Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"alpha-ketoglutarate"”.
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A Cell-Based Model to Study Factors that Drive Diffuse Astrocytoma Development
… D-2-hydroxyglutarate (2-HG) instead of alpha-ketoglutarate (α-KG). As 2-HG accumulates, it induces a hypermethylator phenotype that prevents the cells from differentiating. Acquisition of additional mutations in tumor suppressors such as p53 and/or ATRX likely leads to tumor initiation. …
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Two versatile cofactors, flavin adenine dinucleotide and non-heme iron, involved in DNA repair and natural product halogenation
… of alkylating agents, is a member of the alpha-ketoglutarate (aKG) non-heme iron-dependent superfamily. It uses the cofactor non-heme iron, and the co-substrates molecular oxygen a: G, to remove alkylated adducts on DNA bases via hydroxylation. Two well characterized 1 homologues of AlkB, …
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Study of PVCA and PVCB, two enzymes involved in making isonitrile-containing natural products in bacterial pathogens
… To demonstrate PvcB as an Fe[2+ superscript], [alpha]-ketoglutarate dependent oxygenase, and as a source of the diversity in isonitrile-containing metabolites, reactions catalyzed by the PvcB homologous proteins from Pseudomonas aeruginosa, Xenorhabdus nematophila and Erwinia amylovora were …
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The intersection of metabolism and oxygen in T cell function and anti-tumour immunity
… glutarate as a competitive inhibitor of alpha-ketoglutarate dependent dioxygenases (αKGDDs) and explores the effect of glutarate on hypoxia-inducible factor prolyl-hydroxylases (PHDs) and on DNA and histone methylation. Chapter V focuses on the role of glutarate in cellular metabolism and …
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Interrogating Cancer Metabolism using Novel Imaging Techniques
… transcriptional profiles through regulation of alpha-ketoglutarate metabolism. In vivo imaging of BCAT1 activity is therefore suggested as a potential technique for patient stratification and potentially treatment monitoring. [1-13C]Ketoisocaproic acid was successfully hyperpolarised and showed …
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The Role of Nutrient Availability in Therapeutic Response of Leukemia
… TPP is critical for the activity of alpha-ketoglutarate dehydrogenase (AKGDH), a TCA cycle enzyme that catalyzes a step in the overall conversion of glutamine to asparagine. When TPP availability is limiting for cell proliferation of TPK1 KOs, ASNase sensitivity is significantly …
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Microenvironmental and metabolic influences in tumor cell redox homeostasis
… metabolism to resist glutathione oxidation. Alpha-ketoglutarate, a TCA cycle carbon and downstream metabolite of glutamate, can prevent mitochondrial glutathione oxidation and aid GSH biosynthesis during glutamine deprivation. However, alpha-ketoglutarate cannot restore proliferation to …
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Molecular phenotypes associated with heifer fertility
… genes (APMAP and DNAI7), as well as one protein (alpha-ketoglutarate-dependent-dioxygenase FTO), that differed significantly between fertile and sub-fertile heifers. Importantly, the results of this study allowed us to create a biological profile that was capable of accurately distinguishing 21/22 …
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Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
… cycle, pentose phosphate pathway, pyruvate node, alpha-ketoglutarate node and oxaloacetate node changed. By comparing the metabolic flux maps of E. coli MG1655 grown at different conditions, pathways that have flux change under stress were identified. Inhibition effect for several pathways, …
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Investigations into the molecular basis of enzyme-catalyzed reactions in natural product biosynthesis
… biochemical characterization of an Fe(II)- and alpha-ketoglutarate dependent dioxygenase that is capable of degrading phenoxy and aryloxyphenoxy propanoate based herbicides. Coupled with structural analyses performed by a previous lab member, these studies reveal the molecular basis behind …
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The stimulation or inhibition of growth and acid precipitable material production of Vibrio Fetus by Kreb's cycle intermediates, fatty acids, amines, and miscellaneous compounds
… a medium which increased the growth of V. fetus. Alpha-ketoglutarate and tween 80 increased APM production of V. fetus, although these compounds neither increased nor decreased the growth of the vibrios. Therefore, the addition of α-ketoglutarate and tween 80 to the medium used for maximal …
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Analysing concerted criteria for local dynamic properties of metabolic systems
… instabilities were mutations in the enzyme alpha-ketoglutarate dehydrogenase.
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Lipoic acid metabolism in Leishmania major
… LA is also an essential cofactor of the alpha-ketoacid dehydrogenase complexes (alpha-KADHs) and of the glycine cleavage complex (GCC). The alpha-KADHs include the pyruvate dehydrogenase (PDH), branched-chain alpha-ketoacid dehydrogenase (BCKDH) and alpha-ketoglutarate dehydrogenase …
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Lipoic acid protein ligases in Plasmodium spp.
… 6,8-thioctic acid, is an essential cofactor of alpha-keto acid dehydrogenase complexes (KADH) and the glycine cleavage system (GCV). The KADH include the pyruvate dehydrogenase (PDH), branched chain alpha-keto acid dehydrogenase (BCDH) and alpha-ketoglutarate dehydrogenase (KGDH), which are an …
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Metabolic Coordination of Stem Cell Fate Controls Tumor Initiation and Tissue Repair
… which in turn produces the metabolite α-ketoglutarate (αKG). αKG stimulates terminal differentiation via activation of αKG-dependent dioxygenases that remove the repressive histone modification H3K27me3, which otherwise promotes EpdSC self-renewal. Accordingly, serine starvation or …