Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"allosteric activation"”.
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Allosteric activation of periplasmic HtrA proteases
… stabilize an inactive conformation of DegS. Allosteric activation occurs as a consequence of the linked binding of unassembled outer-membrane proteins to the PDZ domains and RseA to the DegS active sites. In Chapter 2, I probe the pathway of communication between the PDZ and protease domains …
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Regulation and role of the LKB1-AMPK pathway
… of Thr172, a conserved residue in the activation loop of the kinase domain, by either LKB1 or CaMKKß. Increases in Thr172 phosphorylation can be mediated by either protecting this site against dephosphorylation by protein phosphatases, or by promoting its phosphorylation by upstream …
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Studies of PARP-1 activation and inhibition using NMR spectroscopy
… automodified in response to DNA damage. PARP-1 activation occurs following recognition of single or double strand breaks by PARP-1’s N-terminal zinc finger domains. A multi-domain allosteric activation pathway leads to conformational changes in the HD subdomain of the catalytic domain that …
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Functional analysis of hepatitis C virus non-structural protein (NS) 3 protease and viral cofactor NS4A
… aim of this thesis was characterization of NS3 allosteric activation by its viral cofactor, NS4A. We hypothesized that there would be specific residues that dominate the interaction between NS3 and NS4A, and further hypothesized that binding and activation may be separate events mediated by …
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PLASTICITY IN PROTEIN SEQUENCE-FUNCTION RELATIONSHIPS
… occurrence and diverse cellular roles of allosteric proteins, designing novel allosteric proteins is of great interest for applications in synthetic biological and disease treatment. However, the engineering of allostery is often hindered by our limited understanding of the protein …
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Identification of A Conserved Cluster In The Rh Domain of Grk Critical For Activation By Gpcrs
… rhodopsin kinase and GRK2 suggests there is an allosteric docking site for the receptor distinct from the GRK catalytic site. While the agonist-activated GPCR appears crucial for GRK activation, the molecular details of this interaction remain unclear. Recent studies suggested an important role …
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Role of the RING domain in MDM2-mediated ubiquitination of p53
… hydrogen deuterium exchange that UbcH5α can be allosterically activated by MDM2. A novel peptide phage display technique linked to next generation sequencing was developed to further confirm an allosteric change and demonstrates that UbcH5α has different binding specificity for peptides when in …
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Modeling cellular metabolism in multiple scales
… the binding of ligands in active sites, and for allosteric activation of enzymes. In order to identify essential residues for communication pathways and signaling elements in biomolecular complexes, we developed an evolution of the dynamical network analysis framework. The new implementation was …
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Paraspeckle protein NONO regulates active chromatin by allosterically stimulating NSD1
… euchromatin integrity, and its loss or aberrant activation has been implicated in human congenital disorders, such as Weaver and Sotos syndromes, as well as broad types of cancers, including Diffuse Midline Glioma (DMG) and a subset of Acute Myeloid Leukemia (AML). This dissertation presents …
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Characterising the conformational dynamics of β₁-adrenergic receptor activation and G protein coupling
… changes in a highly interconnected allosteric activation network which enables coupling to intracellular signalling partners, including selective coupling to specific G protein α-subtypes. Investigations in solution by nuclear magnetic resonance spectroscopy (NMR) have been utilised …
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Using Small Molecule Tools to Study ATPase Mechanoenzymes
… and ADP-bound states, and reveal VA1 binding an allosteric pocket near the C-terminus in both states. Finally, I engineered mutations in the VA1 binding site that confer resistance to VA1, and furthermore, modulate VCP ATPase activity to a similar level as VA1-mediated activation. Together, these …
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Aspects of purine and pyrimidine metabolism
… and 2,3- bisphosphoglycerate (2,3-DPG). These allosteric effectors influence, in opposing ways, two enzymes governing IMP accumulation, namely PRPP synthetase and 5'-nucleotidase. These metabolic properties suggest that the erythrocyte could play a role in the removal of hypoxanthine from …