Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 175 for “"agrobacterium"”.
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Agrobacterium-mediated gene transfer into kiwifruit
… been developed to aid in the establishment of Agrobacterium-mediated transformation for new plant species. A series of binary vectors have been constructed that express a chimaeric β-D-glucuronidase (GUS) gene in plants cells but not in bacterial cells. This feature allows GUS activity from …
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Comparative Analysis of Flagellotropic Agrobacterium Phages
… and their bacterial hosts: Milano and host Agrobacterium tumefaciens str. C58, OLIVR4 and host Agrobacterium Biovar 1 str. ST15.13/040, and 7-7-1 and host Agrobacterium sp. H13-3. Chapter 1 is a literature review. Chapter 2 discusses the identification of the bacterial lipopolysaccharide as …
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Analysis of factors affecting the virulence of Agrobacterium tumefaciens
The Ti plasmid of Agrobacterium tumefaciens T37 was reconstructed in several different plasmids. The performance of the border repeats alone and in conjunction with overdrive and a left border inhibitory region was measured in the potato disc assay. These results were compared to T-strand …
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Agrobacterium-Mediated Transformation of Soybean: Test of Selectable Marker Genes
Soybean (Glycine max) is one of the most important crops around the world. Genetic modification of soybean offers an alternative way to augment the traditional soybean breeding approaches. Soybean transformation still remains a great challenge. To increase soybean transformation efficiency, three …
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Characterization of the VtlR regulons in Brucella abortus and Agrobacterium tumefaciens
Brucella abortus and Agrobacterium tumefaciens are pathogenic bacteria that infect animals and plants, respectively. These bacteria are genetically similar and are found within the same Class, Alphaproteobacteria, and Order, Rhizobiales, of the domain Eubacteria; however, they survive and replicate …
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Analyse von Protein-Protein Interaktionen im Typ IV Sekretionssystem von Agrobacterium tumefaciens
Agrobacterium tumefaciens ist ein Gram-negatives Bodenbakterium, das dikotyle Pflanzen infiziert. Mittels eines Typ IV Sekretionssystems werden dabei ein Teil der bakteriellen DNA (T-DNA), sowie die Virulenzproteine VirE2, VirE3, VirD2 und VirF in die pflanzliche Zelle transferiert. Das Typ IV …
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Improved regeneration and Agrobacterium-mediated transformation of wild strawberry (Fragaria vesca L.)
… work was to develop an efficient high-throughput Agrobacterium-mediated transformation protocol to generate an insertional mutant population to support the justification of F. vesca as a model organism for rosaceous crops. The transformation techniques described by Alsheikh et al. (2002) and …
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PopZ and FtsZ coordinate polar growth termination and cell division in Agrobacterium tumefaciens
… wall synthesis in the bacterial plant pathogen, Agrobacterium tumefaciens. Since cell wall (peptidoglycan) biosynthesis during elongation and cell division is vital for bacterial survival, we expected many key proteins involved in these processes to be essential for cell survival. Thus, we …
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Characterization of The Agrobacterium Tumefaciens Virb2 Pilin of The Virb/D4 Type Iv Secretion System
The <em>Agrobacterium</em> <em>tumefaciens</em> VirB/D4 type IV secretion system (T4SS) delivers oncogenic T-DNA and effector proteins to susceptible plant cells. This leads to the formation of tumors termed Crown Galls. The VirB/D4 T4SS is comprised of 12 subunits (VirB1 to VirB11 and VirD4), …
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Molecular, genetic, and biochemical studies of mannityl opine utilization by Agrobacterium tumefaciens: Its evolutionary implications
Agrobacterium strain NT1 harboring pSaB4, which contains the 14-kb BamHI fragment 4 from the octopine/mannityl opine-type Ti plasmid pTi15955, grows well with agropine (AGR). However, this strain grows slowly with mannopine (MOP) as sole carbon source, but gives rise to spontaneous mutants which …
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Physical, genetic, and biochemical analyses of mannityl opine catabolism genes from the Agrobacterium Ti plasmid pTi15955
We have isolated and characterized Tn3HoHo1- and Tn5-induced mutants of a cosmid clone, pYDH208, which encodes the mannopine cyclase-associated catabolism of mannopine (MOP) and agropine (AGR). Characterization of the transposon-induced lacZ fusion mutants by $\beta$-galactosidase activity and …
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The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation
The transfer of T-DNA from Agrobacterium tumefaciens to eukaryotic cells is on of the most extensively studied examples of interkingdom DNA transfer. This transfer system has revolutionized plant molecular biology to the extent that the introduction of foreign genes into plant genomes is now a …
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Effect of krilium on the respiratory activities of Rhizobium trifolii and Agrobacterium tumefaciens on various substrates
The respiratory rates of Rhizobium trifolii and Agrobacterium tumefaciens were measured by use of a Warburg Respirometer. A non-proliferating or "resting cell" technique was utilized in demonstrating the effect of Krilium on the oxygen consumption of the organisms when Krilium was aided to a given …
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Expression of Gus Gene in Tobacco (Nicotiana tabacum) and Corn (Zea mays) Using Dimboa-Resistant Agrobacterium Strains
<p>This thesis evaluates the ability of Agrobacterium tumefaciens strains resistant to the compound DIMBOA [2, 4-dihydroxy-7-methoxy-2h-l,4- benzoxazin-3(4H)-one] to infect corn. DIMBOA is a secondary plant metabolite and is believed to have antimicrobial properties and provides resistance to the …
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Characterization of Theacc (Agrocinopine Catabolism) Locus on the Nopaline-Type Ti Plasmid From Agrobacterium Tumefaciens Strain C58
… elements located in the promoter region of accR. Agrobacterium strains were attracted to several opines tested. The determinants for chemotaxis to these opines were localized to opine catabolic regions of the Ti plasmids. Analysis of insertion mutations and subclones of acc showed that accA, which …
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