Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"allelic series"”.
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FLP-mediated conditional loss of an essential gene to facilitate complementation assays
… desirable to replace an essential gene with an allelic series of mutated genes, or genes with altered expression patterns, the complementing constructs are introduced into heterozygous plants, followed by the selection of homozygous null segregants. To overcome this laborious and time-consuming …
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Molecular Genetics and Subcellular Localization of Flavonoid Metabolism in Arabidopsis
… of mutant CHS genotypes were identified in this allelic series, including ones that displayed both null and temperature-sensitive phenotypes, based on endproduct analysis. Characterization of protein and RNA levels indicated that the stability of the CHS enzyme was reduced in some of the mutants …
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New Targets of p53 Regulation: From Aging to Immune Response
… dosage in an in vivo context. By developing an allelic series through multiple genetic crosses, I obtained mice with incrementally increasing levels of constitutively active p53. I describe how p53 dosage results in differential physiological outcomes with respect to lifespan, fertility, cell …
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GENETIC CONTROL OF EYE AND CENTRAL NERVOUS SYSTEM DEVELOPMENT
… genes in neural development, by generating an allelic series of knock-in models at the Pax6 locus. We showed that Pax6(5a) and Pax2 could not replace Pax6 for its auto-regulation in lens induction or for neural differentiation in retina. In brain development, however, we demonstrated that cell …
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Genetic dissection of carotenoid concentration and compositional traits in maize grain
… almost all carotenoid intermediates, revealed an allelic series associated with a large reduction in the major carotenoid intermediates of maize grain. This QTL, encoding carotenoid cleavage dioxygenase 1 (ccd1), showed a substrate preference for all measured carotenoids except beta-carotene. We …
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Functional Investigation of Genetic Determinants of Red Blood Cell Traits
… I assessed the consequences of mono- and biallelic GMPR disrupting mutations on cellular proliferation, erythroid differentiation and gene expression in iPS-derived erythroblasts. Complementing the experiments in cellular models, I designed and conducted a recall-by- genotype study in …