of Twinkle loss, provided that this helicase acts as the replicative helicase in the …
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Corneal fibrosis therapies and investigations in the canine corna in vitro
… in expression of profibrotic markers, including [alpha]-smooth muscle actin ([alpha]-SMA; p<0.0001), Fibronectin (FN; p = 0.0002), Tenascin C (TNC; p<0.0001), Collagen I (Col-I; p<0.0001), Collagen IIIA1 (Co-IIIA1; p<0.0001), and Collagen IV (Co-lV; p<0.0001). Conclusion: An ophthalmic formulation …
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