Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"allelic exchange"”.
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Analysis of the vlpA genes and their roles in the pathogenesis of Vibrio cholerae O139 / Pranom Kaewrakon.
… O139 which contains two copies of vlpA, by allelic exchange after inactivation via the insertion of kanamycin and tetracycline resistance gene cartridges. The vlpA-negative mutant has been assessed for virulence in the infant mouse cholera model.
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Towards Novel Methods of Mutagenesis for Histophilus somni
… system is needed. Mutagenesis of H. somni using allelic exchange is difficult due to its tight restriction modification system. Mutagenesis by natural transformation in Haemophilus influenzae is well established and may be enhanced by the presence of uptake signal sequences (USS) within the …
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Creating a Marked Mycobacterium avium subsp. paratuberculosis Vaccine Strain and Detecting Marker-Specific Immune Responses in Calves
… epitope-tagged immunogenic gene (PepA) via allelic exchange. Once gene replacement was confirmed, these markers were evaluated in a calf infection trial, where 17 Holstein-Friesian dairy calves were inoculated with either 109 CFUs of the marked strain (n = 6), 109 CFUs of a wild-type (WT) …
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DNA Repair of 8-oxo-7,8-Dihydroguanine Lesions in Porphyromonas gingivalis
… and used to create a uvrB-deficient mutant by allelic exchange. When plated on Brucella blood agar, the mutant strain, designated P. gingivalis FLL144, was similar in black-pigmentation and beta-hemolysis when compared to the parent strain. In addition, P. gingivalis FLL144 demonstrated no …
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Immunological and Molecular Analyses of the Borrelia burgdorferi OspF Protein Family F
… determine if inactivation of ospF genes through allelic exchange mutagenesis impacts on the pathogenicity of the Lyme disease spirochetes.
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Genetic Manipulation of Bacteroides for Transient Colonization of Germ-free Mice
… tracts. I designed deletion alleles that would exchange with the functional allele of target genes, and transferred the allelic exchange vector, pExchange, containing the deletion allele into B. thetaiotaomicron through conjugation. The double and triple gene deletion mutants failed to grow when …
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Characterisation and secretion mechanism of Bordetella pertussis autotransporter proteins
… mutants in two strains of B. pertussis. An allelic exchange procedure with the suicide vector pSS1129 carrying the bap-5 gene disrupted with a kanamycin-resistance cassette was used. PCR and Southern blotting confirmed the replacement of the wild-type bap-5 gene with the mutated version. …
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Site-Directed Mutagenesis in Francisella Tularensis by Allelic
… this study, a plasmid and method for generating allelic exchange mutants is reported, which should be useful for generating additional mutants of F. tularensis for use in clarifing the roles of specific genes. This vector is currently being used to make a nonencapsulated mutant of a virulent type …
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The capsular polysaccharide of Actinobacillus pleuropneumoniae serotype 5A: role in serum resistance and characterization of the genetic basis for expression
… partially or completely deleted was generated by allelic exchange. This mutant did not produce intracellular or extracellular CP, was serum-sensitive, and was attenuated in pigs. These studies demonstrated that CP contributed to the serum-resistance and virulence of A. pleuropneumoniae. This …
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The role of genomic islands in virulence of Pectobacterium carotovorum subspecies brasiliensis on potatoes
… ICMP19477, knockout mutants were constructed by allelic exchange mutagenesis. Mutations in the non-ribosomal peptide synthetase cluster of P. carotovorum subsp. brasiliensis (on PbN1_GI24) did not result in significant differences in virulence of the wild type and the mutant in pathogenicity …
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Cyclic-di-GMP Signaling in the Borrelia Spirochetes
… mammalian host. We next constructed two deletion/allelic exchange mutants – one with the targeted deletion of PlzA, and on ethat replaced PlzA with PlzB in a strain lacking the plzB gene. Our studies demonstrated that ΔplzA was deficient in motility and was also non-infectious in the mouse model …
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Exploring a Novel Anti-Gonococcal Compound for Combating Drug-Resistant <i>Neisseria gonorrhoeae</i>
The Gram-negative bacterium Neisseria gonorrhoeae, the causative agent of gonorrhea, is a significant public health concern due to its rapid development of resistance to antibiotics, leading to limited treatment options and potentially untreatable infections. This pathogen primarily infects the …